Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1982 Aug;151(2):701–707. doi: 10.1128/jb.151.2.701-707.1982

Plasmid loss and changes within the chromosomal DNA of Streptomyces reticuli.

H Schrempf
PMCID: PMC220311  PMID: 7096267

Abstract

The sporulating wild-type strain of Streptomyces reticuli, which produces a melanin pigment and the macrolide leucomycin, contains plasmid DNA of 48 to 49 megadaltons. Plasmidless variants had an altered secondary metabolism and a changed antibiotic resistance pattern. By using a new colony hybridization technique developed for streptomycetes, it could be shown that plasmidless variants could be transformed with the wild-type plasmid DNA, which, however, is quickly lost from regenerated mycelium. In contrast to the wild-type strain, the plasmidless variants contain amplified nucleotide sequences within the chromosomal DNA. The number and size of these sequences vary with the strain tested. Hybridization studies revealed that the reiterated sequences are neither amplified ribosomal nor plasmid genes, but are present in small concentrations within the wild-type chromosome. Some of them share extensive homologies with each other and are located at different positions within the chromosome. It is assumed that alterations in secondary metabolism are due to changes within both the chromosomal and the extrachromosomal DNAs of S. reticuli.

Full text

PDF
701

Images in this article

704Image
on p.704
705Image
on p.705
706Image
on p.706
707Image
on p.707

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akagawa H., Okanishi M., Umezawa H. A plasmid involved in chloramphenicol production in Streptomyces venezuelae: evidence from genetic mapping. J Gen Microbiol. 1975 Oct;90(2):336–346. doi: 10.1099/00221287-90-2-336. [DOI] [PubMed] [Google Scholar]
  2. Arrand J. R., Myers P. A., Roberts R. J. A new restriction endonuclease from Streptomyces albus G. J Mol Biol. 1978 Jan 5;118(1):127–135. doi: 10.1016/0022-2836(78)90249-8. [DOI] [PubMed] [Google Scholar]
  3. Bibb M. J., Ward J. M., Hopwood D. A. Transformation of plasmid DNA into Streptomyces at high frequency. Nature. 1978 Jul 27;274(5669):398–400. doi: 10.1038/274398a0. [DOI] [PubMed] [Google Scholar]
  4. Helling R. B., Goodman H. M., Boyer H. W. Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis. J Virol. 1974 Nov;14(5):1235–1244. doi: 10.1128/jvi.14.5.1235-1244.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hopwood D. A., Merrick M. J. Genetics of antibiotic production. Bacteriol Rev. 1977 Sep;41(3):595–635. doi: 10.1128/br.41.3.595-635.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lerch K., Ettinger L. Purification and characterization of a tyrosinase from Streptomyces glaucescens. Eur J Biochem. 1972 Dec 18;31(3):427–437. doi: 10.1111/j.1432-1033.1972.tb02549.x. [DOI] [PubMed] [Google Scholar]
  7. O'Callaghan C. H., Morris A., Kirby S. M., Shingler A. H. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother. 1972 Apr;1(4):283–288. doi: 10.1128/aac.1.4.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Okanishi M., Suzuki K., Umezawa H. Formation and reversion of Streptomycete protoplasts: cultural condition and morphological study. J Gen Microbiol. 1974 Feb;80(2):389–400. doi: 10.1099/00221287-80-2-389. [DOI] [PubMed] [Google Scholar]
  9. Omura S., Nakagawa A. Chemical and biological studies on 16-membered macrolide antibiotics. J Antibiot (Tokyo) 1975 Jun;28(6):401–433. doi: 10.7164/antibiotics.28.401. [DOI] [PubMed] [Google Scholar]
  10. Ratcliff S. W., Luh J., Ganesan A. T., Behrens B., Thompson R., Montenegro M. A., Morelli G., Trautner T. A. The genome of Bacillus subtilis phage SPP1: the arrangement of restriction endonuclease generated fragments. Mol Gen Genet. 1979 Jan 10;168(2):165–172. doi: 10.1007/BF00431442. [DOI] [PubMed] [Google Scholar]
  11. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  12. Robinson M., Lewis E., Napier E. Occurrence of reiterated DNA sequences in strains of Streptomyces produced by an interspecific protoplast fusion. Mol Gen Genet. 1981;182(2):336–340. doi: 10.1007/BF00269680. [DOI] [PubMed] [Google Scholar]
  13. Schrempf H., Bujard H., Hopwood D. A., Goebel W. Isolation of covalently closed circular deoxyribonucleic acid from Streptomyces coelicolor A3(2). J Bacteriol. 1975 Feb;121(2):416–421. doi: 10.1128/jb.121.2.416-421.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schrempf H., Goebel W. Characterization of a plasmid from Streptomyces coelicolor A3(2). J Bacteriol. 1977 Jul;131(1):251–258. doi: 10.1128/jb.131.1.251-258.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES