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. 1982 Nov;152(2):959–962. doi: 10.1128/jb.152.2.959-962.1982

Bacteriophage P22 as a vector for Mu mutagenesis in Salmonella typhimurium: isolation of nad-lac and pnc-lac gene fusions.

E A Holley, J W Foster
PMCID: PMC221562  PMID: 6752128

Abstract

Salmonella phage P22 was utilized as a vector for phage Mu cts d1(Apr lac) mutagenesis in Salmonella typhimurium. Efficient transposition of phage Mu d1 and the construction of gene fusions were readily accomplished with this procedure. Mutants blocked in the biosynthesis of NAD+ and in pyridine nucleotide cycle metabolism were isolated by this method, resulting in nadB-lac, nadC-lac, and pncB-lac gene fusions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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