Abstract
In the honey bee drone, the decrease in sensitivity to light of a retinula cell exposed to background illumination was found to be accurately reflected by the difference in amplitude between the initial transient depolarization and the lowest steady depolarization evoked by the background light. It is shown that both the decrease in sensitivity to light and the accompanying drop in potential from the transient to the plateau can be prevented by injecting EGTA intracellularly. A decrease in duration and amplitude of responses to short test flashes such as observed immediately after illumination was found to occur too when Ca or Na, but not K, Li, or Mg injected into dark-adapted retinula cells. Injection of EGTA into a retinula cell maintained a steady state of light adaptation, was found to cause an increase in amplitude and duration of the response to a short test flash, thus producing the effects of dark adaptation. It is suggested that, in the retina of the honey bee drone, an increase in intracellular calcium concentration plays a central role in light adaptation and that an increase in intracellular sodium concentration, resulting from the influx of sodium ions during the responses to light, could lead to this increase in intracellular free calcium.
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Selected References
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- Bader C., Bertrand D., Bertrand G., Perrelet A. A pressure device for intracellular injection. Experientia. 1974 Nov 15;30(11):1366–1367. doi: 10.1007/BF01945232. [DOI] [PubMed] [Google Scholar]
- Baumann F., Hadjilazaro B. A depolarizing aftereffect of intense light in the drone visual receptor. Vision Res. 1972 Jan;12(1):17–31. doi: 10.1016/0042-6989(72)90134-4. [DOI] [PubMed] [Google Scholar]
- Baumann F. Influence of light adaptation and intracellular injection of sodium on the receptor potential of drone retinula cells. J Physiol. 1972 Oct;226(2):114P–115P. [PubMed] [Google Scholar]
- Baumann F., Mauro A. Effect of hypoxia on the change in membrane conductance evoked by illumination in arthropod photoreceptors. Nat New Biol. 1973 Aug 1;244(135):146–148. doi: 10.1038/newbio244146b0. [DOI] [PubMed] [Google Scholar]
- Baumann F., Perrelet A., Fulpius B. Etude fonctionnelle et morphologique de la cellule rétinienne du faux-bourdon au cours de l'adaptation à la lumière et à l'obscurité. Helv Physiol Pharmacol Acta. 1967;25(2):CR163–CR164. [PubMed] [Google Scholar]
- Baumann F. Slow and spike potentials recorded from retinula cells of the honeybee drone in response to light. J Gen Physiol. 1968 Dec;52(6):855–875. doi: 10.1085/jgp.52.6.855. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Blaustein M. P. The interrelationship between sodium and calcium fluxes across cell membranes. Rev Physiol Biochem Pharmacol. 1974;70:33–82. doi: 10.1007/BFb0034293. [DOI] [PubMed] [Google Scholar]
- Brown H. M., Hagiwara S., Koike H., Meech R. M. Membrane properties of a barnacle photoreceptor examined by the voltage clamp technique. J Physiol. 1970 Jun;208(2):385–413. doi: 10.1113/jphysiol.1970.sp009127. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brown J. E., Blinks J. R. Changes in intracellular free calcium concentration during illumination of invertebrate photoreceptors. Detection with aequorin. J Gen Physiol. 1974 Dec;64(6):643–665. doi: 10.1085/jgp.64.6.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Carafoli E., Tiozzo R., Lugli G., Crovetti F., Kratzing C. The release of calcium from heart mitochondria by sodium. J Mol Cell Cardiol. 1974 Aug;6(4):361–371. doi: 10.1016/0022-2828(74)90077-7. [DOI] [PubMed] [Google Scholar]
- Dowling J. E., Ripps H. Adaptation in skate photoreceptors. J Gen Physiol. 1972 Dec;60(6):698–719. doi: 10.1085/jgp.60.6.698. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fein A., DeVoe R. D. Adaptation in the ventral eye of Limulus is functionally independent of the photochemical cycle, membrane potential, and membrane resistance. J Gen Physiol. 1973 Mar;61(3):273–289. doi: 10.1085/jgp.61.3.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fulpius B., Baumann F. Effects of sodium, potassium, and calcium ions on slow and spike potentials in single photoreceptor cells. J Gen Physiol. 1969 May;53(5):541–561. doi: 10.1085/jgp.53.5.541. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GOLDSMITH T. H. THE COURSE OF LIGHT AND DARK ADAPTATION IN THE COMPOUND EYE OF THE HONEY-BEE. Comp Biochem Physiol. 1963 Nov;10:227–237. doi: 10.1016/0010-406x(63)90036-7. [DOI] [PubMed] [Google Scholar]
- Lisman J. E., Brown J. E. Effects of intracellular injection of calcium buffers on light adaptation in Limulus ventral photoreceptors. J Gen Physiol. 1975 Oct;66(4):489–506. doi: 10.1085/jgp.66.4.489. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lisman J. E., Brown J. E. Light-induced changes of sensitivity in Limulus ventral photoreceptors. J Gen Physiol. 1975 Oct;66(4):473–488. doi: 10.1085/jgp.66.4.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lisman J. E., Brown J. E. The effects of intracellular iontophoretic injection of calcium and sodium ions on the light response of Limulus ventral photoreceptors. J Gen Physiol. 1972 Jun;59(6):701–719. doi: 10.1085/jgp.59.6.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Millecchia R., Mauro A. The ventral photoreceptor cells of Limulus. 3. A voltage-clamp study. J Gen Physiol. 1969 Sep;54(3):331–351. doi: 10.1085/jgp.54.3.331. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shaw S. R. Interreceptor coupling in ommatidia of drone honeybee and locust compound eyes. Vision Res. 1969 Sep;9(9):999–1029. doi: 10.1016/0042-6989(69)90044-3. [DOI] [PubMed] [Google Scholar]