Abstract
Dynamic processes on the membrane of the procaryotic cell Acholeplasma laidlawii have been studied by means of immunoelectron microscopy. Colloidal gold-labeled anti-A. laidlawii antibodies were used as electron-dense markers. This method allowed the demonstration of temperature-dependent lateral mobility of membrane-bound immunoglobulins. By using two different sizes of gold grains to differentiate cells from two different cell populations, virus-induced fusion of procaryotic cells could be shown for the first time.
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- Fodor K., Alföldi L. Fusion of protoplasts of Bacillus megaterium. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2147–2150. doi: 10.1073/pnas.73.6.2147. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Geoghegan W. D., Ackerman G. A. Adsorption of horseradish peroxidase, ovomucoid and anti-immunoglobulin to colloidal gold for the indirect detection of concanavalin A, wheat germ agglutinin and goat anti-human immunoglobulin G on cell surfaces at the electron microscopic level: a new method, theory and application. J Histochem Cytochem. 1977 Nov;25(11):1187–1200. doi: 10.1177/25.11.21217. [DOI] [PubMed] [Google Scholar]
- Gething M. J., White J. M., Waterfield M. D. Purification of the fusion protein of Sendai virus: analysis of the NH2-terminal sequence generated during precursor activation. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2737–2740. doi: 10.1073/pnas.75.6.2737. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haberer K., Klotz G., Maniloff J., Kleinschmidt A. K. Structural and biological properties of mycoplasmavirus MVL3: an unusual virus-procaryote interaction. J Virol. 1979 Oct;32(1):268–275. doi: 10.1128/jvi.32.1.268-275.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haberer K., Maniloff J. Adsorption of the tailed mycoplasma virus L3 to cell membranes. J Virol. 1982 Feb;41(2):501–507. doi: 10.1128/jvi.41.2.501-507.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haberer K., Maniloff J., Gerling D. Adsorption, capping, and release of a complex bacteriophage by mycoplasma cells. J Virol. 1980 Oct;36(1):264–270. doi: 10.1128/jvi.36.1.264-270.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haberer K., Pfisterer M. Altered surface structure of Acholeplasma laidlawii induced by mycoplasmavirus L3 infection. Eur J Cell Biol. 1981 Aug;25(1):10–12. [PubMed] [Google Scholar]
- Maniloff J., Chaudhuri U. Gliding mycoplasmas are inhibited by cytochalasin B and contain a polymerizable protein fraction. J Supramol Struct. 1979;12(3):299–304. doi: 10.1002/jss.400120303. [DOI] [PubMed] [Google Scholar]
- Melchior D. L., Steim J. M. Control of fatty acid composition of Acholeplasma laidlawii membranes. Biochim Biophys Acta. 1977 Apr 1;466(1):148–159. doi: 10.1016/0005-2736(77)90215-2. [DOI] [PubMed] [Google Scholar]
- Neimark H. C. Extraction of an actin-like protein from the prokaryote Mycoplasma pneumoniae. Proc Natl Acad Sci U S A. 1977 Sep;74(9):4041–4045. doi: 10.1073/pnas.74.9.4041. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rothman J. E., Engelman D. M. Molecular mechanism for the interaction of phospholipid with cholesterol. Nat New Biol. 1972 May 10;237(71):42–44. doi: 10.1038/newbio237042a0. [DOI] [PubMed] [Google Scholar]
- Schaeffer P., Cami B., Hotchkiss R. D. Fusion of bacterial protoplasts. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2151–2155. doi: 10.1073/pnas.73.6.2151. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schiefer H. G., Krauss H., Brunner H., Gerhardt U. Ultrastructural visualization of anionic sites on mycoplasma membranes by polycationic ferritin. J Bacteriol. 1976 Jul;127(1):461–468. doi: 10.1128/jb.127.1.461-468.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Steim J. M., Tourtellotte M. E., Reinert J. C., McElhaney R. N., Rader R. L. Calorimetric evidence for the liquid-crystalline state of lipids in a biomembrane. Proc Natl Acad Sci U S A. 1969 May;63(1):104–109. doi: 10.1073/pnas.63.1.104. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Séchaud J., Kellenberger E. Electron microscopy of DNA-containing plasms. IV. Glutaraldehyde-uranyl acetate fixation of virus-infected bacteria for thin sectioning. J Ultrastruct Res. 1972 Jun;39(5):598–607. doi: 10.1016/s0022-5320(72)90124-4. [DOI] [PubMed] [Google Scholar]
- Séchaud J., Kellenberger E. Electron microscopy of DNA-containing plasms. IV. Glutaraldehyde-uranyl acetate fixation of virus-infected bacteria for thin sectioning. J Ultrastruct Res. 1972 Jun;39(5):598–607. doi: 10.1016/s0022-5320(72)90124-4. [DOI] [PubMed] [Google Scholar]
- Wallace B. A., Engelman D. M. The planar distributions of surface proteins and intramembrane particles in Acholeplasma laidlawii are differentially affected by the physical state of membrane lipids. Biochim Biophys Acta. 1978 Apr 20;508(3):431–449. doi: 10.1016/0005-2736(78)90090-1. [DOI] [PubMed] [Google Scholar]
- Wallace B. A., Richards F. M., Engelman D. M. The influence of lipid state on the planar distribution of membrane proteins in Acholeplasma laidlawii. J Mol Biol. 1976 Nov 5;107(3):255–269. doi: 10.1016/s0022-2836(76)80004-6. [DOI] [PubMed] [Google Scholar]




