Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1982 Nov;152(2):943–945. doi: 10.1128/jb.152.2.943-945.1982

Heme Inhibition of Ferrisiderophore Reductase in Bacillus subtilis

J S Lodge 1,, C G Gaines 1,, J E L Arceneaux 1, B R Byers 1
PMCID: PMC221558  PMID: 6813318

Abstract

Heme was a noncompetitive inhibitor (apparent Ki and K′i = 0.043 mM) of a ferrisiderophore reductase purified from Bacillus subtilis; protoporphyrin IX had no effect. The cellular level of heme may partly regulate the function of this reductase to yield a controlled flow of iron into metabolism.

Full text

PDF
943

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arceneaux J. E., Byers B. R. Ferrisiderophore reductase activity in Bacillus megaterium. J Bacteriol. 1980 Feb;141(2):715–721. doi: 10.1128/jb.141.2.715-721.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BURNHAM B. F., PIERCE W. S., WILLIAMS K. R., BOYER M. H., KIRBY C. K. delta-aminolaevulate dehydratase from Rhodopseudomonas spheroides. Biochem J. 1963 Jun;87:462–472. doi: 10.1042/bj0870462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brown K. A., Ratledge C. Iron transport in Mycobacterium smegmatis: ferrimycobactin reductase (nad(p)h:ferrimycobactin oxidoreductase), the enzyme releasing iron from its carrier. FEBS Lett. 1975 May 1;53(2):262–266. doi: 10.1016/0014-5793(75)80033-0. [DOI] [PubMed] [Google Scholar]
  4. Cooper S. R., McArdle J. V., Raymond K. N. Siderophore electrochemistry: relation to intracellular iron release mechanism. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3551–3554. doi: 10.1073/pnas.75.8.3551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cornish-Bowden A. A simple graphical method for determining the inhibition constants of mixed, uncompetitive and non-competitive inhibitors. Biochem J. 1974 Jan;137(1):143–144. doi: 10.1042/bj1370143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cox C. D. Iron reductases from Pseudomonas aeruginosa. J Bacteriol. 1980 Jan;141(1):199–204. doi: 10.1128/jb.141.1.199-204.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DIXON M. The determination of enzyme inhibitor constants. Biochem J. 1953 Aug;55(1):170–171. doi: 10.1042/bj0550170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ernst J. F., Winkelmann G. Enzymatic release of iron from sideramines in fungi. NADH:sideramine oxidoreductase in Neurospora crassa. Biochim Biophys Acta. 1977 Nov 7;500(1):27–41. doi: 10.1016/0304-4165(77)90043-5. [DOI] [PubMed] [Google Scholar]
  9. Gaines C. G., Lodge J. S., Arceneaux J. E., Byers B. R. Ferrisiderophore reductase activity associated with an aromatic biosynthetic enzyme complex in Bacillus subtilis. J Bacteriol. 1981 Nov;148(2):527–533. doi: 10.1128/jb.148.2.527-533.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Lascelles J., Hatch T. P. Bacteriochlorophyll and heme synthesis in Rhodopseudomonas spheroides: possible role of heme in regulation of the branched biosynthetic pathway. J Bacteriol. 1969 May;98(2):712–720. doi: 10.1128/jb.98.2.712-720.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lodge J. S., Gaines C. G., Arceneaux J. E., Byers B. R. Ferrisiderophore reductase activity in Agrobacterium tumefaciens. J Bacteriol. 1982 Feb;149(2):771–774. doi: 10.1128/jb.149.2.771-774.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lodge J. S., Gaines C. G., Arceneaux J. E., Byers B. R. Non-hydrolytic release of iron from ferrienterobactin analogs by extracts of Bacillus subtilis. Biochem Biophys Res Commun. 1980 Dec 31;97(4):1291–1295. doi: 10.1016/s0006-291x(80)80006-4. [DOI] [PubMed] [Google Scholar]
  14. Straka J. G., Emery T. The role of ferrichrome reductase in iron metabolism of Ustilago sphaerogena. Biochim Biophys Acta. 1979 Aug 15;569(2):277–286. doi: 10.1016/0005-2744(79)90063-9. [DOI] [PubMed] [Google Scholar]
  15. Tait G. H. The identification and biosynthesis of siderochromes formed by Micrococcus denitrificans. Biochem J. 1975 Jan;146(1):191–204. doi: 10.1042/bj1460191. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES