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. 1982 Aug;151(2):718–722. doi: 10.1128/jb.151.2.718-722.1982

Roles of lipopolysaccharide and outer membrane protein OmpC of Escherichia coli K-12 in the receptor function for bacteriophage T4.

F Yu, S Mizushima
PMCID: PMC220313  PMID: 7047495

Abstract

The roles of lipopolysaccharide and OmpC, a major outer membrane protein, in the receptor function for bacteriophage T4 were studied by using Escherichia coli K-12 strains having mutations in the ompC gene or in genes controlling different stages of lipopolysaccharide synthesis. The receptor activity for T4 was monitored by (i) T4 sensitivity of intact cells, (ii) phage inactivation activity of cell envelopes, and (iii) phage inactivation activity of specimens reconstituted from purified OmpC and lipopolysaccharide. It was found that (i) in the presence of the OmpC protein, the essential region of the lipopolysaccharide for the receptor activity was the core-lipid A region that includes the heptose region, whereas the glucose region was not necessarily required for the receptor function; (ii) the OmpC protein was not required at all when the distal end of the lipopolysaccharide was removed to expose a glucose residue at the distal end; and (iii) when cells lacked both the OmpC protein and the glucose region, they became extremely resistant to T4. Based on these findings, the roles of the OmpC protein and lipopolysaccharide in T4 infection are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boman H. G., Monner D. A. Characterization of lipopolysaccharides from Escherichia coli K-12 mutants. J Bacteriol. 1975 Feb;121(2):455–464. doi: 10.1128/jb.121.2.455-464.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chai T. J., Foulds J. Escherichia coli K-12 tolF mutants: alterations in protein composition of the outer membrane. J Bacteriol. 1977 May;130(2):781–786. doi: 10.1128/jb.130.2.781-786.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dawes J. Characterisation of the bacteriophage T4 receptor site. Nature. 1975 Jul 10;256(5513):127–128. doi: 10.1038/256127a0. [DOI] [PubMed] [Google Scholar]
  4. Furukawa H., Mizushima S. Roles of cell surface components of Escherichia coli K-12 in bacteriophage T4 infection: interaction of tail core with phospholipids. J Bacteriol. 1982 May;150(2):916–924. doi: 10.1128/jb.150.2.916-924.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Furukawa H., Yamada H., Mizushima S. Interaction of bacteriophage T4 with reconstituted cell envelopes of Escherichia coli K-12. J Bacteriol. 1979 Dec;140(3):1071–1080. doi: 10.1128/jb.140.3.1071-1080.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Galanos C., Lüderitz O., Westphal O. A new method for the extraction of R lipopolysaccharides. Eur J Biochem. 1969 Jun;9(2):245–249. doi: 10.1111/j.1432-1033.1969.tb00601.x. [DOI] [PubMed] [Google Scholar]
  7. Henning U., Jann K. Two-component nature of bacteriophage T4 receptor activity in Escherichia coli K-12. J Bacteriol. 1979 Jan;137(1):664–666. doi: 10.1128/jb.137.1.664-666.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Miyoshi Y., Yamagata H. Sucrose-dependent spectinomycin-resistant mutants of Escherichia coli. J Bacteriol. 1976 Jan;125(1):142–148. doi: 10.1128/jb.125.1.142-148.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mutoh N., Furukawa H., Mizushima S. Role of lipopolysaccharide and outer membrane protein of Escherichia coli K-12 in the receptor activity for bacteriophage T4. J Bacteriol. 1978 Nov;136(2):693–699. doi: 10.1128/jb.136.2.693-699.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mutoh N., Nagasawa T., Mizushima S. Specialized transducing bacteriophage lambda carrying the structural gene for a major outer membrane matrix protein of Escherichia coli K-12. J Bacteriol. 1981 Feb;145(2):1085–1090. doi: 10.1128/jb.145.2.1085-1090.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nakamura K., Mizushima S. Effects of heating in dodecyl sulfate solution on the conformation and electrophoretic mobility of isolated major outer membrane proteins from Escherichia coli K-12. J Biochem. 1976 Dec;80(6):1411–1422. doi: 10.1093/oxfordjournals.jbchem.a131414. [DOI] [PubMed] [Google Scholar]
  13. Osborn M. J., Gander J. E., Parisi E., Carson J. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane. J Biol Chem. 1972 Jun 25;247(12):3962–3972. [PubMed] [Google Scholar]
  14. Prehm P., Jann B., Jann K., Schmidt G., Stirm S. On a bacteriophage T3 and T4 receptor region within the cell wall lipopolysaccharide of Escherichia coli B. J Mol Biol. 1976 Feb 25;101(2):277–281. doi: 10.1016/0022-2836(76)90377-6. [DOI] [PubMed] [Google Scholar]
  15. Prehm P., Stirm S., Jann B., Jann K., Boman H. G. Cell-wall lipopolysaccharides of ampicillin-resistant mutants of Escherichia coli K-12. Eur J Biochem. 1976 Jul 1;66(2):369–377. doi: 10.1111/j.1432-1033.1976.tb10526.x. [DOI] [PubMed] [Google Scholar]
  16. Prehm P., Stirm S., Jann B., Jann K. Cell-wall lipopolysaccharide from Escherichia coli B. Eur J Biochem. 1975 Aug 1;56(1):41–55. doi: 10.1111/j.1432-1033.1975.tb02205.x. [DOI] [PubMed] [Google Scholar]
  17. Wilson J. H., Luftig R. B., Wood W. B. Interaction of bacteriophage T4 tail fiber components with a lipopolysaccharide fraction from Escherichia coli. J Mol Biol. 1970 Jul 28;51(2):423–434. doi: 10.1016/0022-2836(70)90152-x. [DOI] [PubMed] [Google Scholar]
  18. Wright B. G., Rebers P. A. Procedure for determining heptose and hexose in lipopolysaccharides. Modification of the cysteine-sulfuric acid method. Anal Biochem. 1972 Oct;49(2):307–319. doi: 10.1016/0003-2697(72)90433-2. [DOI] [PubMed] [Google Scholar]
  19. Yamada H., Mizushima S. Reconstitution of an ordered structure from major outer membrane constituents and the lipoprotein-bearing peptidoglycan sacculus of Escherichia coli. J Bacteriol. 1978 Sep;135(3):1024–1031. doi: 10.1128/jb.135.3.1024-1031.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Yu F., Ichihara S., Mizushima S. A major outer membrane protein (O-8) of Escherichia coli K-12 exists as a trimer in sodium dodecyl sulfate solution. FEBS Lett. 1979 Apr 1;100(1):71–74. doi: 10.1016/0014-5793(79)81133-3. [DOI] [PubMed] [Google Scholar]
  21. Yu F., Yamada H., Mizushima S. Role of lipopolysaccharide in the receptor function for bacteriophage TuIb in Escherichia coli. J Bacteriol. 1981 Nov;148(2):712–715. doi: 10.1128/jb.148.2.712-715.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

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