Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1982 Sep;151(3):1204–1209. doi: 10.1128/jb.151.3.1204-1209.1982

Exonuclease activity from Pseudomonas aeruginosa which is missing in phenotypically restrictionless mutants.

A A Potter, J S Loutit
PMCID: PMC220397  PMID: 6286593

Abstract

A phenotypically restrictionless strain of Pseudomonas aeruginosa was found to lack a deoxyribonuclease specific for linear duplex DNA. The purified enzyme had an optimum pH of 8.5, required MgCl2 (10 mM) for maximum activity, and did not require ATP. Neither the degradation of heat-denatured DNA nor the degradation of bacteriophage F116 DNA was detected. The genome of bacteriophage F116 was shown to possess single-stranded terminal regions, which account for the resistance to degradation and for the ability of the phage to transfect restriction-proficient strains.

Full text

PDF
1204

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Clark A. J. Progress toward a metabolic interpretation of genetic recombination of Escherichia coli and bacteriophage lambda. Genetics. 1974 Sep;78(1):259–271. doi: 10.1093/genetics/78.1.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cosloy S. D., Oishi M. The nature of the transformation process in Escherichia coli K12. Mol Gen Genet. 1973 Jul 31;124(1):1–10. doi: 10.1007/BF00267159. [DOI] [PubMed] [Google Scholar]
  4. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hinkle N. F., Miller R. V. pMG7-mediated restriction of Pseudomonas aeruginosa phage DNAs is determined by a class II restriction endonuclease. Plasmid. 1979 Jul;2(3):387–393. doi: 10.1016/0147-619x(79)90022-2. [DOI] [PubMed] [Google Scholar]
  6. Holloway B. W., Krishnapillai V., Morgan A. F. Chromosomal genetics of Pseudomonas. Microbiol Rev. 1979 Mar;43(1):73–102. doi: 10.1128/mr.43.1.73-102.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Lehrbach P. R., Dirckze C. D., Lee B. T. A mutant of Pseudomonas aeruginosa deficient in an ATP-dependent deoxyribonuclease. J Gen Microbiol. 1980 Oct;120(2):377–384. doi: 10.1099/00221287-120-2-377. [DOI] [PubMed] [Google Scholar]
  9. Loutit J. S. Investigation of the mating system of Pseudomonas aeruginosa strain 1. V. The effect of N-methyl-N'-nitro-N-nitrosoguanidine on a donor strain. Genet Res. 1969 Oct;14(2):103–109. doi: 10.1017/s0016672300001932. [DOI] [PubMed] [Google Scholar]
  10. Mercer A. A., Loutit J. S. Transformation and transfection of Pseudomonas aeruginosa: effects of metal ions. J Bacteriol. 1979 Oct;140(1):37–42. doi: 10.1128/jb.140.1.37-42.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Miller R. V., Clark A. J. Purification and properties of two deoxyribonucleases of Pseudomonas aeruginosa. J Bacteriol. 1976 Aug;127(2):794–802. doi: 10.1128/jb.127.2.794-802.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Miller R. V., Pemberton J. M., Clark A. J. Prophage F116: evidence for extrachromosomal location in Pseudomonas aeruginosa strain PAO. J Virol. 1977 Jun;22(3):844–847. doi: 10.1128/jvi.22.3.844-847.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Miller R. V., Pemberton J. M., Richards K. E. F116, D3 and G101: temperate bacteriophages of Pseudomonas aeruginosa. Virology. 1974 Jun;59(2):566–569. doi: 10.1016/0042-6822(74)90466-8. [DOI] [PubMed] [Google Scholar]
  15. Oishi M., Cosloy S. D. The genetic and biochemical basis of the transformability of Escherichia coli K12. Biochem Biophys Res Commun. 1972 Dec 18;49(6):1568–1572. doi: 10.1016/0006-291x(72)90520-7. [DOI] [PubMed] [Google Scholar]
  16. PEARCE L. E., LOUTIT J. S. BIOCHEMICAL AND GENETIC GROUPING OF ISOLEUCINE-VALINE MUTANTS OF PSEUDOMONAS AERUGINOSA. J Bacteriol. 1965 Jan;89:58–63. doi: 10.1128/jb.89.1.58-63.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pemberton J. M., Clark A. J. Detection and characterization of plasmids in Pseudomonas aeruginosa strain PAO. J Bacteriol. 1973 Apr;114(1):424–433. doi: 10.1128/jb.114.1.424-433.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Scurlock T. R., Miller R. V. PaeExo IX: a unique deoxyribonuclease from Pseudomonas aeruginosa active in the presence of EDTA. Nucleic Acids Res. 1979 Sep 11;7(1):167–177. doi: 10.1093/nar/7.1.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stanisich V. A., Bennett P. M. The properties of hybrids formed between the P-group plasmid RP1 and various plasmids from Pseudomonas aeruginosa. Mol Gen Genet. 1976 Dec 8;149(2):217–223. doi: 10.1007/BF00332892. [DOI] [PubMed] [Google Scholar]
  20. Vogt V. M. Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae. Eur J Biochem. 1973 Feb 15;33(1):192–200. doi: 10.1111/j.1432-1033.1973.tb02669.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES