Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1982 Sep;151(3):1608–1611. doi: 10.1128/jb.151.3.1608-1611.1982

Overproduction of cis-Vaccenic Acid and Altered Temperature Control of Fatty Acid Synthesis in a Mutant of Escherichia coli

Diego de Mendoza 1, Jeffrey L Garwin 1,†, John E Cronan Jr 1
PMCID: PMC220446  PMID: 7050094

Abstract

A mutant of Escherichia coli has been characterized which overproduces cis-vaccenic acid in the temperature range of 30 to 42°C. The mutational lesion acts within the fatty acid biosynthetic pathway rather than at the level of fatty acid incorporation into phospholipid.

Full text

PDF
1608

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baldassare J. J., Rhinehart K. B., Silbert D. F. Modification of membrane lipid: physical properties in relation to fatty acid structure. Biochemistry. 1976 Jul 13;15(14):2986–2994. doi: 10.1021/bi00659a008. [DOI] [PubMed] [Google Scholar]
  2. Clark D., Cronan J. E., Jr Escherichia coli mutants with altered control of alcohol dehydrogenase and nitrate reductase. J Bacteriol. 1980 Jan;141(1):177–183. doi: 10.1128/jb.141.1.177-183.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cronan J. E., Jr, Gelmann E. P. Physical properties of membrane lipids: biological relevance and regulation. Bacteriol Rev. 1975 Sep;39(3):232–256. doi: 10.1128/br.39.3.232-256.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cronan J. E., Jr Thermal regulation of the membrane lipid composition of Escherichia coli. Evidence for the direct control of fatty acid synthesis. J Biol Chem. 1975 Sep 10;250(17):7074–7077. [PubMed] [Google Scholar]
  5. Cronan J. E., Jr, Weisberg L. J., Allen R. G. Regulation of membrane lipid synthesis in Escherichia coli. Accumulation of free fatty acids of abnormal length during inhibition of phospholipid synthesis. J Biol Chem. 1975 Aug 10;250(15):5835–5840. [PubMed] [Google Scholar]
  6. Garwin J. L., Cronan J. E., Jr Thermal modulation of fatty acid synthesis in Escherichia coli does not involve de novo enzyme synthesis. J Bacteriol. 1980 Mar;141(3):1457–1459. doi: 10.1128/jb.141.3.1457-1459.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Garwin J. L., Klages A. L., Cronan J. E., Jr Beta-ketoacyl-acyl carrier protein synthase II of Escherichia coli. Evidence for function in the thermal regulation of fatty acid synthesis. J Biol Chem. 1980 Apr 25;255(8):3263–3265. [PubMed] [Google Scholar]
  8. Garwin J. L., Klages A. L., Cronan J. E., Jr Structural, enzymatic, and genetic studies of beta-ketoacyl-acyl carrier protein synthases I and II of Escherichia coli. J Biol Chem. 1980 Dec 25;255(24):11949–11956. [PubMed] [Google Scholar]
  9. Gelmann E. P., Cronan J. E., Jr Mutant of Escherichia coli deficient in the synthesis of cis-vaccenic acid. J Bacteriol. 1972 Oct;112(1):381–387. doi: 10.1128/jb.112.1.381-387.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Goelz S. E., Cronan J. E., Jr The positional distribution of fatty acids in Escherichia coli phospholipids is not regulated by sn-glycerol 3-phosphate levels. J Bacteriol. 1980 Oct;144(1):462–464. doi: 10.1128/jb.144.1.462-464.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Maloy S. R., Nunn W. D. Selection for loss of tetracycline resistance by Escherichia coli. J Bacteriol. 1981 Feb;145(2):1110–1111. doi: 10.1128/jb.145.2.1110-1111.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Marr A. G., Ingraham J. L. EFFECT OF TEMPERATURE ON THE COMPOSITION OF FATTY ACIDS IN ESCHERICHIA COLI. J Bacteriol. 1962 Dec;84(6):1260–1267. doi: 10.1128/jb.84.6.1260-1267.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nishihara M., Ishinaga M., Kato M., Kito M. Temperature-sensitive formation of the phospholipid molecular species in Escherichia coli membranes. Biochim Biophys Acta. 1976 Apr 22;431(1):54–61. [PubMed] [Google Scholar]
  14. Okuyama H., Yamada K., Kameyama Y., Ikezawa H., Akamatsu Y., Nojima S. Regulation of membrane lipid synthesis in Escherichia coli after shifts in temperature. Biochemistry. 1977 Jun 14;16(12):2668–2673. doi: 10.1021/bi00631a013. [DOI] [PubMed] [Google Scholar]
  15. Polacco M. L., Cronan J. E., Jr Mechanism of the apparent regulation of Escherichia coli unsaturated fatty acid synthesis by exogenous oleic acid. J Biol Chem. 1977 Aug 10;252(15):5488–5490. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES