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. 1982 Dec;152(3):1175–1187. doi: 10.1128/jb.152.3.1175-1187.1982

Pyruvate-dependent diauxic growth of Rhodospirillum rubrum in light.

D Solaiman, R L Uffen
PMCID: PMC221625  PMID: 6815163

Abstract

When Rhodospirillum rubrum mutant C was first exposed to radiant energy after long-term anaerobic dark growth, the cells often exhibited a diauxic growth response. This happened with pyruvate in the medium and when cultures were exposed to a less-than-growth-saturating white light intensity of about 6,460 lx. Under the growth-saturating light condition, mutant C photometabolized and growth was not affected by Na hypophosphite, an inhibitor of pyruvate fermentation. In lower intensity light, in which diauxie occurred, initial (phase I) growth occurred by fermentation of Na pyruvate and was sensitive to Na hypophosphite inhibition. Once pyruvate was depleted, phase I growth stopped, the bacteriochlorophyll content of the cells began to increase from about 3 nmol/mg of protein, and growth finally resumed phototrophically (phase II). The lag period and phase II growth were influenced by radiant energy. By changing the white light intensity from 2,150 to 753 lx between experiments, the duration of both the lag period and the generation time of cells in phase II growth increased. Diauxic growth was pyruvate dependent. It occurred with pyruvate even if malate, a photometabolizable substrate, was added to the growth medium. Moreover, the biphasic growth response was reversible. It was observed not only with R. rubrum mutant C grown cells photosynthetically, but also when other strains of R. rubrum were placed in pyruvate medium under lowered light conditions. Only R. rubrum S1 did not exhibit the typical pyruvate-dependent diauxic growth response.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aagaard J., Sistrom W. R. Control of synthesis of reaction center bacteriochlorophyll in photosynthetic bacteria. Photochem Photobiol. 1972 Feb;15(2):209–225. doi: 10.1111/j.1751-1097.1972.tb06240.x. [DOI] [PubMed] [Google Scholar]
  2. Atkinson D. E. Regulation of enzyme function. Annu Rev Microbiol. 1969;23:47–68. doi: 10.1146/annurev.mi.23.100169.000403. [DOI] [PubMed] [Google Scholar]
  3. Biedermann M., Drews G., Marx R., Schröder J. Der Einfluss des Sauerstoffpartialdruckes und der Antibiotica Actinomycin und Puromycin auf das Wachstum, die Synthese von Bacteriochlorophyll und die Thylakoidmorphogenese in Dunkelkulturen von Rhodospirillum rubrum. Arch Mikrobiol. 1967 Feb 20;56(2):133–147. [PubMed] [Google Scholar]
  4. Brown A. E., Eiserling F. A., Lascelles J. Bacteriochlorophyll Synthesis and the Ultrastructure of Wild Type and Mutant Strains of Rhodopseudomonas spheroides. Plant Physiol. 1972 Dec;50(6):743–746. doi: 10.1104/pp.50.6.743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CLAYTON R. K. TOWARD THE ISOLATION OF A PHOTOCHEMICAL REACTION CENTER IN RHODOPSEUDOMONAS SPHEROIDES. Biochim Biophys Acta. 1963 Nov 29;75:312–323. doi: 10.1016/0006-3002(63)90618-8. [DOI] [PubMed] [Google Scholar]
  6. COHEN-BAZIRE G., SISTROM W. R., STANIER R. Y. Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Physiol. 1957 Feb;49(1):25–68. doi: 10.1002/jcp.1030490104. [DOI] [PubMed] [Google Scholar]
  7. Chapman A. G., Atkinson D. E. Adenine nucleotide concentrations and turnover rates. Their correlation with biological activity in bacteria and yeast. Adv Microb Physiol. 1977;15:253–306. doi: 10.1016/s0065-2911(08)60318-5. [DOI] [PubMed] [Google Scholar]
  8. Good N. E., Izawa S. Hydrogen ion buffers. Methods Enzymol. 1972;24:53–68. doi: 10.1016/0076-6879(72)24054-x. [DOI] [PubMed] [Google Scholar]
  9. Gorrell T. E., Uffen R. L. Fermentative metabolism of pyruvate by Rhodospirillum rubrum after anaerobic growth in darkness. J Bacteriol. 1977 Aug;131(2):533–543. doi: 10.1128/jb.131.2.533-543.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gorrell T. E., Uffen R. L. Reduction of nicotinamide adenine dinucleotide by pyruvate:lipoate oxidoreductase in anaerobic, dark-grown Rhodospirillum rubrum mutant C. J Bacteriol. 1978 Jun;134(3):830–836. doi: 10.1128/jb.134.3.830-836.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HOLT S. C., MARR A. G. EFFECT OF LIGHT INTENSITY ON THE FORMATION OF INTRACYTOPLASMIC MEMBRANE IN RHODOSPIRILLUM RUBRUM. J Bacteriol. 1965 May;89:1421–1429. doi: 10.1128/jb.89.5.1421-1429.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HUNGATE R. E. The anaerobic mesophilic cellulolytic bacteria. Bacteriol Rev. 1950 Mar;14(1):1–49. doi: 10.1128/br.14.1.1-49.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Irschik H., Oelze J. Membrane differentiation in phototrophically growing Rhodospirillum rubrum during transition from low to high light intensity. Biochim Biophys Acta. 1973 Nov 30;330(1):80–89. doi: 10.1016/0005-2736(73)90286-1. [DOI] [PubMed] [Google Scholar]
  14. KOHLMILLER E. F., Jr, GEST H. A comparative study of the light and dark fermentations of organic acids by Rhodo-spirillum rubrum. J Bacteriol. 1951 Mar;61(3):269–282. doi: 10.1128/jb.61.3.269-282.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LOVENBERG W., BUCHANAN B. B., RABINOWITZ J. C. STUDIES ON THE CHEMICAL NATURE OF CLOSTRIDIAL FERREDOXIN. J Biol Chem. 1963 Dec;238:3899–3913. [PubMed] [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  17. Lien S., Gest H., San Pietro A. Regulation of chlorophyll synthesis in photosynthetic bacteria. J Bioenerg. 1973;4(4):423–434. doi: 10.1007/BF01648969. [DOI] [PubMed] [Google Scholar]
  18. Lien S., San Pietro A., Gest H. Mutational and physiological enhancement of photosynthetic energy conversion in Rhodopseudomonas capsulata. Proc Natl Acad Sci U S A. 1971 Aug;68(8):1912–1915. doi: 10.1073/pnas.68.8.1912. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lüderitz R., Klemme J. H. Isolierung und Charakterisierung eines membrangebundenen Pyruvatdehydrogenase-Komplexes aus dem phototrophen Bakterium Rhodospirillum rubrum. Z Naturforsch C. 1977 May-Jun;32(5-6):351–361. [PubMed] [Google Scholar]
  20. Madigan M. T., Cox J. C., Gest H. Physiology of dark fermentative growth of Rhodopseudomonas capsulata. J Bacteriol. 1980 Jun;142(3):908–915. doi: 10.1128/jb.142.3.908-915.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Madigan M. T., Gest H. Growth of a photosynthetic bacterium anaerobically in darkness, supported by "oxidant-dependent" sugar fermentation. Arch Microbiol. 1978 May 30;117(2):119–122. doi: 10.1007/BF00402298. [DOI] [PubMed] [Google Scholar]
  22. Niederman R. A., Mallon D. E., Parks L. C. Membranes of Rhodopseudomonas sphaeroides. VI. Isolation of a fraction enriched in newly synthesized bacteriochlorophyll alpha-protein complexes. Biochim Biophys Acta. 1979 Aug 7;555(2):210–220. doi: 10.1016/0005-2736(79)90161-5. [DOI] [PubMed] [Google Scholar]
  23. ORMEROD J. G., ORMEROD K. S., GEST H. Light-dependent utilization of organic compounds and photoproduction of molecular hydrogen by photosynthetic bacteria; relationships with nitrogen metabolism. Arch Biochem Biophys. 1961 Sep;94:449–463. doi: 10.1016/0003-9861(61)90073-x. [DOI] [PubMed] [Google Scholar]
  24. Oelze J. Composition and development of the bacterial photosynthetic apparatus. Subcell Biochem. 1981;8:1–73. doi: 10.1007/978-1-4615-7951-9_1. [DOI] [PubMed] [Google Scholar]
  25. Oelze J., Drews G. Membranes of photosynthetic bacteria. Biochim Biophys Acta. 1972 Apr 18;265(2):209–239. doi: 10.1016/0304-4157(72)90003-2. [DOI] [PubMed] [Google Scholar]
  26. Oelze J. Early formation of intracytoplasmic membranes in Rhodospillum rubrum. Biochim Biophys Acta. 1976 Jun 4;436(1):95–100. doi: 10.1016/0005-2736(76)90222-4. [DOI] [PubMed] [Google Scholar]
  27. Oelze J., Schroeder J., Drews G. Bacteriochlorophyll, fatty-acid, and protein synthesis in relation to thylakoid formation in mutant strains of Rhodospirillum rubrum. J Bacteriol. 1970 Mar;101(3):669–674. doi: 10.1128/jb.101.3.669-674.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Schultz J. E., Weaver P. F. Fermentation and anaerobic respiration by Rhodospirillum rubrum and Rhodopseudomonas capsulata. J Bacteriol. 1982 Jan;149(1):181–190. doi: 10.1128/jb.149.1.181-190.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Silverman P. M., Epstein P. M. Cyclic nucleotide metabolism coupled to cytodifferentiation of Blastocladiella emersonii. Proc Natl Acad Sci U S A. 1975 Feb;72(2):442–446. doi: 10.1073/pnas.72.2.442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Swoager W. C., Lindstrom E. S. Isolation and counting of Athiorhodaceae with membrane filters. Appl Microbiol. 1971 Oct;22(4):683–687. doi: 10.1128/am.22.4.683-687.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Uffen R. L. Anaerobic growth of a Rhodopseudomonas species in the dark with carbon monoxide as sole carbon and energy substrate. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3298–3302. doi: 10.1073/pnas.73.9.3298. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Uffen R. L. Effect of low-intensity light on growth response and bacteriochlorophyll concentration in Rhodospirillum rubrum mutant C. J Bacteriol. 1973 Nov;116(2):1086–1088. doi: 10.1128/jb.116.2.1086-1088.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Uffen R. L. Growth properties of Rhodospirillum rubrum mutants and fermentation of pyruvate in anaerobic, dart conditions. J Bacteriol. 1973 Nov;116(2):874–884. doi: 10.1128/jb.116.2.874-884.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Uffen R. L., Sybesma C., Wolfe R. S. Mutants of Rhodospirrillum rubrum obtained after long-term anaerobic, dark growth. J Bacteriol. 1971 Dec;108(3):1348–1356. doi: 10.1128/jb.108.3.1348-1356.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Yen H. C., Marrs B. Growth of Rhodopseudomonas capsulata under anaerobic dark conditions with dimethyl sulfoxide. Arch Biochem Biophys. 1977 Jun;181(2):411–418. doi: 10.1016/0003-9861(77)90246-6. [DOI] [PubMed] [Google Scholar]
  36. Zilinsky J. W., Sojka G. A., Gest H. Energy charge regulation in photosynthetic bacteria. Biochem Biophys Res Commun. 1971 Mar 5;42(5):955–961. doi: 10.1016/0006-291x(71)90523-7. [DOI] [PubMed] [Google Scholar]
  37. del Valle-Tascón S., Ramírez J. M. Origin of the ATP formed during the light-dependent oxygen uptake catalyzed by Rhodospirillum rubrum chromatophores. Z Naturforsch C. 1975 Jan-Feb;30(1):46–52. doi: 10.1515/znc-1975-1-210. [DOI] [PubMed] [Google Scholar]

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