Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1982 Oct;152(1):63–71. doi: 10.1128/jb.152.1.63-71.1982

Genetic and physical map of a P1 miniplasmid.

S Austin, F Hart, A Abeles, N Sternberg
PMCID: PMC221375  PMID: 6749822

Abstract

The prophage form of bacteriophage P1 is a unit-copy plasmid which is maintained with great fidelity in its Escherichia coli host. The plasmid maintenance functions of P1 are clustered in one region of the genome. An 11.5-kilobase fragment from this region has been cloned into a lambda delta att vector and promotes stable unit-copy plasmid maintenance. The properties of the lambda vector facilitated the isolation of deletion mutants affecting the P1 DNA. Twenty-eight deletion mutants were isolated, and their lesions were mapped by physical techniques. The genetic properties of the mutants with respect to plasmid replication, stability of plasmid maintenance, and ability to exert incompatibility effects against P1 and P7 plasmids were determined. These properties, along with those of several subfragments of the P1 insert cloned into high-copy-number plasmid vectors, allow the construction of an unambiguous genetic and physical map of the maintenance functions. A region of less than 3 kilobases, the rep region, is essential for plasmid replication and contains the incA incompatibility determinant within an 800-base-pair segment. Immediately adjacent to rep is a second region of approximately 3 kilobases which is required for stable plasmid maintenance, but not replication. This region, par, contains a second incompatibility element incB which is approximately 1 kilobase in size. The par region appears to specify equipartition of plasmid copies to daughter cells during cell division.

Full text

PDF
63

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appleyard R K. Segregation of New Lysogenic Types during Growth of a Doubly Lysogenic Strain Derived from Escherichia Coli K12. Genetics. 1954 Jul;39(4):440–452. doi: 10.1093/genetics/39.4.440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beckingham K. A plasmid cloning vector for Kpnl-cleaved DNA. Plasmid. 1980 Nov;4(3):354–356. doi: 10.1016/0147-619x(80)90074-8. [DOI] [PubMed] [Google Scholar]
  3. Bolivar F. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules. Gene. 1978 Oct;4(2):121–136. doi: 10.1016/0378-1119(78)90025-2. [DOI] [PubMed] [Google Scholar]
  4. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed] [Google Scholar]
  5. Bächi B., Arber W. Physical mapping of BglII, BamHI, EcoRI, HindIII and PstI restriction fragments of bacteriophage P1 DNA. Mol Gen Genet. 1977 Jun 24;153(3):311–324. doi: 10.1007/BF00431596. [DOI] [PubMed] [Google Scholar]
  6. Cowan J. A., Scott J. R. Incompatibility among group Y plasmids. Plasmid. 1981 Sep;6(2):202–221. doi: 10.1016/0147-619x(81)90067-6. [DOI] [PubMed] [Google Scholar]
  7. Dagert M., Ehrlich S. D. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene. 1979 May;6(1):23–28. doi: 10.1016/0378-1119(79)90082-9. [DOI] [PubMed] [Google Scholar]
  8. Daniels D. L., de Wet J. R., Blattner F. R. New map of bacteriophage lambda DNA. J Virol. 1980 Jan;33(1):390–400. doi: 10.1128/jvi.33.1.390-400.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dennert G., Henning U. Tyrosine-incorporating amber suppressors in Escherichia coli K12. J Mol Biol. 1968 Apr 14;33(1):327–329. doi: 10.1016/0022-2836(68)90300-8. [DOI] [PubMed] [Google Scholar]
  10. Enquist L. W., Weisberg R. A. The red plaque test: a rapid method for identification of excision defective variants of bacteriophage lambda. Virology. 1976 Jul 1;72(1):147–153. doi: 10.1016/0042-6822(76)90319-6. [DOI] [PubMed] [Google Scholar]
  11. Gottesman M. E., Yarmolinsky M. B. Integration-negative mutants of bacteriophage lambda. J Mol Biol. 1968 Feb 14;31(3):487–505. doi: 10.1016/0022-2836(68)90423-3. [DOI] [PubMed] [Google Scholar]
  12. Hall M. N., Silhavy T. J. Genetic analysis of the ompB locus in Escherichia coli K-12. J Mol Biol. 1981 Sep 5;151(1):1–15. doi: 10.1016/0022-2836(81)90218-7. [DOI] [PubMed] [Google Scholar]
  13. Hedges R. W., Jacob A. E., Barth P. T., Grinter N. J. Compatibility properties of P1 and PhiAMP prophages. Mol Gen Genet. 1975 Dec 1;141(3):263–267. doi: 10.1007/BF00341804. [DOI] [PubMed] [Google Scholar]
  14. Holmes D. S., Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981 Jun;114(1):193–197. doi: 10.1016/0003-2697(81)90473-5. [DOI] [PubMed] [Google Scholar]
  15. Ikeda H., Tomizawa J. Prophage P1, and extrachromosomal replication unit. Cold Spring Harb Symp Quant Biol. 1968;33:791–798. doi: 10.1101/sqb.1968.033.01.091. [DOI] [PubMed] [Google Scholar]
  16. Ishii K., Hashimoto-Gotoh T., Matsubara K. Random replication and random assortment model for plasmid incompatibility in bacteria. Plasmid. 1978 Sep;1(4):435–445. doi: 10.1016/0147-619x(78)90002-1. [DOI] [PubMed] [Google Scholar]
  17. KONDO E., MITSUHASHI S. DRUG RESISTANCE OF ENTERIC BACTERIA. IV. ACTIVE TRANSDUCING BACTERIOPHAGE P1 CM PRODUCED BY THE COMBINATION OF R FACTOR WITH BACTERIOPHAGE P1. J Bacteriol. 1964 Nov;88:1266–1276. doi: 10.1128/jb.88.5.1266-1276.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kelley W. S. Mapping of the polA locus of Escherichia coli K12: genetic fine structure of the cistron. Genetics. 1980 May;95(1):15–38. doi: 10.1093/genetics/95.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nordström K., Molin S., Aagaard-Hansen H. Partitioning of plasmid R1 in Escherichia coli. II. Incompatibility properties of the partitioning system. Plasmid. 1980 Nov;4(3):332–339. doi: 10.1016/0147-619x(80)90071-2. [DOI] [PubMed] [Google Scholar]
  20. Novick R. P. Extrachromosomal inheritance in bacteria. Bacteriol Rev. 1969 Jun;33(2):210–263. doi: 10.1128/br.33.2.210-263.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Novick R. P., Hoppensteadt F. C. On plasmid incompatibility. Plasmid. 1978 Sep;1(4):421–434. doi: 10.1016/0147-619x(78)90001-x. [DOI] [PubMed] [Google Scholar]
  22. Prentki P., Chandler M., Caro L. Replication of prophage P1 during the cell cycle of Escherichia coli. Mol Gen Genet. 1977 Mar 28;152(1):71–76. doi: 10.1007/BF00264942. [DOI] [PubMed] [Google Scholar]
  23. Som T., Sternberg N., Austin S. A nonsense mutation in bacteriophage P1 eliminates the synthesis of a protein required for normal plasmid maintenance. Plasmid. 1981 Mar;5(2):150–160. doi: 10.1016/0147-619x(81)90016-0. [DOI] [PubMed] [Google Scholar]
  24. Sternberg N. A characterization of bacteriophage P1 DNA fragments cloned in a lambda vector. Virology. 1979 Jul 15;96(1):129–142. doi: 10.1016/0042-6822(79)90179-x. [DOI] [PubMed] [Google Scholar]
  25. Sternberg N. A genetic analysis of bacteriophage lambda head assembly. Virology. 1976 Jun;71(2):568–582. doi: 10.1016/0042-6822(76)90382-2. [DOI] [PubMed] [Google Scholar]
  26. Sternberg N., Austin S. The maintenance of the P1 plasmid prophage. Plasmid. 1981 Jan;5(1):20–31. doi: 10.1016/0147-619x(81)90075-5. [DOI] [PubMed] [Google Scholar]
  27. Sternberg N., Hamilton D., Enquist L., Weisberg R. A simple technique for the isolation of deletion mutants of phage lambda. Gene. 1979 Dec;8(1):35–51. doi: 10.1016/0378-1119(79)90006-4. [DOI] [PubMed] [Google Scholar]
  28. Sternberg N., Powers M., Yarmolinsky M., Austin S. Group Y incompatibility and copy control of P1 prophage. Plasmid. 1981 Mar;5(2):138–149. doi: 10.1016/0147-619x(81)90015-9. [DOI] [PubMed] [Google Scholar]
  29. Sternberg N., Weisberg R. Packaging of prophage and host DNA by coliphage lambda. Nature. 1975 Jul 10;256(5513):97–103. doi: 10.1038/256097a0. [DOI] [PubMed] [Google Scholar]
  30. Szybalski E. H., Szybalski W. A comprehensive molecular map of bacteriophage lambda. Gene. 1979 Nov;7(3-4):217–270. doi: 10.1016/0378-1119(79)90047-7. [DOI] [PubMed] [Google Scholar]
  31. Tanaka T., Weisblum B. Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid. J Bacteriol. 1975 Jan;121(1):354–362. doi: 10.1128/jb.121.1.354-362.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Walker D. H., Jr, Walker J. T. Genetic studies of coliphage P1. III. Extended genetic map. J Virol. 1976 Oct;20(1):177–187. doi: 10.1128/jvi.20.1.177-187.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Wandersman C., Yarmolinsky M. Bipartite control of immunity conferred by the related heteroimmune plasmid prophages, P1 and P7 (formerly phi Amp). Virology. 1977 Mar;77(1):386–400. doi: 10.1016/0042-6822(77)90435-4. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES