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. 1973 Feb 1;61(2):158–175. doi: 10.1085/jgp.61.2.158

Tension in Isolated Frog Muscle Fibers Induced by Hypertonic Solutions

J Lännergren 1, J Noth 1
PMCID: PMC2203468  PMID: 4540058

Abstract

The effect of hypertonic solutions on the tension of isolated twitch muscle fibers of the frog has been investigated. Increased tonicity up to about 1.7 times normal (1.7 T) caused a very small, graded, maintained tension increase. Above about 1.7 T a large, transient contracture response was superimposed on the small tension. The contracture response was graded with tonicity and reached a maximum at 2.5 T of 108 ± 25 mN·mm2 a third of the maximum tetanic tension in isotonic solution. Contracture tension developed with a delay which decreased with increased tonicity. The contracture threshold was lower and the delay shorter in small fibers than in large. Contractures were obtained equally well in depolarized as in polarized fibers. They were completely suppressed by 0.1–0.5 mM tetracaine. The possible mechanism responsible for the tension-inducing effect of hypertonic solutions is discussed in terms of the close similarity between the properties of these contractures and those caused by caffeine, and it is suggested that the effect is due to a release of calcium from internal stores.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AXELSSON J., THESLEFF S. Activation of the contractile mechanism in striated muscle. Acta Physiol Scand. 1958 Oct 28;44(1):55–66. doi: 10.1111/j.1748-1716.1958.tb01608.x. [DOI] [PubMed] [Google Scholar]
  2. April E., Brandt P. W., Reuben J. P., Grundfest H. Muscle contraction: the effect of ionic strength. Nature. 1968 Oct 12;220(5163):182–184. doi: 10.1038/220182a0. [DOI] [PubMed] [Google Scholar]
  3. Birks R. I., Davey D. F. Osmotic responses demonstrating the extracellular character of the sarcoplasmic reticulum. J Physiol. 1969 May;202(1):171–188. doi: 10.1113/jphysiol.1969.sp008802. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Caputo C. Caffeine- and potassium-induced contractures of frog striated muscle fibers in hypertonic solutions. J Gen Physiol. 1966 Sep;50(1):129–139. doi: 10.1085/jgp.50.1.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ebashi S., Endo M. Calcium ion and muscle contraction. Prog Biophys Mol Biol. 1968;18:123–183. doi: 10.1016/0079-6107(68)90023-0. [DOI] [PubMed] [Google Scholar]
  6. FEINSTEIN M. B. INHIBITION OF CAFFEINE RIGOR AND RADIOCALCIUM MOVEMENTS BY LOCAL ANESTHETICS IN FROG SARTORIUS MUSCLE. J Gen Physiol. 1963 Sep;47:151–172. doi: 10.1085/jgp.47.1.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FRANK G. B. Effects of changes in extracellular calcium concentration on the potassium-induced contracture of frog's skeletal muscle. J Physiol. 1960 Jun;151:518–538. doi: 10.1113/jphysiol.1960.sp006457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FRANK G. B. Utilization of bound calcium in the action of caffeine and certain multivalent cations on skeletal muscle. J Physiol. 1962 Sep;163:254–268. doi: 10.1113/jphysiol.1962.sp006972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Feinstein M. B., Paimre M. Pharmacological action of local anesthetics on excitation-contraction coupling in striated and smooth muscle. Fed Proc. 1969 Sep-Oct;28(5):1643–1648. [PubMed] [Google Scholar]
  10. Frankenhaeuser B., Lännergren J. The effect of calcium on the mechanical response of single twitch muscle fibres of Xenopus laevis. Acta Physiol Scand. 1967 Mar;69(3):242–254. doi: 10.1111/j.1748-1716.1967.tb03518.x. [DOI] [PubMed] [Google Scholar]
  11. Gordon A. M., Godt R. E. Some effects of hypertonic solutions on contraction and excitation-contraction coupling in frog skeletal muscles. J Gen Physiol. 1970 Feb;55(2):254–275. doi: 10.1085/jgp.55.2.254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gruener R. Caffeine contractures in sarcolemma-free muscle fibres. J Physiol. 1967 Jul;191(2):106P–108P. [PubMed] [Google Scholar]
  13. HODGKIN A. L., HOROWICZ P. Potassium contractures in single muscle fibres. J Physiol. 1960 Sep;153:386–403. doi: 10.1113/jphysiol.1960.sp006541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. HODGKIN A. L., HOROWICZ P. The influence of potassium and chloride ions on the membrane potential of single muscle fibres. J Physiol. 1959 Oct;148:127–160. doi: 10.1113/jphysiol.1959.sp006278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. HOWARTH J. V. The behaviour of frog muscle in hypertonic solutions. J Physiol. 1958 Nov 10;144(1):167–175. doi: 10.1113/jphysiol.1958.sp006093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hill D. K. Tension due to interaction between the sliding filaments in resting striated muscle. The effect of stimulation. J Physiol. 1968 Dec;199(3):637–684. doi: 10.1113/jphysiol.1968.sp008672. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Isaacson A., Sandow A. Quinine and caffeine effects on 45Ca movements in frog sartorius muscle. J Gen Physiol. 1967 Sep;50(8):2109–2128. doi: 10.1085/jgp.50.8.2109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lännergren J. The effect of low-level activation on the mechanical properties of isolated frog muscle fibers. J Gen Physiol. 1971 Aug;58(2):145–162. doi: 10.1085/jgp.58.2.145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sorenson A. L. Water permeability of isolated muscle fibers of a marine crab. J Gen Physiol. 1971 Sep;58(3):287–303. doi: 10.1085/jgp.58.3.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. VARGA-MANYI P., TIGYI J. Separation of muscle excitation from contraction. Acta Physiol Acad Sci Hung. 1962;22:287–291. [PubMed] [Google Scholar]
  21. Weber A., Herz R. The relationship between caffeine contracture of intact muscle and the effect of caffeine on reticulum. J Gen Physiol. 1968 Nov;52(5):750–759. doi: 10.1085/jgp.52.5.750. [DOI] [PMC free article] [PubMed] [Google Scholar]

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