Figure 2.

The antimicrobial peptide gomesin showed in vitro cytotoxic effect on B16F10-Nex2 murine tumor cells, in a dose-, time-, and structure-dependent fashion. (A) Tumor cells were incubated for 12 hours with 20 µM gomesin (Gm), its d-enantiomer (d-Gm), monocyclic {[Ser^2,15]-Gm}, linear {[Ser^2,6,11,15]-Gm}, forms, and with 10 µM analogues with amino acid substitutions disrupting the hydrophobic face of the peptide, [Ser⁵]-Gm, [Ser¹²]-Gm, and [Ser^5,12]-Gm. (B) Cells were incubated with different concentrations of Gm (1, 5, 10, and 20 µM) for 15 minutes up to 5 hours. Cells were alternatively incubated with Gm for 5 hours, washed to eliminate the peptide, and incubated with fresh medium for 24 hours (5/24 h). Cell viability was determined by counting cells in presence of Trypan blue. Data are a representative experiment of a triplicate set. Bars represent means and standard deviations (SD). *P < .05 and #P < .01 relative to the control.