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. 1974 Apr 1;63(4):492–508. doi: 10.1085/jgp.63.4.492

Effect of Peptide PV on the Ionic Permeability of Lipid Bilayer Membranes

H P Ting-Beall 1, M T Tosteson 1, B F Gisin 1, D C Tosteson 1
PMCID: PMC2203559  PMID: 4820091

Abstract

This paper reports the effects of peptide PV (primary structure: cyclo-(D-val-L-pro-L-val-D-pro)δ) on the electrical properties of sheep red cell lipid bilayers. The membrane conductance (Gm) induced by PV in either Na+ or K+ medium is proportional to the concentration of PV in the aqueous phase. The PV concentration required to produce a comparable increase in Gm in K+ medium is about 104 times greater than for its analogue, valinomycin (val). Although the selectivity sequence for PV and val is similar, K+ ≳ Rb+ > Cs+ > NH4 + > TI+ > Na+ > Li+; the ratio of GGm in K+ to that in Na+ is about 10 for PV compared to > 103 for val. When equal concentrations of PV are added to both sides of a bilayer, the membrane current approaches a maximum value independent of voltage when the membrane potential exceeds 100 mV. When PV is added to only one side of a bilayer separating identical salt solutions of either Na+ or K+ salts, rectification occurs such that the positive current flows more easily away rather than toward the side containing the carrier. Under these conditions, a large, stable, zero-current potential (VVm) is also observed, with the side containing PV being negative. The magnitude of this VVm is about 90 mV and relatively independent of PV concentration when the latter is larger than 2 Times; 10–5 M. From a model which assumes that Vm equals the equilibrium potential for the PV-cation complexes (MS +) and that the reaction between PV and cations is at equilibrium on the two membrane surfaces, we compute the permeability of the membrane to free PV to be about 10–5 cm s–1, which is about 10–7 times the permeability of similar membranes to free val. This interpretation is supported by the fact that the observed values of Vm are in agreement with the calculated equilibrium potential for MS+ over a wide range of ratios of concentrations of total PV in the two bathing solutions, if the unstirred layers are taken into account in computing the MS+ concentrations at the membrane surfaces.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andreoli T. E., Bangham J. A., Tosteson D. C. The formation and properties of thin lipid membranes from HK and LK sheep red cell lipids. J Gen Physiol. 1967 Jul;50(6):1729–1749. doi: 10.1085/jgp.50.6.1729. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Andreoli T. E., Tieffenberg M., Tosteson D. C. The effect of valinomycin on the ionic permeability of thin lipid membranes. J Gen Physiol. 1967 Dec;50(11):2527–2545. doi: 10.1085/jgp.50.11.2527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gisin B. F., Merrifield R. B. Synthesis of a hydrophobic potassium binding peptide. J Am Chem Soc. 1972 Aug 23;94(17):6165–6170. doi: 10.1021/ja00772a039. [DOI] [PubMed] [Google Scholar]
  4. Gisin B. F., Merrifield R. B., Tosteson D. C. Solid-phase synthesis of the cyclododecadepsipeptide Valinomycin. J Am Chem Soc. 1969 May 7;91(10):2691–2695. doi: 10.1021/ja01038a047. [DOI] [PubMed] [Google Scholar]
  5. HUANG C., WHEELDON L., THOMPSON T. E. THE PROPERTIES OF LIPID BILAYER MEMBRANES SEPARATING TWO AQUEOUS PHASES: FORMATION OF A MEMBRANE OF SIMPLE COMPOSITION. J Mol Biol. 1964 Jan;8:148–160. doi: 10.1016/s0022-2836(64)80155-8. [DOI] [PubMed] [Google Scholar]
  6. Haydon D. A., Hladky S. B. Ion transport across thin lipid membranes: a critical discussion of mechanisms in selected systems. Q Rev Biophys. 1972 May;5(2):187–282. doi: 10.1017/s0033583500000883. [DOI] [PubMed] [Google Scholar]
  7. Hladky S. B. The effect of stirring on the flux of carriers into black lipid membranes. Biochim Biophys Acta. 1973 May 11;307(2):261–269. doi: 10.1016/0005-2736(73)90093-x. [DOI] [PubMed] [Google Scholar]
  8. Lieb W. R., Stein W. D. The influence of unstirred layers on the kinetics of carrier-mediated transport. J Theor Biol. 1972 Sep;36(3):641–645. doi: 10.1016/0022-5193(72)90016-1. [DOI] [PubMed] [Google Scholar]
  9. Mueller P., Rudin D. O. Development of K+-Na+ discrimination in experimental bimolecular lipid membranes by macrocyclic antibiotics. Biochem Biophys Res Commun. 1967 Feb 21;26(4):398–404. doi: 10.1016/0006-291x(67)90559-1. [DOI] [PubMed] [Google Scholar]
  10. Pressman B. C., Harris E. J., Jagger W. S., Johnson J. H. Antibiotic-mediated transport of alkali ions across lipid barriers. Proc Natl Acad Sci U S A. 1967 Nov;58(5):1949–1956. doi: 10.1073/pnas.58.5.1949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. VAN DEN BERG H. J. A NEW TECHNIQUE FOR OBTAINING THIN LIPID FILMS SEPARATING TWO AQUEOUS MEDIA. J Mol Biol. 1965 May;12:290–291. doi: 10.1016/s0022-2836(65)80302-3. [DOI] [PubMed] [Google Scholar]
  12. Winne D. Unstirred layer, source of biased Michaelis constant in membrane transport. Biochim Biophys Acta. 1973 Feb 27;298(1):27–31. doi: 10.1016/0005-2736(73)90005-9. [DOI] [PubMed] [Google Scholar]

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