TABLE 1.
Temperature-sensitive maternal-effect sterility of meg-1 mutants
| % sterile (n)
|
|||
|---|---|---|---|
| Genotype | 15° | 20° | 25° |
| Maternal-effect sterility of meg-1 mutants | |||
| P0meg-1(vr10)/+ at 20° | |||
| F1meg-1(vr10) | 0 (5) | 0 (5) | 0 (4) |
| F2meg-1(vr10)a | 13 (379) | 1 (925) | 75 (734) |
| F1meg-1(vr10)/+ | 0 (8) | 0 (9) | 0 (5) |
| F2 +/+, meg-1(vr10)/+, and meg-1(vr10)a | 0 (619) | 0 (1344) | 0 (736) |
| P0meg-1(vr11)/+ at 20° | |||
| F1meg-1(vr11) | 0 (3) | 0 (4) | 0 (3) |
| F2meg-1(vr11)a | 8 (239) | 0 (461) | 45 (447) |
| F1meg-1(vr11)/+ | 0 (9) | 0 (7) | 0 (8) |
| F2 +/+, meg-1(vr11)/+, and meg-1(vr11)a | 0 (855) | 0 (882) | 0 (1011) |
| Zygotic meg-1 does not rescue sterility | |||
| meg-1(vr10) | ND | ND | 49 (220) |
| meg-1(vr10) × N2 male | ND | ND | 49 (384) |
| meg-1(vr11) | ND | ND | 47 (187) |
| meg-1(vr11) × N2 male | ND | ND | 30 (279) |
Animals were considered sterile if they displayed an “empty uterus” phenotype when observed under the dissecting microscope. F1's and F2's were raised at the indicated temperature. ND, not determined.
a
These F2 progeny are derived from the F1 mothers from the previous row.