Abstract
Escherichia coli mutants with alterations in the electrophoretic mobility of ribosomal protein S9 were used to locate rpsI, the gene for this protein, on the linkage map. rpsI was located at about 70 min, roughly halfway between argG and fabE. It was very close to the gene for ribosomal protein L13, rplM. Another mutation at the rpsI locus gave rise to a phenotype of kasugamycin dependence and resistance. In this mutant, dependence on antibiotic came from kasugamycin being necessary to slow the rate of protein synthesis.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bachmann B. J., Low K. B. Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980 Mar;44(1):1–56. doi: 10.1128/mr.44.1.1-56.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dabbs E. R. Kasugamycin-dependent mutants of Escherichia coli. J Bacteriol. 1978 Dec;136(3):994–1001. doi: 10.1128/jb.136.3.994-1001.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dabbs E. R., Looman K. An antibiotic dependent conditional lethal mutant with a lesion affecting transcription and translation. Mol Gen Genet. 1981;184(2):224–229. doi: 10.1007/BF00272909. [DOI] [PubMed] [Google Scholar]
- Dabbs E. R. Mutational alterations in 50 proteins of the Escherichia coli ribosome. Mol Gen Genet. 1978 Sep 20;165(1):73–78. doi: 10.1007/BF00270378. [DOI] [PubMed] [Google Scholar]
- Dabbs E. R., Poldermans B., Bakker H., van Knippenberg P. H. Biochemical characterization of ribosomes of kasugamycin-dependent mutants of Escherichia coli. FEBS Lett. 1980 Aug 11;117(1):164–166. doi: 10.1016/0014-5793(80)80936-7. [DOI] [PubMed] [Google Scholar]
- Dabbs E. R. The gene for ribosomal protein L13, rplM, is located near argR, at about 70 minutes on the Escherichia coli chromosomal linkage map. J Bacteriol. 1982 Feb;149(2):779–782. doi: 10.1128/jb.149.2.779-782.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dabbs E. R. The gene for ribosomal protein S21, rpsU, maps close to dnaG at 66.5 min on the Escherichia coli chromosomal linkage map. J Bacteriol. 1980 Nov;144(2):603–607. doi: 10.1128/jb.144.2.603-607.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kitakawa M., Dabbs E. R., Isono K. Genes coding for ribosomal proteins S15, L21, and L27 map near argG in Escherichia coli. J Bacteriol. 1979 Jun;138(3):832–838. doi: 10.1128/jb.138.3.832-838.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laughrea M., Moore P. B. On the relationship between the binding of ribosomal protein S1 to the 30 S subunit of Escherichia coli and 3' terminus of 16 S RNA. J Mol Biol. 1978 Jun 5;121(4):411–430. doi: 10.1016/0022-2836(78)90391-1. [DOI] [PubMed] [Google Scholar]
- Sillers I. Y., Moore P. B. Position of protein S1 in the 30 S ribosomal subunit of Escherichia coli. J Mol Biol. 1981 Dec 15;153(3):761–780. doi: 10.1016/0022-2836(81)90417-4. [DOI] [PubMed] [Google Scholar]
- Tai P. C., Wallace B. J., Davis B. D. Actions of aurintricarboxylate, kasugamycin, and pactamycin on Escherichia coli polysomes. Biochemistry. 1973 Feb;12(4):616–620. doi: 10.1021/bi00728a008. [DOI] [PubMed] [Google Scholar]