Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1982 Nov;152(2):919–923. doi: 10.1128/jb.152.2.919-923.1982

Escherichia coli polypeptide controlled by the lon (capR) ATP hydrolysis-dependent protease and possibly involved in cell division.

J M Schoemaker, G W Henderson, A Markovitz
PMCID: PMC221552  PMID: 6752125

Abstract

Mutation in the gene lon (capR) of Escherichia coli K-12 causes conditional inhibition of cell division. Two-dimensional gel electrophoresis was used to compare polypeptides from isogenic capR+ and capR strains. One polypeptide was present in the capR strain but absent in the wild-type strain, and it was proteolyzed when the pure capR+ protease was added to the capR extract. This polypeptide could only be detected in the capR strain when cell division was inhibited, and its synthesis was independent of the SOS response.

Full text

PDF
919

Images in this article

920Image
on p.920
921Image
on p.921

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADLER H. I., HARDIGREE A. A. ANALYSIS OF A GENE CONTROLLING CELL DIVISION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI. J Bacteriol. 1964 Mar;87:720–726. doi: 10.1128/jb.87.3.720-726.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beck E., Bremer E. Nucleotide sequence of the gene ompA coding the outer membrane protein II of Escherichia coli K-12. Nucleic Acids Res. 1980 Jul 11;8(13):3011–3027. doi: 10.1093/nar/8.13.3011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berg P. E., Gayda R., Avni H., Zehnbauer B., Markovitz A. Cloning of Escherichia coli DNA that controls cell division and capsular polysaccharide synthesis. Proc Natl Acad Sci U S A. 1976 Mar;73(3):697–701. doi: 10.1073/pnas.73.3.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bloch P. L., Phillips T. A., Neidhardt F. C. Protein identifications of O'Farrell two-dimensional gels: locations of 81 Escherichia coli proteins. J Bacteriol. 1980 Mar;141(3):1409–1420. doi: 10.1128/jb.141.3.1409-1420.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Buchanan C. E., Hua S. S., Avni H., Markovitz A. Transcriptional control of the calactose operon by the capR (lon) and capT genes. J Bacteriol. 1973 May;114(2):891–893. doi: 10.1128/jb.114.2.891-893.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Charette M. F., Henderson G. W., Markovitz A. ATP hydrolysis-dependent protease activity of the lon (capR) protein of Escherichia coli K-12. Proc Natl Acad Sci U S A. 1981 Aug;78(8):4728–4732. doi: 10.1073/pnas.78.8.4728. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Craig N. L., Roberts J. W. E. coli recA protein-directed cleavage of phage lambda repressor requires polynucleotide. Nature. 1980 Jan 3;283(5742):26–30. doi: 10.1038/283026a0. [DOI] [PubMed] [Google Scholar]
  8. Donch J., Greenberg J. Genetic analysis of lon mutants of strain K-12 of Escherichia coli. Mol Gen Genet. 1968;103(2):105–115. doi: 10.1007/BF00427138. [DOI] [PubMed] [Google Scholar]
  9. Gayda R. C., Avni H., Berg P. E., Markovitz A. Outer membrane protein a and other polypeptides regulate capsular polysaccharide synthesis in E. coli K-12. Mol Gen Genet. 1979 Oct 1;175(3):325–332. doi: 10.1007/BF00397232. [DOI] [PubMed] [Google Scholar]
  10. Gayda R. C., Markovitz A. Altered bacteriophage lambda expression in cell division mutants capR(lon) of Escherichia coli K-12. Mol Gen Genet. 1978 Feb 7;159(1):1–11. doi: 10.1007/BF00401741. [DOI] [PubMed] [Google Scholar]
  11. Gayda R. C., Yamamoto L. T., Markovitz A. Second-site mutations in capR (lon) strains of Escherichia coli K-12 that prevent radiation sensitivity and allow bacteriophage lambda to lysogenize. J Bacteriol. 1976 Sep;127(3):1208–1216. doi: 10.1128/jb.127.3.1208-1216.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gottesman S. Genetic control of the SOS system in E. coli. Cell. 1981 Jan;23(1):1–2. doi: 10.1016/0092-8674(81)90261-0. [DOI] [PubMed] [Google Scholar]
  13. Gottesman S., Gottesman M., Shaw J. E., Pearson M. L. Protein degradation in E. coli: the lon mutation and bacteriophage lambda N and cII protein stability. Cell. 1981 Apr;24(1):225–233. doi: 10.1016/0092-8674(81)90518-3. [DOI] [PubMed] [Google Scholar]
  14. Greenblatt J., Li J. The nusA gene protein of Escherichia coli. Its identification and a demonstration that it interacts with the gene N transcription anti-termination protein of bacteriophage lambda. J Mol Biol. 1981 Mar 25;147(1):11–23. doi: 10.1016/0022-2836(81)90076-0. [DOI] [PubMed] [Google Scholar]
  15. Gudas L. J., Mount D. W. Identification of the recA (tif) gene product of Escherichia coli. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5280–5284. doi: 10.1073/pnas.74.12.5280. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. HOWARD-FLANDERS P., SIMSON E., THERIOT L. A LOCUS THAT CONTROLS FILAMENT FORMATION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI K-12. Genetics. 1964 Feb;49:237–246. doi: 10.1093/genetics/49.2.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Huisman O., D'Ari R. An inducible DNA replication-cell division coupling mechanism in E. coli. Nature. 1981 Apr 30;290(5809):797–799. doi: 10.1038/290797a0. [DOI] [PubMed] [Google Scholar]
  18. Kowit J. D., Goldberg A. L. Intermediate steps in the degradation of a specific abnormal protein in Escherichia coli. J Biol Chem. 1977 Dec 10;252(23):8350–8357. [PubMed] [Google Scholar]
  19. Langley D., Guest J. R. Biochemical genetics of the alpha-keto acid dehydrogenase complexes of Escherichia coli K12: genetic characterization and regulatory properties of deletion mutants. J Gen Microbiol. 1978 May;106(1):103–117. doi: 10.1099/00221287-106-1-103. [DOI] [PubMed] [Google Scholar]
  20. Little J. W., Edmiston S. H., Pacelli L. Z., Mount D. W. Cleavage of the Escherichia coli lexA protein by the recA protease. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3225–3229. doi: 10.1073/pnas.77.6.3225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  22. O'Farrell P. Z., Goodman H. M., O'Farrell P. H. High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell. 1977 Dec;12(4):1133–1141. doi: 10.1016/0092-8674(77)90176-3. [DOI] [PubMed] [Google Scholar]
  23. Phillips T. A., Bloch P. L., Neidhardt F. C. Protein identifications on O'Farrell two-dimensional gels: locations of 55 additional Escherichia coli proteins. J Bacteriol. 1980 Dec;144(3):1024–1033. doi: 10.1128/jb.144.3.1024-1033.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Rice M. J. Supercontracting striated muscle in a vertebrate. Nature. 1973 May 25;243(5404):238–240. doi: 10.1038/243238a0. [DOI] [PubMed] [Google Scholar]
  25. Roberts J. W., Roberts C. W., Craig N. L., Phizicky E. M. Activity of the Escherichia coli recA-gene product. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):917–920. doi: 10.1101/sqb.1979.043.01.100. [DOI] [PubMed] [Google Scholar]
  26. Schoemaker J. M., Markovitz A. Identification of the gene lon (capR) product as a 94-kilodalton polypeptide by cloning and deletion analysis. J Bacteriol. 1981 Jul;147(1):46–56. doi: 10.1128/jb.147.1.46-56.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Shineberg B., Zipser D. The ion gene and degradation of beta-galactosidase nonsense fragments. J Bacteriol. 1973 Dec;116(3):1469–1471. doi: 10.1128/jb.116.3.1469-1471.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Takano T. Bacterial mutants defective in plasmid formation: requirement for the lon + allele. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1469–1473. doi: 10.1073/pnas.68.7.1469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Walker J. R., Ussery C. L., Allen J. S. Bacterial cell division regulation: lysogenization of conditional cell division lon - mutants of Escherichia coli by bacteriophage. J Bacteriol. 1973 Mar;113(3):1326–1332. doi: 10.1128/jb.113.3.1326-1332.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Zehnbauer B. A., Foley E. C., Henderson G. W., Markovitz A. Identification and purification of the Lon+ (capR+) gene product, a DNA-binding protein. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2043–2047. doi: 10.1073/pnas.78.4.2043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Zehnbauer B. A., Markovitz A. Cloning of gene lon (capR) of Escherichia coli K-12 and identification of polypeptides specified by the cloned deoxyribonucleic acid fragment. J Bacteriol. 1980 Aug;143(2):852–863. doi: 10.1128/jb.143.2.852-863.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES