Abstract
Sporangiophores of the fungus Phycomyces exhibit adaptation to light stimuli over a dynamic range of 10(10). This range applies to both phototropism and the closely related light-growth response; in the latter response, the elongation rate is modulated transiently by changes in the light intensity. We have performed light- and dark- adaptation experiments on growing sporangiophores using an automated tracking machine that allows a continuous measurement of growth velocity under controlled conditions. The results are examined in terms of the adaptation model of Delbruck and Reichardt (1956, Cellular Mechanisms in Differentiation and Growth, 3-44). The "level of adaptation," A, was inferred from responses to test pulses of light by means of a series of intensity-response curves. For dark adaptation to steps down in the normal intensity range (10(-6)-10(-2) W/m2), A decays exponentially with a time constant b = 6.1 +/- 0.3 min. This result is in agreement with the model. Higher-order kinetics are indicated, however, for dark adaptation in the high-intensity range (10(-2)-1 W/m2). Adaptation in this range is compared with predictions of a model relating changes in A to the inactivation and recovery of a receptor pigment. In response to steps up in intensity in the normal range, A was found to increase rapidly, overshoot the applied intensity level, and then relax to that level within 40 min. These results are incompatible with the Delbruck-Reichardt model or any simple generalizations of it. The asymmetry and overshoot are similar to adaptation phenomena observed in systems as diverse as bacterial chemotaxis and human vision. It appears likely that light and dark adaptation in Phycomyces are mediated by altogether different processes.
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- BAKER H. D. Initial stages of dark and light adaptation. J Opt Soc Am. 1963 Jan;53:98–103. doi: 10.1364/josa.53.000098. [DOI] [PubMed] [Google Scholar]
- Berg H. C., Tedesco P. M. Transient response to chemotactic stimuli in Escherichia coli. Proc Natl Acad Sci U S A. 1975 Aug;72(8):3235–3239. doi: 10.1073/pnas.72.8.3235. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bergman K., Burke P. V., Cerdá-Olmedo E., David C. N., Delbrück M., Foster K. W., Goodell E. W., Heisenberg M., Meissner G., Zalokar M. Phycomyces. Bacteriol Rev. 1969 Mar;33(1):99–157. doi: 10.1128/br.33.1.99-157.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dennison D. S., Foster K. W. Intracellular rotation and the phototropic response of Phycomyces. Biophys J. 1977 Apr;18(1):103–123. doi: 10.1016/S0006-3495(77)85599-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Foster K. W., Lipson E. D. The light growth response of Phycomyces. J Gen Physiol. 1973 Nov;62(5):590–617. doi: 10.1085/jgp.62.5.590. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lipson E. D. White noise analysis of Phycomyces light growth response system. I. Normal intensity range. Biophys J. 1975 Oct;15(10):989–1011. doi: 10.1016/S0006-3495(75)85879-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lipson E. D. White noise analysis of Phycomyces light growth response system. II. Extended intensity ranges. Biophys J. 1975 Oct;15(10):1013–1031. doi: 10.1016/S0006-3495(75)85880-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lipson E. D. White noise analysis of Phycomyces light growth response system. III. Photomutants. Biophys J. 1975 Oct;15(10):1033–1045. doi: 10.1016/S0006-3495(75)85881-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Medina J. R., Cerdá-Olmedo E. A quantitative model of Phycomyces phototropism. J Theor Biol. 1977 Dec 21;69(4):709–719. doi: 10.1016/0022-5193(77)90377-0. [DOI] [PubMed] [Google Scholar]
- Otto M. K., Jayaram M., Hamilton R. M., Delbrück M. Replacement of riboflavin by an analogue in the blue-light photoreceptor of Phycomyces. Proc Natl Acad Sci U S A. 1981 Jan;78(1):266–269. doi: 10.1073/pnas.78.1.266. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RUSHTON W. A. VISUAL ADAPTATION. Proc R Soc Lond B Biol Sci. 1965 Mar 16;162:20–46. doi: 10.1098/rspb.1965.0024. [DOI] [PubMed] [Google Scholar]
- Springer M. S., Goy M. F., Adler J. Protein methylation in behavioural control mechanisms and in signal transduction. Nature. 1979 Jul 26;280(5720):279–284. doi: 10.1038/280279a0. [DOI] [PubMed] [Google Scholar]