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. 1982 Aug;151(2):534–541. doi: 10.1128/jb.151.2.534-541.1982

Wavelength dependence of energy transduction in Rhodopseudomonas sphaeroides: action spectrum of growth.

K J Hellingwerf, W de Vrij, W N Konings
PMCID: PMC220293  PMID: 6980219

Abstract

We determined the wavelength dependence of the specific growth rate of Rhodopseudomonas sphaeroides (the action spectrum of growth). A half-maximal (light-limited) growth rate was obtained when the culture vessel was illuminated with photon intensities between 0.8 x 10(14) and 3.5 x 10(14) photons cm-2 s-1 in the wavelength region between 400 and 950 nm. In the action spectrum, measured at 1.25 x 10(14) photons cm-2 s-1, distinct peaks could be observed at 480, 580, 800, and 870 nm, and minima could be found at 420, 540, 640 to 730, 830, and 940 nm. Both the pigment content and pigment composition of R. sphaeroides varied, depending on the wavelengths of the actinic light used for growth. This demonstrates that chromatic adaptation occurs in this bacterium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aagaard J., Sistrom W. R. Control of synthesis of reaction center bacteriochlorophyll in photosynthetic bacteria. Photochem Photobiol. 1972 Feb;15(2):209–225. doi: 10.1111/j.1751-1097.1972.tb06240.x. [DOI] [PubMed] [Google Scholar]
  2. Bennett A., Bogorad L. Complementary chromatic adaptation in a filamentous blue-green alga. J Cell Biol. 1973 Aug;58(2):419–435. doi: 10.1083/jcb.58.2.419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. COHEN-BAZIRE G., SISTROM W. R., STANIER R. Y. Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Physiol. 1957 Feb;49(1):25–68. doi: 10.1002/jcp.1030490104. [DOI] [PubMed] [Google Scholar]
  4. Gürgün V., Kirchner G., Pfennig N. Vergärung von Pyruvat durch sieben Arten phototropher Purpurbakterien. Z Allg Mikrobiol. 1976;16(8):573–586. doi: 10.1002/jobm.3630160802. [DOI] [PubMed] [Google Scholar]
  5. Hellingwerf K. J., Michels P. A., Dorpema J. W., Konings W. N. Transport of amino acids in membrane vesicles of Rhodopseudomonas spheroides energized by respiratory and cyclic electron flow. Eur J Biochem. 1975 Jul 1;55(2):397–406. doi: 10.1111/j.1432-1033.1975.tb02175.x. [DOI] [PubMed] [Google Scholar]
  6. Iwatsuki N., Joe C. O., Werbin H. Evidence that deoxyribonucleic acid photolyase from baker's yeast is a flavoprotein. Biochemistry. 1980 Mar 18;19(6):1172–1176. doi: 10.1021/bi00547a021. [DOI] [PubMed] [Google Scholar]
  7. LASCELLES J. The synthesis of porphyrins and bacteriochlorophyll by cell suspensions of Rhodopseudomonas spheroides. Biochem J. 1956 Jan;62(1):78–93. doi: 10.1042/bj0620078. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Morita S. Evidence for three photochemical systems in Chromatium D. Biochim Biophys Acta. 1968 Jan 15;153(1):241–247. doi: 10.1016/0005-2728(68)90166-7. [DOI] [PubMed] [Google Scholar]
  9. Pfennig N. Photosynthetic bacteria. Annu Rev Microbiol. 1967;21:285–324. doi: 10.1146/annurev.mi.21.100167.001441. [DOI] [PubMed] [Google Scholar]
  10. SAMEJIMA H., MYERS J. On the heterotrophic growth of Chlorella pyrenoidosa. J Gen Microbiol. 1958 Feb;18(1):107–117. doi: 10.1099/00221287-18-1-107. [DOI] [PubMed] [Google Scholar]
  11. SISTROM W. R. A requirement for sodium in the growth of Rhodopseudomonas spheroides. J Gen Microbiol. 1960 Jun;22:778–785. doi: 10.1099/00221287-22-3-778. [DOI] [PubMed] [Google Scholar]
  12. Sutherland B. M., Hausrath S. G. Multiple loci affecting photoreactivation in Escherichia coli. J Bacteriol. 1979 May;138(2):333–338. doi: 10.1128/jb.138.2.333-338.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ułaszewski S., Mamouneas T., Shen W. K., Rosenthal P. J., Woodward J. R., Cirillo V. P., Edmunds L. N., Jr Light effects in yeast: evidence for participation of cytochromes in photoinhibition of growth and transport in Saccharomyces cerevisiae cultured at low temperatures. J Bacteriol. 1979 May;138(2):523–529. doi: 10.1128/jb.138.2.523-529.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. White D., Shropshire W., Jr, Stephens K. Photocontrol of development by Stigmatella aurantiaca. J Bacteriol. 1980 Jun;142(3):1023–1024. doi: 10.1128/jb.142.3.1023-1024.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Zaugg W. S., Vernon L. P., Helmer G. Light-induced electron transfer reactions and adenosine triphosphate formation by Rhodospirillum rubrum chromatophores. Arch Biochem Biophys. 1967 Mar;119(1):560–571. doi: 10.1016/0003-9861(67)90491-2. [DOI] [PubMed] [Google Scholar]
  16. de Marsac N. T., Castets A. M., Cohen-Bazire G. Wavelength modulation of phycoerythrin synthesis in Synechocystis sp. 6701. J Bacteriol. 1980 Apr;142(1):310–314. doi: 10.1128/jb.142.1.310-314.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

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