Skip to main content
NCBI home page
The Journal of General Physiology logoLink to The Journal of General Physiology
. 1986 Apr 1;87(4):551–566. doi: 10.1085/jgp.87.4.551

Catecholamine-induced transport systems in trout erythrocyte. Na+/H+ countertransport or NaCl cotransport?

PMCID: PMC2215878  PMID: 3701298

Abstract

It has previously been shown (Baroin, A., F. Garcia-Romeu, T. Lamarre, and R. Motais. 1984a, b. Journal of Physiology. 350:137, 356:21; Mahe, Y., F. Garcia-Romeu, and R. Motais. 1985. European Journal of Pharmacology. 116:199) that the addition of catecholamines to an isotonic suspension of nucleated red blood cells of the rainbow trout first stimulates a cAMP-dependent, amiloride-sensitive Na+/H+ exchange. This stimulation seems to be transient. It is followed by a more permanent activation of a coupled entry of Na+ and Cl-, which is inhibited by amiloride but also by inhibitors of band 3 protein (DIDS, furosemide, niflumic acid). The coupled entry of Na+ and Cl- could therefore result from the parallel and simultaneous exchange of Na+out for H+in (via the cAMP-dependent Na+/H+ antiporter) and Cl- out for HCO3- in (via the anion exchange system located in band 3 protein). However, in view of the following arguments, it had been proposed that NaCl uptake does not proceed by the double-exchanger system but via an NaCl cotransport: (a) Na+ entry requires Cl- as anion (in NO3- medium, the Na uptake is strongly inhibited, whereas NO3- is an extremely effective substitute for Cl- in the anion exchange system); (b) Na uptake is not significantly affected by the presence of HCO3- in the suspension medium despite the fact that in red cells, Cl-/HCO3- exchange occurs more readily than the exchanges of Cl- for basic equivalents in a theoretically CO2-free medium (the so-called Cl-/OH- exchanges). The purpose of the present paper was a reassessment of the two models by using monensin, an ionophore allowing Na+/H+ exchange. From this study, it appears that NaCl entry results from the simultaneous functioning of the Na+/H+ antiporter and the anion exchange system. The apparent Cl dependence is explained by the fact that, in these erythrocytes, NO3- clearly inhibits the turnover rate of the Na+/H+ antiporter. As Na+/H+ exchange is the driving component in the salt uptake process, this inhibition explains the Cl requirement for Na entry. The lack of stimulation of cell swelling by bicarbonate is explained by the fact that the rate of anion exchange in a CO2-free medium (Cl-/OH- exchange) is roughly equivalent to that of Na+/H+ exchange and thus in practice is not limiting to the net influx of NaCl through the two exchangers.(ABSTRACT TRUNCATED AT 400 WORDS)

Full Text

The Full Text of this article is available as a PDF (961.8 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aubert L., Motais R. Molecular features of organic anion permeablity in ox red blood cell. J Physiol. 1975 Mar;246(1):159–179. doi: 10.1113/jphysiol.1975.sp010884. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baroin A., Garcia-Romeu F., Lamarre T., Motais R. A transient sodium-hydrogen exchange system induced by catecholamines in erythrocytes of rainbow trout, Salmo gairdneri. J Physiol. 1984 Nov;356:21–31. doi: 10.1113/jphysiol.1984.sp015450. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baroin A., Garcia-Romeu F., Lamarre T., Motais R. Hormone-induced co-transport with specific pharmacological properties in erythrocytes of rainbow trout, Salmo gairdneri. J Physiol. 1984 May;350:137–157. doi: 10.1113/jphysiol.1984.sp015193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brazy P. C., Gunn R. B. Furosemide inhibition of chloride transport in human red blood cells. J Gen Physiol. 1976 Dec;68(6):583–599. doi: 10.1085/jgp.68.6.583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cousin J. L., Motais R., Sola F. Transmembrane exchange of chloride with bicarbonate ion in mammalian red blood cells: evidence for a sulphonamide-sensitive "carrier". J Physiol. 1975 Dec;253(2):385–399. doi: 10.1113/jphysiol.1975.sp011195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cousin J. L., Motais R. The role of carbonic anhydrase inhibitors on anion permeability into ox red blood cells. J Physiol. 1976 Mar;256(1):61–80. doi: 10.1113/jphysiol.1976.sp011311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Crandall E. D., Klocke R. A., Forster R. E. Hydroxyl ion movements across the human erythrocyte membrane. Measurement of rapid pH changes in red cell suspensions. J Gen Physiol. 1971 Jun;57(6):664–683. doi: 10.1085/jgp.57.6.664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ellory J. C., Dunham P. B., Logue P. J., Stewart G. W. Anion-dependent cation transport in erythrocytes. Philos Trans R Soc Lond B Biol Sci. 1982 Dec 1;299(1097):483–495. doi: 10.1098/rstb.1982.0146. [DOI] [PubMed] [Google Scholar]
  9. Jennings M. L. Proton fluxes associated with erythrocyte membrane anion exchange. J Membr Biol. 1976 Aug 26;28(2-3):187–205. doi: 10.1007/BF01869697. [DOI] [PubMed] [Google Scholar]
  10. Mahé Y., Garcia-Romeu F., Motais R. Inhibition by amiloride of both adenylate cyclase activity and the Na+/H+ antiporter in fish erythrocytes. Eur J Pharmacol. 1985 Oct 22;116(3):199–206. doi: 10.1016/0014-2999(85)90154-2. [DOI] [PubMed] [Google Scholar]
  11. Motais R., Cousin J. L., Sola F. The chloride transport induced by triaklyl-tin compound across erythrocyte membrane. Biochim Biophys Acta. 1977 Jun 16;467(3):357–363. doi: 10.1016/0005-2736(77)90313-3. [DOI] [PubMed] [Google Scholar]
  12. Parker J. C. Glutaraldehyde fixation of sodium transport in dog red blood cells. J Gen Physiol. 1984 Nov;84(5):789–803. doi: 10.1085/jgp.84.5.789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Parker J. C. Volume-responsive sodium movements in dog red blood cells. Am J Physiol. 1983 May;244(5):C324–C330. doi: 10.1152/ajpcell.1983.244.5.C324. [DOI] [PubMed] [Google Scholar]
  14. Selwyn M. J., Dawson A. P., Stockdale M., Gains N. Chloride-hydroxide exchange across mitochondrial, erythrocyte and artificial lipid membranes mediated by trialkyl- and triphenyltin compounds. Eur J Biochem. 1970 May 1;14(1):120–126. doi: 10.1111/j.1432-1033.1970.tb00268.x. [DOI] [PubMed] [Google Scholar]
  15. Tosteson M. T., Wieth J. O. Tributyltin-mediated exchange diffusion of halides in lipid bilayers. J Gen Physiol. 1979 Jun;73(6):789–800. doi: 10.1085/jgp.73.6.789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wieth J. O., Tosteson M. T. Organotin-mediated exchange diffusion of anions in human red cells. J Gen Physiol. 1979 Jun;73(6):765–788. doi: 10.1085/jgp.73.6.765. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of General Physiology are provided here courtesy of The Rockefeller University Press

RESOURCES