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. 1975 Mar 1;65(3):275–291. doi: 10.1085/jgp.65.3.275

Metabolism of acetylcholine in the nervous system of Aplysia californica. II. Reginal localization and characterization of choline uptake

PMCID: PMC2214874  PMID: 1117283

Abstract

The choline required for synthesis of acetylcholine is derived exogenously by Aplysia ganglia. Under physiological conditions choline was taken up primarlily by neuropile and nerves and not by cholinergic cell bodies. In addition, compared with their contents of choline acetyltransferase, those components of nervous tissue which contain nerve terminals and axons synthesized acetylcholine far more efficiently. Choline was accumulated by high and low affinity uptake processes; the high affinity process appeared to be characteristic of cholinergic nuerons (Swartz, J. H., M. L. Eisenstadt, and H. Cedar.1975. J. Gen. Physiol. 65:255). The two uptake processes were similarly affected by temperature with a Q10 of 2.8. Both were dependent on a variety of ions in a complicated manner. High affinity uptake seemed to be more dependent on Na+, showed greater inhibition by ouabain, and was selectively inhibited by oxotremorine. We found that the functional state of neurons did not alter uptake of radioactive choline by either process, nor did it change the conversion to radioactive acetylcholine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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