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. 1982 Sep;151(3):1473–1484. doi: 10.1128/jb.151.3.1473-1484.1982

Soluble aldehyde dehydrogenase and metabolism of aldehydes by soybean bacteroids.

J B Peterson, T A LaRue
PMCID: PMC220429  PMID: 7202001

Abstract

A soluble aldehyde dehydrogenase (EC 1.2.1.3) was partially purified from Rhizobium japonicum bacteroids and from free-living R. japonicum 61A76. The enzyme was activated by NAD+, NADH, and dithiothreitol, and it reduced NAD(P)+. Acetaldehyde, propionaldehyde, butyraldehyde, benzaldehyde, and succinic semialdehyde were substrates. The Km for straight-chain aldehydes decreased with increasing carbon chain length. The aldehyde dehydrogenase was inhibited by 6-cyanopurine, but not by metronidazole. These compounds inhibited acetylene reduction, but not respiration, by isolated bacteroids.

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Selected References

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