Abstract
Mutants of Escherichia coli resistant to allyl alcohol were selected. Such mutants were found to lack alcohol dehydrogenase. In addition, mutants with temperature-sensitive alcohol dehydrogenase activity were obtained. These mutations, designated adhE, are all located at the previously described adh regulatory locus. Most adhE mutants were also defective in acetaldehyde dehydrogenase activity.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Alston T. A., Mela L., Bright H. J. Inactivation of alcohol dehydrogenase by 3-butyn-1-ol. Arch Biochem Biophys. 1979 Oct 15;197(2):516–523. doi: 10.1016/0003-9861(79)90275-3. [DOI] [PubMed] [Google Scholar]
- Bachmann B. J., Low K. B. Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980 Mar;44(1):1–56. doi: 10.1128/mr.44.1.1-56.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bochner B. R., Savageau M. A. Generalized indicator plate for genetic, metabolic, and taxonomic studies with microorganisms. Appl Environ Microbiol. 1977 Feb;33(2):434–444. doi: 10.1128/aem.33.2.434-444.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ciriacy M. Genetics of alcohol dehydrogenase in Saccharomyces cerevisiae. II. Two loci controlling synthesis of the glucose-repressible ADH II. Mol Gen Genet. 1975;138(2):157–164. doi: 10.1007/BF02428119. [DOI] [PubMed] [Google Scholar]
- Clark D. P., Cronan J. E., Jr Acetaldehyde coenzyme A dehydrogenase of Escherichia coli. J Bacteriol. 1980 Oct;144(1):179–184. doi: 10.1128/jb.144.1.179-184.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clark D., Cronan J. E., Jr Escherichia coli mutants with altered control of alcohol dehydrogenase and nitrate reductase. J Bacteriol. 1980 Jan;141(1):177–183. doi: 10.1128/jb.141.1.177-183.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DAWES E. A., FOSTER S. M. The formation of ethanol in Escherichia coli. Biochim Biophys Acta. 1956 Nov;22(2):253–265. doi: 10.1016/0006-3002(56)90148-2. [DOI] [PubMed] [Google Scholar]
- Rando R. R. Allyl alcohol-induced irreversible inhibition of yeast alcohol dehydrogenase. Biochem Pharmacol. 1974 Aug 15;23(16):2328–2331. doi: 10.1016/0006-2952(74)90563-2. [DOI] [PubMed] [Google Scholar]
- Rando R. R. In situ generation of irreversible enzyme inhibitors. Nat New Biol. 1972 May 10;237(71):53–53. doi: 10.1038/newbio237053a0. [DOI] [PubMed] [Google Scholar]
- Rudolph F. B., Purich D. L., Fromm H. J. Coenzyme A-linked aldehyde dehydrogenase from Escherichia coli. I. Partial purification, properties, and kinetic studies of the enzyme. J Biol Chem. 1968 Nov 10;243(21):5539–5545. [PubMed] [Google Scholar]
- Schmitt B. Aldehyde dehydrogenase activity of a complex particle from E. coli. Biochimie. 1975;57(9):1001–1004. doi: 10.1016/s0300-9084(75)80355-5. [DOI] [PubMed] [Google Scholar]