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. 1982 Sep;151(3):1621–1623. doi: 10.1128/jb.151.3.1621-1623.1982

Succinate dehydrogenase mutant of Rhizobium meliloti.

A Gardiol, A Arias, C Cerveñansky, G Martínez-Drets
PMCID: PMC220449  PMID: 6125502

Abstract

A succinate dehydrogenase mutant strain of Rhizobium meliloti was isolated after nitrosoguanidine mutagenesis. It failed to grow on succinate, glutamate, acetate, pyruvate, or arabinose but grew on glucose, sucrose, fructose, and other carbohydrates. The mutant strain showed delayed nodulation of lucerne plants, and the nodules were white and ineffective. A spontaneous revertant strain of normal growth phenotype induced red and effective nodules.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arias A., Cerveńansky C., Gardiol A., Martínez-Drets G. Phosphoglucose isomerase mutant of Rhizobium meliloti. J Bacteriol. 1979 Jan;137(1):409–414. doi: 10.1128/jb.137.1.409-414.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bach M. K., Magee W. E., Burris R. H. Translocation of Photosynthetic Products to Soybean Nodules and Their Role in Nitrogen Fixation. Plant Physiol. 1958 Mar;33(2):118–124. doi: 10.1104/pp.33.2.118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bergersen F. J., Turner G. L., Gibson A. H., Dudman W. F. Nitrogenase activity and respiration of cultures of Rhizobium spp. with special reference to concentrations of dissolved oxygen. Biochim Biophys Acta. 1976 Aug 24;444(1):164–174. doi: 10.1016/0304-4165(76)90233-6. [DOI] [PubMed] [Google Scholar]
  4. Duncan M. J., Fraenkel D. G. alpha-Ketoglutarate dehydrogenase mutant of Rhizobium meliloti. J Bacteriol. 1979 Jan;137(1):415–419. doi: 10.1128/jb.137.1.415-419.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Edwards D. L., Belsole D. M., Guzik H. J., Unger B. W. Selection of succinic dehydrogenase mutants of Neurospora crassa. J Bacteriol. 1979 Feb;137(2):900–904. doi: 10.1128/jb.137.2.900-904.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Finan T. M., Wood J. M., Jordan D. C. Succinate transport in Rhizobium leguminosarum. J Bacteriol. 1981 Oct;148(1):193–202. doi: 10.1128/jb.148.1.193-202.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GRAHAM P. H. STUDIES ON THE UTILISATION OF CARBOHYDRATES AND KREBS CYCLE INTERMEDIATES BY RHIZOBIA, USING AN AGAR PLATE METHOD. Antonie Van Leeuwenhoek. 1964;30:68–72. doi: 10.1007/BF02046703. [DOI] [PubMed] [Google Scholar]
  8. Gardiol A., Arias A., Cerveñansky C., Gaggero C., Martínez-Drets G. Biochemical characterization of a fructokinase mutant of Rhizobium meliloti. J Bacteriol. 1980 Oct;144(1):12–16. doi: 10.1128/jb.144.1.12-16.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gibson A. H., Scowcroft W. R., Child J. J., Pagan J. D. Nitrogenase activity in cultured Rhizobium sp. strain 32H1: nutritional and physical considerations. Arch Microbiol. 1976 May 3;108(1):45–54. doi: 10.1007/BF00425092. [DOI] [PubMed] [Google Scholar]
  10. KATZNELSON H., ZAGALLO A. C. Metabolism of rhizobia in relation to effectiveness. Can J Microbiol. 1957 Oct;3(6):879–884. doi: 10.1139/m57-097. [DOI] [PubMed] [Google Scholar]
  11. Khouw B. T., McCurdy H. D. Tricarboxylic acid cycle enzymes and morphogenesis in Blastocladiella emersonii. J Bacteriol. 1969 Jul;99(1):197–205. doi: 10.1128/jb.99.1.197-205.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ronson C. W., Lyttleton P., Robertson J. G. C(4)-dicarboxylate transport mutants of Rhizobium trifolii form ineffective nodules on Trifolium repens. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4284–4288. doi: 10.1073/pnas.78.7.4284. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SRERE P. A., KOSICKI G. W. The purification of citrate-condensing enzyme. J Biol Chem. 1961 Oct;236:2557–2559. [PubMed] [Google Scholar]
  14. Stovall I., Cole M. Organic Acid Metabolism by Isolated Rhizobium japonicum Bacteroids. Plant Physiol. 1978 May;61(5):787–790. doi: 10.1104/pp.61.5.787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stumpf D. K., Burris R. H. A micromethod for the purification and quantification of organic acids of the tricarboxylic acid cycle in plant tissues. Anal Biochem. 1979 May;95(1):311–315. doi: 10.1016/0003-2697(79)90221-5. [DOI] [PubMed] [Google Scholar]

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