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. 1982 Mar 1;79(3):481–505. doi: 10.1085/jgp.79.3.481

Gallbladder epithelial cell hydraulic water permeability and volume regulation

PMCID: PMC2215760  PMID: 7077291

Abstract

The hydraulic water permeability (Lp) of the cell membranes of Necturus gallbladder epithelial cells was estimated from the rate of change of cell volume after a change in the osmolality of the bathing solution. Cell volume was calculated from computer reconstruction of light microscopic images of epithelial cells obtained by the "optical slice" technique. The tissue was mounted in a miniature Ussing chamber designed to achieve optimal optical properties, rapid bath exchange, and negligible unstirred layer thickness. The control solution contained only 80% of the normal NaCl concentration, the remainder of the osmolality was made up by mannitol, a condition that did not significantly decrease the fluid absorption rate in gallbladder sac preparations. The osmotic gradient ranged from 11.5 to 41 mosmol and was achieved by the addition or removal of mannitol from the perfusion solutions. The Lp of the apical membrane of the cell was 1.0 X 10(-3) cm/s . osmol (Posm = 0.055 cm/s) and that of the basolateral membrane was 2.2 X 10(-3) cm/s . osmol (Posm = 0.12 cm/s). These values were sufficiently high so that normal fluid absorption by Necturus gallbladder could be accomplished by a 2.4-mosmol solute gradient across the apical membrane and a 1.1-mosmol gradient across the basolateral membrane. After the initial cell shrinkage or swelling resulting from the anisotonic mucosal or serosal medium, cell volume returned rapidly toward the control value despite the fact that one bathing solution remained anisotonic. This volume regulatory response was not influenced by serosal ouabain or reduction of bath NaCl concentration to 10 mM. Complete removal of mucosal perfusate NaCl abolished volume regulation after cell shrinkage. Estimates were also made of the reflection coefficient for NaCl and urea at the apical cell membrane and of the velocity of water flow across the cytoplasm.

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Selected References

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  1. Andreoli T. E., Schafer J. A., Troutman S. L. Perfusion rate-dependence of transepithelial osmosis in isolated proximal convoluted tubules: estimation of the hydraulic conductance. Kidney Int. 1978 Sep;14(3):263–269. doi: 10.1038/ki.1978.118. [DOI] [PubMed] [Google Scholar]
  2. Cala P. M. Volume regulation by Amphiuma red blood cells. The membrane potential and its implications regarding the nature of the ion-flux pathways. J Gen Physiol. 1980 Dec;76(6):683–708. doi: 10.1085/jgp.76.6.683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DIAMOND J. M. The reabsorptive function of the gall-bladder. J Physiol. 1962 May;161:442–473. doi: 10.1113/jphysiol.1962.sp006898. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dellasega M., Grantham J. J. Regulation of renal tubule cell volume in hypotonic media. Am J Physiol. 1973 Jun;224(6):1288–1294. doi: 10.1152/ajplegacy.1973.224.6.1288. [DOI] [PubMed] [Google Scholar]
  5. DiBona D. R., Civan M. M., Leaf A. The anatomic site of the transepithelial permeability barriers of toad bladder. J Cell Biol. 1969 Jan;40(1):1–7. doi: 10.1083/jcb.40.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DiBona D. R., Civan M. M. Pathways for movement of ions and water across toad urinary bladder. I. Anatomic site of transepithelial shunt pathways. J Membr Biol. 1973;12(2):101–128. doi: 10.1007/BF01869994. [DOI] [PubMed] [Google Scholar]
  7. Diamond J. M. Osmotic water flow in leaky epithelia. J Membr Biol. 1979 Dec 31;51(3-4):195–216. doi: 10.1007/BF01869084. [DOI] [PubMed] [Google Scholar]
  8. Fettiplace R., Haydon D. A. Water permeability of lipid membranes. Physiol Rev. 1980 Apr;60(2):510–550. doi: 10.1152/physrev.1980.60.2.510. [DOI] [PubMed] [Google Scholar]
  9. Fischbarg J., Warshavsky C. R., Lim J. J. Pathways for hydraulically and osmotically-induced water flows across epithelia. Nature. 1977 Mar 3;266(5597):71–74. doi: 10.1038/266071a0. [DOI] [PubMed] [Google Scholar]
  10. Ginn F. L., Shelburne J. D., Trump B. F. Disorders of cell volume regulation. I. Effects of inhibition of plasma membrane adenosine triphosphatase with ouabain. Am J Pathol. 1968 Dec;53(6):1041–1071. [PMC free article] [PubMed] [Google Scholar]
  11. Grantham J. J., Lowe C. M., Dellasega M., Cole B. R. Effect of hypotonic medium on K and Na content of proximal renal tubules. Am J Physiol. 1977 Jan;232(1):F42–F49. doi: 10.1152/ajprenal.1977.232.1.F42. [DOI] [PubMed] [Google Scholar]
  12. HAYS R. M., LEAF A. Studies on the movement of water through the isolated toad bladder and its modification by vasopressin. J Gen Physiol. 1962 May;45:905–919. doi: 10.1085/jgp.45.5.905. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. HOUSE C. R. THE NATURE OF WATER TRANSPORT ACROSS FROG SKIN. Biophys J. 1964 Sep;4:401–416. doi: 10.1016/s0006-3495(64)86791-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hill A. Salt-water coupling in leaky epithelia. J Membr Biol. 1980 Oct 31;56(3):177–182. doi: 10.1007/BF01869474. [DOI] [PubMed] [Google Scholar]
  15. Johnson J. A., Wilson T. A. Osmotic volume changes induced by a permeable solute. J Theor Biol. 1967 Nov;17(2):304–311. doi: 10.1016/0022-5193(67)90173-7. [DOI] [PubMed] [Google Scholar]
  16. Kregenow F. M. Functional separation of the Na-K exchange pump from the volume controlling mechanism in enlarged duck red cells. J Gen Physiol. 1974 Oct;64(4):393–412. doi: 10.1085/jgp.64.4.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. LINDEMANN B., SOLOMON A. K. Permeability of luminal surface of intestinal mucosal cells. J Gen Physiol. 1962 Mar;45:801–810. doi: 10.1085/jgp.45.4.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. MACROBBIE E. A., USSING H. H. Osmotic behaviour of the epithelial cells of frog skin. Acta Physiol Scand. 1961 Nov-Dec;53:348–365. doi: 10.1111/j.1748-1716.1961.tb02293.x. [DOI] [PubMed] [Google Scholar]
  19. Pedley T. J., Fischbarg J. Unstirred layer effects in osmotic water flow across gallbladder epithelium. J Membr Biol. 1980 May 23;54(2):89–102. doi: 10.1007/BF01940563. [DOI] [PubMed] [Google Scholar]
  20. Rich G. T., Sha'afi I., Romualdez A., Solomon A. K. Effect of osmolality on the hydraulic permeability coefficient of red cells. J Gen Physiol. 1968 Dec;52(6):941–954. doi: 10.1085/jgp.52.6.941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sackin H., Boulpaep E. L. Models for coupling of salt and water transport; Proximal tubular reabsorption in Necturus kidney. J Gen Physiol. 1975 Dec;66(6):671–733. doi: 10.1085/jgp.66.6.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schafer J. A., Patlak C. S., Troutman S. L., Andreoli T. E. Volume absorption in the pars recta. II. Hydraulic conductivity coefficient. Am J Physiol. 1978 Apr;234(4):F340–F348. doi: 10.1152/ajprenal.1978.234.4.F340. [DOI] [PubMed] [Google Scholar]
  23. Smyth D. H., Wright E. M. Streaming potentials in the rat small intestine. J Physiol. 1966 Feb;182(3):591–602. doi: 10.1113/jphysiol.1966.sp007839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Spring K. R., Hope A. Fluid transport and the dimensions of cells and interspaces of living Necturus gallbladder. J Gen Physiol. 1979 Mar;73(3):287–305. doi: 10.1085/jgp.73.3.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Spring K. R., Hope A. Size and shape of the lateral intercellular spaces in a living epithelium. Science. 1978 Apr 7;200(4337):54–58. doi: 10.1126/science.635571. [DOI] [PubMed] [Google Scholar]
  26. Terwilliger T. C., Solomon A. K. Osmotic water permeability of human red cells. J Gen Physiol. 1981 May;77(5):549–570. doi: 10.1085/jgp.77.5.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. WHITTEMBURY G. Action of antidiuretic hormone on the equivalent pore radius at both surfaces of the epithelium of the isolated toad skin. J Gen Physiol. 1962 Sep;46:117–130. doi: 10.1085/jgp.46.1.117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. van Os C. H., Wiedner G., Wright E. M. Volume flows across gallbladder epithelium induced by small hydrostatic and osmotic gradients. J Membr Biol. 1979 Aug;49(1):1–20. doi: 10.1007/BF01871037. [DOI] [PubMed] [Google Scholar]

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