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. 1982 Sep;151(3):1605–1607. doi: 10.1128/jb.151.3.1605-1607.1982

A plasmid cloning vehicle for Haemophilus influenzae and Escherichia coli.

D McCarthy, N L Clayton, J K Setlow
PMCID: PMC220445  PMID: 6286603

Abstract

A new plasmid cloning vehicle (pDM2) was used to introduce a library of Haemophilus influenzae chromosomal fragments into H. influenzae. Transformants of the highly recombination-defective rec-1 mutant were more likely to contain exclusively recombinant plasmids after exposure to ligated DNA mixtures than was the wild type. pDM2 could replicate in Escherichia coli K-12.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albritton W. L., Bendler J. W., Setlow J. K. Plasmid transformation in Haemophilus influenzae. J Bacteriol. 1981 Feb;145(2):1099–1101. doi: 10.1128/jb.145.2.1099-1101.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barnhart B. J., Cox S. H. Radiation-sensitive and radiation-resistant mutants of Haemophilus influenzae. J Bacteriol. 1968 Jul;96(1):280–282. doi: 10.1128/jb.96.1.280-282.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boling M. E., Allison D. P., Setlow J. K. Bacteriophage of Haemophilus influenzae. 3. Morphology, DNA homology, and immunity properties of HPlcl, S2, and the defective bacteriophage from strain Rd. J Virol. 1973 Apr;11(4):585–591. doi: 10.1128/jvi.11.4.585-591.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brunton J., Bennett P., Grinsted J. Molecular nature of a plasmid specifying beta-lactamase production in Haemophilus ducreyi. J Bacteriol. 1981 Dec;148(3):788–795. doi: 10.1128/jb.148.3.788-795.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. De Graaff J., Elwell L. P., Falkow S. Molecular nature of two beta-lactamase-specifying plasmids isolated from Haemophilus influenzae type b. J Bacteriol. 1976 Apr;126(1):439–446. doi: 10.1128/jb.126.1.439-446.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dugaiczyk A., Boyer H. W., Goodman H. M. Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structures. J Mol Biol. 1975 Jul 25;96(1):171–184. doi: 10.1016/0022-2836(75)90189-8. [DOI] [PubMed] [Google Scholar]
  7. Setlow J. K., Boling M. E., Beattie K. L., Kimball R. F. A complex of recombination and repair genes in Haemophilus influenzae. J Mol Biol. 1972 Jul 21;68(2):361–378. doi: 10.1016/0022-2836(72)90218-5. [DOI] [PubMed] [Google Scholar]
  8. Setlow J. K., Brown D. C., Boling M. E., Mattingly A., Gordon M. P. Repair of deoxyribonucleic acid in Haemophilus influenzae. I. X-ray sensitivity of ultraviolet-sensitive mutants and their behavior as hosts to ultraviolet-irradiated bacteriophage and transforming deoxyribonucleic acid. J Bacteriol. 1968 Feb;95(2):546–558. doi: 10.1128/jb.95.2.546-558.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Setlow J. K., Notani N. K., McCarthy D., Clayton N. L. Transformation of Haemophilus influenzae by plasmid RSF0885 containing a cloned segment of chromosomal deoxyribonucleic acid. J Bacteriol. 1981 Dec;148(3):804–811. doi: 10.1128/jb.148.3.804-811.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Steinhart W. L., Herriott R. M. Fate of recipient deoxyribonucleic acid during transformation in Haemophilus influenzae. J Bacteriol. 1968 Nov;96(5):1718–1724. doi: 10.1128/jb.96.5.1718-1724.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

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