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. 1971 Mar 1;57(3):297–302. doi: 10.1085/jgp.57.3.297

Do Unique Proteins Exist in Taste Buds?

Noriyuki Koyama 1, Kenzo Kurihara 1
PMCID: PMC2203100  PMID: 5544796

Abstract

Proteins in papillae on the bovine tongue were analyzed by semi-micro, polyacrylamide gel electrophoresis. All the proteins in the papillae with taste buds were observed to be common to proteins in the surrounding epithelium without taste buds. The protein band which was reported to form a weak complex with compounds called sweet by man was also found in all parts of the tongue epithelium. The receptor molecules for chemical stimuli may be distributed in all the cells of the tongue epithelium or the content of receptor molecules in taste bud papillae may be extremely low.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEIDLER L. M. A theory of taste stimulation. J Gen Physiol. 1954 Nov 20;38(2):133–139. doi: 10.1085/jgp.38.2.133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chrambach A., Reisfeld R. A., Wyckoff M., Zaccari J. A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis. Anal Biochem. 1967 Jul;20(1):150–154. doi: 10.1016/0003-2697(67)90272-2. [DOI] [PubMed] [Google Scholar]
  3. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  4. Dastoli F. R., Lopiekes D. V., Price S. A sweet-sensitive protein from bovine taste buds. Purification and partial characterization. Biochemistry. 1968 Mar;7(3):1160–1164. doi: 10.1021/bi00843a037. [DOI] [PubMed] [Google Scholar]
  5. Dastoli F. R., Price S. Sweet-sensitive protein from bovine taste buds: isolation and assay. Science. 1966 Nov 18;154(3751):905–907. doi: 10.1126/science.154.3751.905. [DOI] [PubMed] [Google Scholar]
  6. GORNALL A. G., BARDAWILL C. J., DAVID M. M. Determination of serum proteins by means of the biuret reaction. J Biol Chem. 1949 Feb;177(2):751–766. [PubMed] [Google Scholar]
  7. Hiji Y., Kobayashi N., Sato M. A "sweet-sensitive protein" from the tongue of the rat. Kumamoto Med J. 1968 Sep 30;21(3):137–139. [PubMed] [Google Scholar]
  8. Hiji Y., Kobayashi N., Sato M. Binding capacities of sugars with the "sweet-sensitive protein" from the rat tongue. Kumamoto Med J. 1969 Jun 30;22(2):104–107. [PubMed] [Google Scholar]
  9. LEBOY P. S., COX E. C., FLAKS J. G. THE CHROMOSOMAL SITE SPECIFYING A RIBOSOMAL PROTEIN IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1964 Dec;52:1367–1374. doi: 10.1073/pnas.52.6.1367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. REISFELD R. A., LEWIS U. J., WILLIAMS D. E. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels. Nature. 1962 Jul 21;195:281–283. doi: 10.1038/195281a0. [DOI] [PubMed] [Google Scholar]

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