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. 1973 Jan 1;61(1):110–124. doi: 10.1085/jgp.61.1.110

The Effect of Hypertonic Media on Water Permeability of Frog Urinary Bladder

Inhibition by catecholamines and prostaglandin E1

P Ripoche 1, J Bourguet 1, M Parisi 1
PMCID: PMC2203462  PMID: 4345637

Abstract

The frog urinary bladder undergoes, in some conditions, a marked increase of its water permeability when incubated in hypertonic media. This increase was observed with various nonpermeant solutes. It seems to result from the shrinkage of an osmo-sensitive compartment of the tissue, probably the epithelial cells. Many similarities were found between this effect and the physiological increase in water permeability (hydrosmotic response) elicited by antidiuretic hormone (ADH): both were dependent on the physiological state of the animals, and although the response was slower after hyperosmolar than after hormonal challenge, the patterns of response were similar, and in both cases markedly dependent on bathing solution temperature. Norepinephrine and prostaglandin E1, which in this tissue reduce the hydrosmotic action of ADH, presumably by inhibiting the adenyl cylase also reduced the effect of hyperosmolarity. Conversely this effect was potentiated by incubation in the presence of oxytocin, exogenous cyclic AMP, and theophylline, conditions in which the intracellular concentration of cyclic AMP is increased. These data demonstrate that the response to hyperosmolarity is elicited, at least partly, by mechanisms also involved in the physiological hydrosmotic response to ADH.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENTLEY P. J. PHYSIOLOGICAL PROPERTIES OF THE ISOLATED FROG BLADDER IN HYPEROSMOTIC SOLUTIONS. Comp Biochem Physiol. 1964 Jun;12:233–239. doi: 10.1016/0010-406x(64)90177-x. [DOI] [PubMed] [Google Scholar]
  2. BOURGUET J., JARD S. UN DISPOSITIF AUTOMATIQUE DE MESURE ET D'ENREGISTREMENT DU FLUX NET D'EAU 'A TRAVERS LA PEAU ET LA VESSIE DES AMPHIBIENS. Biochim Biophys Acta. 1964 Sep 25;88:442–444. [PubMed] [Google Scholar]
  3. BOURGUET J., MAETZ J. [Arguments in favor of the independence of the mechanisms of action of various neurohypophyseal hormones on the osmotic flux of water and on the active transport of sodium in the same receptor; studies on the bladder and the skin of Rana esculenta L]. Biochim Biophys Acta. 1961 Sep 30;52:552–565. doi: 10.1016/0006-3002(61)90414-0. [DOI] [PubMed] [Google Scholar]
  4. Handler J. S., Bensinger R., Orloff J. Effect of adrenergic agents on toad bladder response to ADH, 3',5'-AMP, and theophylline. Am J Physiol. 1968 Nov;215(5):1024–1031. doi: 10.1152/ajplegacy.1968.215.5.1024. [DOI] [PubMed] [Google Scholar]
  5. MAETZ J., MOREL F., RACE B. [Demonstration in the neurohypophysis of Rana esculenta L. of a new hormonal factor stimulating the active transport of sodium through the skin]. Biochim Biophys Acta. 1959 Dec;36:317–326. doi: 10.1016/0006-3002(59)90173-8. [DOI] [PubMed] [Google Scholar]
  6. ORLOFF J., HANDLER J. S., BERGSTROM S. EFFECT OF PROSTAGLANDIN (PGE-1) ON THE PERMEABILITY RESPONSE OF TOAD BLADDER TO VASOPRESSIN, THEOPHYLLINE AND ADENOSINE 3',5'-MONOPHOSPHATE. Nature. 1965 Jan 23;205:397–398. doi: 10.1038/205397a0. [DOI] [PubMed] [Google Scholar]
  7. PETERSEN M. J., EDELMAN I. S. CALCIUM INHIBITION OF THE ACTION OF VASOPRESSIN ON THE URINARY BLADDER OF THE TOAD. J Clin Invest. 1964 Apr;43:583–594. doi: 10.1172/JCI104943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Rasmussen H., Schwartz I. L., Schoessler M. A., Hochster G. STUDIES ON THE MECHANISM OF ACTION OF VASOPRESSIN. Proc Natl Acad Sci U S A. 1960 Oct;46(10):1278–1287. doi: 10.1073/pnas.46.10.1278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ripoche P., Parisi M., Bourguet J. Effect of hypertonic media on the reversal of the hydrosmotic action of antidiuretic hormone in frog urinary bladder. Biochim Biophys Acta. 1971 Aug 13;241(2):716–718. doi: 10.1016/0005-2736(71)90075-7. [DOI] [PubMed] [Google Scholar]
  10. Ripoche P., Parisi M., Bourguet J. Mechanism of the "antidiuretic hormone-like" action of hypertonic media on the frog urinary bladder. Biochim Biophys Acta. 1969 Oct 14;193(1):231–234. doi: 10.1016/0005-2736(69)90080-7. [DOI] [PubMed] [Google Scholar]
  11. USSING H. H. RELATIONSHIP BETWEEN OSMOTIC REACTIONS AND ACTIVE SODIUM TRANSPORT IN THE FROG SKIN EPITHELIUM. Acta Physiol Scand. 1965 Jan-Feb;63:141–155. doi: 10.1111/j.1748-1716.1965.tb04052.x. [DOI] [PubMed] [Google Scholar]
  12. Urakabe S., Handler J. S., Orloff J. Effect of hypertonicity on permeability properties of the toad bladder. Am J Physiol. 1970 Apr;218(4):1179–1187. doi: 10.1152/ajplegacy.1970.218.4.1179. [DOI] [PubMed] [Google Scholar]
  13. Ussing H. H. Anomalous transport of electrolytes and sucrose through the isolated frog skin induced by hypertonicity of the outside bathing solution. Ann N Y Acad Sci. 1966 Jul 14;137(2):543–555. doi: 10.1111/j.1749-6632.1966.tb50180.x. [DOI] [PubMed] [Google Scholar]

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