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. 1981 Mar 1;77(3):317–334. doi: 10.1085/jgp.77.3.317

Rhodopsin kinetics in the cat retina

PMCID: PMC2215436  PMID: 7252477

Abstract

The bleaching and regeneration of rhodopsin in the living cat retina was studied by means of fundus reflectometry. Bleaching was effected by continuous light exposures of 1 min or 20 min, and the changes in retinal absorbance were measured at 29 wavelengths. For all of the conditions studied (fractional bleaches of from 65 to 100%), the regeneration of rhodopsin to its prebleach levels required greater than 60 min in darkness. After the 1-min exposures, the difference spectra recorded during the first 10 min of dark adaptation were dominated by photoproduct absorption, and rhodopsin regeneration kinetics were obscured by these intermediate processes. Extending the bleaching duration to 20 min gave the products of photolysis an opportunity to dissipate, and it was possible to follow the regenerative process over its full time-course. It was not possible, however, to fit these data with the simple exponential function predicted by first-order reaction kinetics. Other possible mechanisms were considered and are presented in the text. Nevertheless, the kinetics of regeneration compared favorably with the temporal changes in log sensitivity determined electrophysiologically by other investigators. Based on the bleaching curve for cat rhodopsin, the photosensitivity was determined and found to approximate closely the value obtained for human rhodopsin; i.e., the energy Ec required to bleach 1-e-1 of the available rhodopsin was 7.09 log scotopic troland-seconds (corrected for the optics of the cat eye), as compared with approximately 7.0 in man.

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Selected References

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  1. Alpern M., Pugh E. N., Jr The density and photosensitivity of human rhodopsin in the living retina. J Physiol. 1974 Mar;237(2):341–370. doi: 10.1113/jphysiol.1974.sp010485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Alpern M. Rhodopsin kinetics in the human eye. J Physiol. 1971 Sep;217(2):447–471. doi: 10.1113/jphysiol.1971.sp009580. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BARLOW H. B., FITZHUGH R., KUFFLER S. W. Dark adaptation, absolute threshold and Purkinje shift in single units of the cat's retina. J Physiol. 1957 Aug 6;137(3):327–337. doi: 10.1113/jphysiol.1957.sp005816. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baumann C. Regeneration of rhodopsin in the isolated retina of the frog (Rana esculenta). Vision Res. 1970 Aug;10(8):627–637. doi: 10.1016/0042-6989(70)90011-8. [DOI] [PubMed] [Google Scholar]
  5. Baumann C. Sehpurpurbleichung und Stäbchenfunktion in der isolierten Froschndtzhaut. 3. Die Dunkeladaptation des skotopischen Systems nach partieller Sehpurpurbleichung. Pflugers Arch Gesamte Physiol Menschen Tiere. 1967;298(1):70–81. [PubMed] [Google Scholar]
  6. Bonds A. B., Enroth-Cugell C. Recovery of cat retinal ganglion cell sensitivity following pigment bleaching. J Physiol. 1979 Oct;295:47–68. doi: 10.1113/jphysiol.1979.sp012954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bonds A. B., MacLeod D. I. The bleaching and regeneration of rhodopsin in the cat. J Physiol. 1974 Oct;242(1):237–253. doi: 10.1113/jphysiol.1974.sp010704. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Brin K. P., Ripps H. Rhodopsin photoproducts and rod sensitivity in the skate retina. J Gen Physiol. 1977 Jan;69(1):97–120. doi: 10.1085/jgp.69.1.97. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cone R. A., Brown P. K. Spontaneous regeneration of rhodopsin in the isolated rat retina. Nature. 1969 Mar 1;221(5183):818–820. doi: 10.1038/221818a0. [DOI] [PubMed] [Google Scholar]
  10. DARTNALL H. J. A. Visual pigment 467, a photosensitive pigment present in tench retinae. J Physiol. 1952 Mar;116(3):257–289. doi: 10.1113/jphysiol.1952.sp004705. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. DODT E., ELENIUS V. Change of threshold during dark adaptation measured with orange and blue light in cats and rabbits. Experientia. 1960 Jul 15;16:313–314. doi: 10.1007/BF02157769. [DOI] [PubMed] [Google Scholar]
  12. DOWLING J. E. Chemistry of visual adaptation in the rat. Nature. 1960 Oct 8;188:114–118. doi: 10.1038/188114a0. [DOI] [PubMed] [Google Scholar]
  13. DOWLING J. E. NEURAL AND PHOTOCHEMICAL MECHANISMS OF VISUAL ADAPTATION IN THE RAT. J Gen Physiol. 1963 Jul;46:1287–1301. doi: 10.1085/jgp.46.6.1287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Donner K. O., Reuter T. The dark-adaptation of single units in the frog's retina and its relation to the regeneration of rhodopsin. Vision Res. 1965 Dec;5(11):615–632. doi: 10.1016/0042-6989(65)90035-0. [DOI] [PubMed] [Google Scholar]
  15. Donner K. O., Reuter T. Visual adaptation of the rhodopsin rods in the frogs retina. J Physiol. 1968 Nov;199(1):59–87. doi: 10.1113/jphysiol.1968.sp008639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Dowling J. E., Ripps H. S-potentials in the skate retina. Intracellular recordings during light and dark adaptation. J Gen Physiol. 1971 Aug;58(2):163–189. doi: 10.1085/jgp.58.2.163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Dowling J. E., Ripps H. Visual adaptation in the retina of the skate. J Gen Physiol. 1970 Oct;56(4):491–520. doi: 10.1085/jgp.56.4.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Dowling J. E., Wald G. THE BIOLOGICAL FUNCTION OF VITAMIN A ACID. Proc Natl Acad Sci U S A. 1960 May;46(5):587–608. doi: 10.1073/pnas.46.5.587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Futterman S., Saari J. C., Blair S. Occurrence of a binding protein for 11-cis-retinal in retina. J Biol Chem. 1977 May 25;252(10):3267–3271. [PubMed] [Google Scholar]
  20. Grabowski S. R., Pak W. L. Intracellular recordings of rod responses during dark-adaptation. J Physiol. 1975 May;247(2):363–391. doi: 10.1113/jphysiol.1975.sp010936. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Granit R., Munsterhjelm A., Zewi M. The relation between concentration of visual purple and retinal sensitivity to light during dark adaptation. J Physiol. 1939 Jun 14;96(1):31–44. doi: 10.1113/jphysiol.1939.sp003755. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Green D. G., Dowling J. E., Siegel I. M., Ripps H. Retinal mechanisms of visual adaptation in the skate. J Gen Physiol. 1975 Apr;65(4):483–502. doi: 10.1085/jgp.65.4.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Hollins M., Alpern M. Dark adaptation and visual pigment regeneration in human cones. J Gen Physiol. 1973 Oct;62(4):430–447. doi: 10.1085/jgp.62.4.430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. LaMotte R. H., Brown J. L. Dark adaptation and spectral sensitivity in the cat. Vision Res. 1970 Aug;10(8):703–716. doi: 10.1016/0042-6989(70)90017-9. [DOI] [PubMed] [Google Scholar]
  25. Matsuura T. Rod late receptor potential and rhodopsin concentration of an isolated frog retina. Jpn J Physiol. 1975;25(2):123–133. doi: 10.2170/jjphysiol.25.123. [DOI] [PubMed] [Google Scholar]
  26. Pepperberg D. R., Brown P. K., Lurie M., Dowling J. E. Visual pigment and photoreceptor sensitivity in the isolated skate retina. J Gen Physiol. 1978 Apr;71(4):369–396. doi: 10.1085/jgp.71.4.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Perlman I. Kinetics of bleaching and regeneration of rhodopsin in abnormal (RCS) and normal albino rats in vivo. J Physiol. 1978 May;278:141–159. doi: 10.1113/jphysiol.1978.sp012297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. RUSHTON W. A. Dark-adaptation and the regeneration of rhodopsin. J Physiol. 1961 Apr;156:166–178. doi: 10.1113/jphysiol.1961.sp006666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. RUSHTON W. A. Rhodopsin measurement and dark-adaptation in a subject deficient in cone vision. J Physiol. 1961 Apr;156:193–205. doi: 10.1113/jphysiol.1961.sp006668. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. RUSHTON W. A. The rhodopsin density in the human rods. J Physiol. 1956 Oct 29;134(1):30–46. doi: 10.1113/jphysiol.1956.sp005623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Ripps H., Brin K. P., Weale R. A. Rhodopsin and visual threshold in retinitis pigmentosa. Invest Ophthalmol Vis Sci. 1978 Aug;17(8):735–745. [PubMed] [Google Scholar]
  32. Ripps H., Mehaffey L., 3rd, Siegel I. M., Ernst W., Kemp C. M. Flash photolysis of rhodopsin in the cat retina. J Gen Physiol. 1981 Mar;77(3):295–315. doi: 10.1085/jgp.77.3.295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Ripps H., Weale R. A. Flash bleaching of rhodopsin in the human retina. J Physiol. 1969 Jan;200(1):151–159. doi: 10.1113/jphysiol.1969.sp008686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Ripps H., Weale R. A. Rhodopsin regeneration in man. Nature. 1969 May 24;222(5195):775–777. doi: 10.1038/222775a0. [DOI] [PubMed] [Google Scholar]
  35. Steinberg R. H., Reid M., Lacy P. L. The distribution of rods and cones in the retina of the cat (Felis domesticus). J Comp Neurol. 1973 Mar 15;148(2):229–248. doi: 10.1002/cne.901480209. [DOI] [PubMed] [Google Scholar]
  36. Weale R. A. On an early stage of rhodopsin regeneration in man. Vision Res. 1967 Nov;7(11):819–827. doi: 10.1016/0042-6989(67)90002-8. [DOI] [PubMed] [Google Scholar]
  37. Witkovsky P., Gallin E., Hollyfield J. G., Ripps H., Bridges C. D. Photoreceptor thresholds and visual pigment levels in normal and vitamin A-deprived Xenopus tadpoles. J Neurophysiol. 1976 Nov;39(6):1272–1287. doi: 10.1152/jn.1976.39.6.1272. [DOI] [PubMed] [Google Scholar]

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