Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1982 Dec;152(3):1280–1283. doi: 10.1128/jb.152.3.1280-1283.1982

Physical mapping of TOL plasmids pWWO and pND2 and various R plasmid-TOL derivatives from Pseudomonas spp.

P R Lehrbach, J Ward, P Meulien, P Broda
PMCID: PMC221643  PMID: 6292166

Abstract

Analysis of several independently isolated R plasmid-TOL hybrids revealed a wide variation in the amount of TOL DNA they contain. If the formation of the various R plasmid-TOL hybrids involves transposition (which has yet to be rigorously assessed), such transposition does not involve a unique segment of TOL DNA.

Full text

PDF
1280

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bayley S. A., Duggleby C. J., Worsey M. J., Williams P. A., Hardy K. G., Broda P. Two modes of loss of the Tol function from Pseudomonas putida mt-2. Mol Gen Genet. 1977 Jul 20;154(2):203–204. doi: 10.1007/BF00330838. [DOI] [PubMed] [Google Scholar]
  2. Chakrabarty A. M., Friello D. A., Bopp L. H. Transposition of plasmid DNA segments specifying hydrocarbon degradation and their expression in various microorganisms. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3109–3112. doi: 10.1073/pnas.75.7.3109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Downing R. G., Duggleby C. J., Villems R., Broda P. An endonuclease cleavage map of the plasmid pWWO-8, a derivative of the TOL plasmid of Pseudomonas putida mt-2. Mol Gen Genet. 1979 Jan 5;168(1):97–99. doi: 10.1007/BF00267938. [DOI] [PubMed] [Google Scholar]
  4. Downing R., Broda P. A cleavage map of the TOL plasmid of Pseudomonas putida mt-2. Mol Gen Genet. 1979;177(1):189–191. doi: 10.1007/BF00267270. [DOI] [PubMed] [Google Scholar]
  5. Duggleby C. J., Bayley S. A., Worsey M. J., Williams P. A., Broda P. Molecular sizes and relationships of TOL plasmids in Pseudomonas. J Bacteriol. 1977 Jun;130(3):1274–1280. doi: 10.1128/jb.130.3.1274-1280.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Franklin F. C., Bagdasarian M., Bagdasarian M. M., Timmis K. N. Molecular and functional analysis of the TOL plasmid pWWO from Pseudomonas putida and cloning of genes for the entire regulated aromatic ring meta cleavage pathway. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7458–7462. doi: 10.1073/pnas.78.12.7458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Franklin F. C., Williams P. A. Construction of a partial diploid for the degradative pathway encoded by the TOL plasmid (pWWO) from Pseudomonas putida mt-2: evidence for the positive nature of the regulation by the xyIR gene. Mol Gen Genet. 1980 Jan;177(2):321–328. doi: 10.1007/BF00267445. [DOI] [PubMed] [Google Scholar]
  8. Gorai A. P., Heffron F., Falkow S., Hedges R. W., Datta N. Electron microscope heteroduplex studies of sequence relationships among plasmids of the W incompatibility group. Plasmid. 1979 Jul;2(3):485–492. doi: 10.1016/0147-619x(79)90032-5. [DOI] [PubMed] [Google Scholar]
  9. Jacoby G. A., Rogers J. E., Jacob A. E., Hedges R. W. Transposition of Pseudomonas toluene-degrading genes and expression in Escherichia coli. Nature. 1978 Jul 13;274(5667):179–180. doi: 10.1038/274179a0. [DOI] [PubMed] [Google Scholar]
  10. Kunz D. A., Chapman P. J. Catabolism of pseudocumene and 3-ethyltoluene by Pseudomonas putida (arvilla) mt-2: evidence for new functions of the TOL (pWWO) plasmid. J Bacteriol. 1981 Apr;146(1):179–191. doi: 10.1128/jb.146.1.179-191.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Meulien P., Downing R. G., Broda P. Excision of the 40kb segment of the TOL plasmid from Pseudomonas putida mt-2 involves direct repeats. Mol Gen Genet. 1981;184(1):97–101. doi: 10.1007/BF00271202. [DOI] [PubMed] [Google Scholar]
  12. Nakazawa T., Hayashi E., Yokota T., Ebina Y., Nakazawa A. Isolation of TOL and RP4 recombinants by integrative suppression. J Bacteriol. 1978 Apr;134(1):270–277. doi: 10.1128/jb.134.1.270-277.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nakazawa T., Inouye S., Nakazawa A. Physical and functional mapping of RP4-TOL plasmid recombinants: analysis of insertion and deletion mutants. J Bacteriol. 1980 Oct;144(1):222–231. doi: 10.1128/jb.144.1.222-231.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ward J. M., Grinsted J. Physical and genetic analysis of the Inc-W group plasmids R388, Sa, and R7K. Plasmid. 1982 May;7(3):239–250. doi: 10.1016/0147-619x(82)90005-1. [DOI] [PubMed] [Google Scholar]
  15. White G. P., Dunn N. W. Apparent fusion of the TOL plasmid with the R91 drug resistance plasmid in Pseudomonas aeruginosa. Aust J Biol Sci. 1977 Aug;30(4):345–355. doi: 10.1071/bi9770345. [DOI] [PubMed] [Google Scholar]
  16. Williams P. A., Murray K. Metabolism of benzoate and the methylbenzoates by Pseudomonas putida (arvilla) mt-2: evidence for the existence of a TOL plasmid. J Bacteriol. 1974 Oct;120(1):416–423. doi: 10.1128/jb.120.1.416-423.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wong C. L., Dunn N. W. Transmissible plasmid coding for the degradation of benzoate and m-toluate in Pseudomonas arvilla mt-2. Genet Res. 1974 Apr;23(2):227–232. doi: 10.1017/s0016672300014853. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES