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. 1972 Apr 1;59(4):401–414. doi: 10.1085/jgp.59.4.401

Rhodopsin of the Larval Mosquito

Paul K Brown 1, Richard H White 1
PMCID: PMC2203182  PMID: 5029551

Abstract

Larvae of the mosquito Aedes aegypti have a cluster of four ocelli on each side of the head. The visual pigment of each ocellus of mosquitoes reared in darkness was characterized by microspectrophotometry, and found to be the same. Larval mosquito rhodopsin (λmax = 515 nm) upon short irradiation bleaches to a stable photoequilibrium with metarhodopsin (λmax = 480 nm). On long irradiation of glutaraldehyde-fixed tissues or in the presence of potassium borohydride, bleaching goes further, and potassium borohydride reduces the product, retinal, to retinol (vitamin A1). In the presence of hydroxylamine, the rhodopsin bleaches rapidly, with conversion of the chromophore to retinaldehyde oxime (λmax about 365 nm).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOWNESS J. M., WOLKEN J. J. A light-sensitive yellow pigment from the housefly. J Gen Physiol. 1959 Mar 20;42(4):779–792. doi: 10.1085/jgp.42.4.779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BROWN P. K., BROWN P. S. Visual pigments of the octopus and cuttlefish. Nature. 1958 Nov 8;182(4645):1288–1290. doi: 10.1038/1821288a0. [DOI] [PubMed] [Google Scholar]
  3. DARTNALL H. J. A. The interpretation of spectral sensitivity curves. Br Med Bull. 1953;9(1):24–30. doi: 10.1093/oxfordjournals.bmb.a074302. [DOI] [PubMed] [Google Scholar]
  4. GOLDSMITH T. H. On the visual system of the bee (Apis mellifera). Ann N Y Acad Sci. 1959 Nov 12;74(2):223–229. doi: 10.1111/j.1749-6632.1958.tb39547.x. [DOI] [PubMed] [Google Scholar]
  5. Goldsmith T. H. THE VISUAL SYSTEM OF THE HONEYBEE. Proc Natl Acad Sci U S A. 1958 Feb;44(2):123–126. doi: 10.1073/pnas.44.2.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HUBBARD R., ST GEORGE R. C. The rhodopsin system of the squid. J Gen Physiol. 1958 Jan 20;41(3):501–528. doi: 10.1085/jgp.41.3.501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Langer H., Thorell B. Microspectrophotometry of single rhabdomeres in the insect eye. Exp Cell Res. 1966 Mar;41(3):673–677. doi: 10.1016/s0014-4827(66)80119-2. [DOI] [PubMed] [Google Scholar]
  8. MATTHEWS R. G., HUBBARD R., BROWN P. K., WALD G. TAUTOMERIC FORMS OF METARHODOPSIN. J Gen Physiol. 1963 Nov;47:215–240. doi: 10.1085/jgp.47.2.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Seldin E. B., White R. H., Brown P. K. Spectral sensitivity of larval mosquito ocelli. J Gen Physiol. 1972 Apr;59(4):415–420. doi: 10.1085/jgp.59.4.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. WALD G., BROWN P. K., GIBBONS I. R. The problem of visual excitation. J Opt Soc Am. 1963 Jan;53:20–35. doi: 10.1364/josa.53.000020. [DOI] [PubMed] [Google Scholar]
  11. WALD G., BROWN P. K. Synthesis and bleaching of rhodopsin. Nature. 1956 Jan 28;177(4500):174–176. doi: 10.1038/177174a0. [DOI] [PubMed] [Google Scholar]
  12. WALD G., BROWN P. K. The molar extinction of rhodopsin. J Gen Physiol. 1953 Nov 20;37(2):189–200. doi: 10.1085/jgp.37.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. WALD G., HUBBARD R. Visual pigment of a decapod crustacean: the lobster. Nature. 1957 Aug 10;180(4580):278–280. doi: 10.1038/180278a0. [DOI] [PubMed] [Google Scholar]
  14. WOLKEN J. J., SCHEER I. J. An eye pigment of the cockroach. Exp Eye Res. 1963 Apr;2:182–188. doi: 10.1016/s0014-4835(63)80011-1. [DOI] [PubMed] [Google Scholar]
  15. Wald G., Brown P. K. The Synthesis of Rhodopsin from Retinene(1). Proc Natl Acad Sci U S A. 1950 Feb;36(2):84–92. doi: 10.1073/pnas.36.2.84. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Waterman T. H., Fernández H. R., Goldsmith T. H. Dichroism of photosensitive pigment in rhabdoms of the crayfish Orconectes. J Gen Physiol. 1969 Sep;54(3):415–432. doi: 10.1085/jgp.54.3.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. White R. H., Sundeen C. D. The effect of light and light deprivation upon the ultrastructure of the larval mosquito eye. I. Polyribosomes and endoplasmic reticulum. J Exp Zool. 1967 Apr;164(3):461–477. doi: 10.1002/jez.1401640312. [DOI] [PubMed] [Google Scholar]
  18. White R. H. The effect of light and light deprivation upon the ultrastructure of the larval mosquito eye. 3. Multivesicular bodies and protein uptake. J Exp Zool. 1968 Nov;169(3):261–277. doi: 10.1002/jez.1401690302. [DOI] [PubMed] [Google Scholar]
  19. White R. H. The effect of light and light deprivation upon the ultrastructure of the larval mosquito eye. II. The rhabdom. J Exp Zool. 1967 Dec;166(3):405–425. doi: 10.1002/jez.1401660313. [DOI] [PubMed] [Google Scholar]

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