Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1982 Nov;152(2):924–927. doi: 10.1128/jb.152.2.924-927.1982

Expression of the transposable lac operon Tn951 in Rhodopseudomonas sphaeroides.

F E Nano, S Kaplan
PMCID: PMC221553  PMID: 6290458

Abstract

The transposon Tn951 (lac) was introduced into the photosynthetic bacterium Rhodopseudomonas sphaeroides 2.4.1, which is normally Lac-, via the P-group plasmid RP1. beta-Galactosidase was produced constitutively in both chemotrophically and phototrophically grown cells, and the levels were found to be the same but low. Mutants were isolated, however, that were able to grow on lactose minimal medium and which expressed different levels of beta-galactosidase when grown chemotrophically or phototrophically. The beta-galactosidase levels found in all R. sphaeroides strains were much less than those found in Escherichia coli.

Full text

PDF
924

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aagaard J., Sistrom W. R. Control of synthesis of reaction center bacteriochlorophyll in photosynthetic bacteria. Photochem Photobiol. 1972 Feb;15(2):209–225. doi: 10.1111/j.1751-1097.1972.tb06240.x. [DOI] [PubMed] [Google Scholar]
  2. Baumberg S., Cornelis G., Panagiotakopoulos M., Roberts M. Expression of the lactose transposon Tn951 in Escherichia coli, Proteus and Pseudomonas. J Gen Microbiol. 1980 Jul;119(1):257–262. doi: 10.1099/00221287-119-1-257. [DOI] [PubMed] [Google Scholar]
  3. Cain B. D., Deal C. D., Fraley R. T., Kaplan S. In vivo intermembrane transfer of phospholipids in the photosynthetic bacterium Rhodopseudomonas sphaeroides. J Bacteriol. 1981 Mar;145(3):1154–1166. doi: 10.1128/jb.145.3.1154-1166.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cornelis G., Ghosal D., Saedler H. Tn951: a new transposon carrying a lactose operon. Mol Gen Genet. 1978 Apr 6;160(2):215–224. doi: 10.1007/BF00267484. [DOI] [PubMed] [Google Scholar]
  5. Cornelis G., Saedler H. Deletions and an inversion induced by a resident IS1 of the lactose transposon Tn951. Mol Gen Genet. 1980;178(2):367–374. doi: 10.1007/BF00270486. [DOI] [PubMed] [Google Scholar]
  6. Cornelis G. Transposition of Tn951 (Tnlac) and cointegrate formation are thermosensitive processes. J Gen Microbiol. 1980 Mar;117(1):243–247. doi: 10.1099/00221287-117-1-243. [DOI] [PubMed] [Google Scholar]
  7. Dutton P. L., Prince R. C. Equilibrium and disequilibrium in the ubiquinone-cytochrome b-c2 oxidoreductase or Rhodopseudomonas sphaeroides. FEBS Lett. 1978 Jul 1;91(1):15–20. doi: 10.1016/0014-5793(78)80007-6. [DOI] [PubMed] [Google Scholar]
  8. Fraley R. T., Lueking D. R., Kaplan S. Intracytoplasmic membrane synthesis in synchronous cell populations of Rhodopseudomonas sphaeroides. Polypeptide insertion into growing membrane. J Biol Chem. 1978 Jan 25;253(2):458–464. [PubMed] [Google Scholar]
  9. Fraley R. T., Lueking D. R., Kaplan S. The relationship of intracytoplasmic membrane assembly to the cell division cycle in Rhodopseudomonas sphaeroides. J Biol Chem. 1979 Mar 25;254(6):1980–1986. [PubMed] [Google Scholar]
  10. Fraley R. T., Yen G. S., Lueking D. R., Kaplan S. The physical state of the intracytoplasmic membrane of Rhodopseudomonas sphaeroides and its relationship to the cell division cycle. J Biol Chem. 1979 Mar 25;254(6):1987–1991. [PubMed] [Google Scholar]
  11. Gast P., Hoff A. J. Determination of the decay rates of the triplet state of Rhodopseudomonas sphaeroides by fast laser-flash ESR spectroscopy. FEBS Lett. 1978 Jan 1;85(1):183–188. doi: 10.1016/0014-5793(78)81276-9. [DOI] [PubMed] [Google Scholar]
  12. Lueking D. R., Fraley R. T., Kaplan S. Intracytoplasmic membrane synthesis in synchronous cell populations of Rhodopseudomonas sphaeroides. Fate of "old" and "new" membrane. J Biol Chem. 1978 Jan 25;253(2):451–457. [PubMed] [Google Scholar]
  13. MUNKRES K. D., RICHARDS F. M. THE PURIFICATION AND PROPERTIES OF NEUROSPORA MALATE DEHYDROGENASE. Arch Biochem Biophys. 1965 Mar;109:466–479. doi: 10.1016/0003-9861(65)90391-7. [DOI] [PubMed] [Google Scholar]
  14. Miller L., Kaplan S. Plasmid transfer and expression in Rhodopseudomonas sphaeroides. Arch Biochem Biophys. 1978 Apr 15;187(1):229–234. doi: 10.1016/0003-9861(78)90028-0. [DOI] [PubMed] [Google Scholar]
  15. Oelze J., Drews G. Membranes of photosynthetic bacteria. Biochim Biophys Acta. 1972 Apr 18;265(2):209–239. doi: 10.1016/0304-4157(72)90003-2. [DOI] [PubMed] [Google Scholar]
  16. Prince R. C., Dutton P. L. Single and multiple turnover reactions in the ubiquinone-cytochrome b-c2 oxidoreductase of Rhodopseudomonas sphaeroids: the physical chemistry of the major electron donor to cytochrome c2, and its coupled reactions. Biochim Biophys Acta. 1977 Dec 23;462(3):731–747. doi: 10.1016/0005-2728(77)90114-1. [DOI] [PubMed] [Google Scholar]
  17. Reed D. W., Clayton R. K. Isolation of a reaction center fraction from Rhodopseudomonas spheroides. Biochem Biophys Res Commun. 1968 Mar 12;30(5):471–475. doi: 10.1016/0006-291x(68)90075-2. [DOI] [PubMed] [Google Scholar]
  18. Reed D. W. Isolation and composition of a photosynthetic reaction center complex from Rhodopseudomonas spheroides. J Biol Chem. 1969 Sep 25;244(18):4936–4941. [PubMed] [Google Scholar]
  19. SISTROM W. R. The kinetics of the synthesis of photopigments in Rhodopseudomonas spheroides. J Gen Microbiol. 1962 Sep;28:607–616. doi: 10.1099/00221287-28-4-607. [DOI] [PubMed] [Google Scholar]
  20. Sauer K., Austin L. A. Bacteriochlorophyll-protein complexes from the light-harvesting antenna of photosynthetic bacteria. Biochemistry. 1978 May 16;17(10):2011–2019. doi: 10.1021/bi00603a033. [DOI] [PubMed] [Google Scholar]
  21. Tabita F. R., Caruso P., Whitman W. Facile assay of enzymes unique to the Calvin cycle in intact cells, with special reference to ribulose 1,5-bisphosphate carboxylase. Anal Biochem. 1978 Feb;84(2):462–472. doi: 10.1016/0003-2697(78)90064-7. [DOI] [PubMed] [Google Scholar]
  22. Takamiya K., Dutton P. L. The influence of transmembrane potentials of the redox equilibrium between cytochrome c2 and the reaction center in Rhodopseudomonas sphaeroides chromatophores. FEBS Lett. 1977 Aug 15;80(2):279–284. doi: 10.1016/0014-5793(77)80457-2. [DOI] [PubMed] [Google Scholar]
  23. WORDEN P. B., SISTROM W. R. THE PREPARATION AND PROPERTIES OF BACTERIAL CHROMATOPHORE FRACTIONS. J Cell Biol. 1964 Oct;23:135–150. doi: 10.1083/jcb.23.1.135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wraight C. A., Lueking D. R., Fraley R. T., Kaplan S. Synthesis of photopigments and electron transport components in synchronous phototrophic cultures of Rhodopseudomonas sphaeroides. J Biol Chem. 1978 Jan 25;253(2):465–471. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES