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. 1987 Mar 1;89(3):405–420. doi: 10.1085/jgp.89.3.405

Mitogen induction of ion channels in murine T lymphocytes

PMCID: PMC2215901  PMID: 2435845

Abstract

Using gigohm-seal recording, we studied ion channel expression in resting and activated T lymphocytes from mice. Both the number of channels per cell and the predominant type of K+ channel depend upon the state of activation of the cell. Unstimulated T cells express small numbers of K+ channels, typically a dozen per cell, and are heterogeneous, usually expressing either type n or type l K+ channels (see DeCoursey, T. E., K. G. Chandy, S. Gupta, and M. D. Cahalan. 1987. Journal of General Physiology. 89:379-404). 1 d after stimulation by the murine T cell mitogen concanavalin A, large numbers of type n K+ channels appear in enlarged, activated cells. Type n channels appear in activated cells with a time course consistent with that reported for mitogen-induced enhancement of protein synthesis. Voltage-gated tetrodotoxin-sensitive Na+ channels present in about one-third of unstimulated cells from the MRL-n strain are increased approximately 10- fold after activation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Averdunk R. Uber die Wirkung von Phytohänagglutinin und Antilymphozytenserum auf den Kalium-, Glucose- und Aminosäure-Transport bei menschlichen Lymphozyten. Hoppe Seylers Z Physiol Chem. 1972 Jan;353(1):79–87. doi: 10.1515/bchm2.1972.353.1.79. [DOI] [PubMed] [Google Scholar]
  2. Biberfeld P. Morphogenesis in blood lymphocytes stimulated with phytohaemagglutinin (PHA). A light and electron microscopic study. Acta Pathol Microbiol Scand Suppl. 1971;223(Suppl):1–70. [PubMed] [Google Scholar]
  3. Chandy K. G., DeCoursey T. E., Cahalan M. D., Gupta S. Electroimmunology: the physiologic role of ion channels in the immune system. J Immunol. 1985 Aug;135(2 Suppl):787s–791s. [PubMed] [Google Scholar]
  4. Chandy K. G., DeCoursey T. E., Fischbach M., Talal N., Cahalan M. D., Gupta S. Altered K+ channel expression in abnormal T lymphocytes from mice with the lpr gene mutation. Science. 1986 Sep 12;233(4769):1197–1200. doi: 10.1126/science.2426784. [DOI] [PubMed] [Google Scholar]
  5. DeCoursey T. E., Chandy K. G., Gupta S., Cahalan M. D. Voltage-dependent ion channels in T-lymphocytes. J Neuroimmunol. 1985 Nov;10(1):71–95. doi: 10.1016/0165-5728(85)90035-9. [DOI] [PubMed] [Google Scholar]
  6. DeCoursey T. E., Chandy K. G., Gupta S., Cahalan M. D. Voltage-gated K+ channels in human T lymphocytes: a role in mitogenesis? Nature. 1984 Feb 2;307(5950):465–468. doi: 10.1038/307465a0. [DOI] [PubMed] [Google Scholar]
  7. Decoursey T. E., Chandy K. G., Gupta S., Cahalan M. D. Two types of potassium channels in murine T lymphocytes. J Gen Physiol. 1987 Mar;89(3):379–404. doi: 10.1085/jgp.89.3.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Deutsch C., Krause D., Lee S. C. Voltage-gated potassium conductance in human T lymphocytes stimulated with phorbol ester. J Physiol. 1986 Mar;372:405–423. doi: 10.1113/jphysiol.1986.sp016016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fukushima Y., Hagiwara S., Henkart M. Potassium current in clonal cytotoxic T lymphocytes from the mouse. J Physiol. 1984 Jun;351:645–656. doi: 10.1113/jphysiol.1984.sp015268. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gallin E. K., Sheehy P. A. Differential expression of inward and outward potassium currents in the macrophage-like cell line J774.1. J Physiol. 1985 Dec;369:475–499. doi: 10.1113/jphysiol.1985.sp015911. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HODGKIN A. L., HUXLEY A. F. A quantitative description of membrane current and its application to conduction and excitation in nerve. J Physiol. 1952 Aug;117(4):500–544. doi: 10.1113/jphysiol.1952.sp004764. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kay J. E. Early effects of phytohaemagglutinin on lymphocyte RNA synthesis. Eur J Biochem. 1968 Apr 3;4(2):225–232. doi: 10.1111/j.1432-1033.1968.tb00198.x. [DOI] [PubMed] [Google Scholar]
  13. Lee S. C., Sabath D. E., Deutsch C., Prystowsky M. B. Increased voltage-gated potassium conductance during interleukin 2-stimulated proliferation of a mouse helper T lymphocyte clone. J Cell Biol. 1986 Apr;102(4):1200–1208. doi: 10.1083/jcb.102.4.1200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lester E. P., Lemkin P., Lipkin L., Cooper H. L. A two-dimensional electrophoretic analysis of protein synthesis in resting and growing lymphocytes in vitro. J Immunol. 1981 Apr;126(4):1428–1434. [PubMed] [Google Scholar]
  15. Matteson D. R., Deutsch C. K channels in T lymphocytes: a patch clamp study using monoclonal antibody adhesion. Nature. 1984 Feb 2;307(5950):468–471. doi: 10.1038/307468a0. [DOI] [PubMed] [Google Scholar]
  16. Owens T., Kaplan J. G. Increased cationic fluxes in stimulated lymphocytes of the mouse: response of enriched B- and T-cell subpopulations to B- and T-cell mitogens. Can J Biochem. 1980 Oct;58(10):831–839. doi: 10.1139/o80-116. [DOI] [PubMed] [Google Scholar]
  17. Quastel M. R., Kaplan J. G. Early stimulation of potassium uptake in lymphocytes treated with PHA. Exp Cell Res. 1970 Nov;63(1):230–233. doi: 10.1016/0014-4827(70)90360-5. [DOI] [PubMed] [Google Scholar]
  18. Rothenberg E., Lugo J. P. Differentiation and cell division in the mammalian thymus. Dev Biol. 1985 Nov;112(1):1–17. doi: 10.1016/0012-1606(85)90114-9. [DOI] [PubMed] [Google Scholar]
  19. Segel G. B., Lichtman M. A. Potasssium transport in human blood lymphocytes treated with phytohemagglutinin. J Clin Invest. 1976 Dec;58(6):1358–1369. doi: 10.1172/JCI108591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sigworth F. J., Neher E. Single Na+ channel currents observed in cultured rat muscle cells. Nature. 1980 Oct 2;287(5781):447–449. doi: 10.1038/287447a0. [DOI] [PubMed] [Google Scholar]
  21. Ypey D. L., Clapham D. E. Development of a delayed outward-rectifying K+ conductance in cultured mouse peritoneal macrophages. Proc Natl Acad Sci U S A. 1984 May;81(10):3083–3087. doi: 10.1073/pnas.81.10.3083. [DOI] [PMC free article] [PubMed] [Google Scholar]

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