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. 1983 Feb;153(2):716–721. doi: 10.1128/jb.153.2.716-721.1983

Cloning and expression of the cloacin DF13/aerobactin receptor of Escherichia coli (ColV-K30).

W J Krone, B Oudega, F Stegehuis, F K de Graaf
PMCID: PMC221689  PMID: 6218156

Abstract

A DNA fragment derived from the ColV-K30 plasmid and coding for both sensitivity to cloacin DF13 and Fe3+-aerobactin uptake was cloned into pBR322. The cloned fragment coded for two polypeptides with molecular masses of 74,000 (the cloacin DF13/aerobactin receptor protein) and 50,000 daltons, respectively. When grown with sufficient iron, cells harboring pFS8 (with this fragment) possessed about 10 times as many receptor protein molecules as compared with cells of Escherichia coli (ColV-K30). The synthesis of the receptor protein specified by pFS8, however, was independent of the availability of iron, in contrast to strains harboring the intact ColV-K30 plasmid. Aerobactin was taken up but not synthesized by cells harboring pFS8. No growth occurred when iron-starved cultures of these cells were incubated with Fe3+-aerobactin, suggesting that expression of other ColV-K30-encoded genes is necessary to remove the iron from the Fe3+-aerobactin complex.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bindereif A., Braun V., Hantke K. The cloacin receptor of ColV-bearing Escherichia coli is part of the Fe3+-aerobactin transport system. J Bacteriol. 1982 Jun;150(3):1472–1475. doi: 10.1128/jb.150.3.1472-1475.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed] [Google Scholar]
  4. Cohen S. N., Chang A. C., Hsu L. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110–2114. doi: 10.1073/pnas.69.8.2110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dougan G., Sherratt D. The transposon Tn1 as a probe for studying ColE1 structure and function. Mol Gen Genet. 1977 Mar 7;151(2):151–160. doi: 10.1007/BF00338689. [DOI] [PubMed] [Google Scholar]
  6. Grewal K. K., Warner P. J., Williams P. H. An inducible outer membrane protein involved in aerobactin-mediated iron transport by co1V strains of Escherichia coli. FEBS Lett. 1982 Apr 5;140(1):27–30. doi: 10.1016/0014-5793(82)80513-9. [DOI] [PubMed] [Google Scholar]
  7. Hartmann A., Braun V. Iron uptake and iron limited growth of Escherichia coli K-12. Arch Microbiol. 1981 Dec 2;130(5):353–356. doi: 10.1007/BF00414599. [DOI] [PubMed] [Google Scholar]
  8. Kreil G. Transfer of proteins across membranes. Annu Rev Biochem. 1981;50:317–348. doi: 10.1146/annurev.bi.50.070181.001533. [DOI] [PubMed] [Google Scholar]
  9. Mooi F. R., Harms N., Bakker D., de Graaf F. K. Organization and expression of genes involved in the production of the K88ab antigen. Infect Immun. 1981 Jun;32(3):1155–1163. doi: 10.1128/iai.32.3.1155-1163.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Oudega B., Klaasen-Boor P., Sneeuwloper G., De Graaf F. K. Interaction of the complex between cloacin and its immunity protein and of cloacin with the outer and cytoplasmic membranes of sensitive cells. Eur J Biochem. 1977 Sep;78(2):445–453. doi: 10.1111/j.1432-1033.1977.tb11757.x. [DOI] [PubMed] [Google Scholar]
  11. Oudega B., Oldenziel-Werner W. J., Klaasen-Boor P., Rezee A., Glas J., de Graaf F. K. Purification and characterization of cloacin DF13 receptor from Enterobacter cloacae and its interaction with cloacin DF13 in vitro. J Bacteriol. 1979 Apr;138(1):7–16. doi: 10.1128/jb.138.1.7-16.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Oudega B., de Graaf F. K. Enzymatic properties of cloacin DF13 and kinetics of ribosome inactivation. Biochim Biophys Acta. 1976 Mar 17;425(3):296–304. doi: 10.1016/0005-2787(76)90256-2. [DOI] [PubMed] [Google Scholar]
  13. Palva E. T., Hirst T. R., Hardy S. J., Holmgren J., Randall L. Synthesis of a precursor to the B subunit of heat-labile enterotoxin in Escherichia coli. J Bacteriol. 1981 Apr;146(1):325–330. doi: 10.1128/jb.146.1.325-330.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Prakash R. K., Schilperoort R. A., Nuti M. P. Large plasmids of fast-growing rhizobia: homology studies and location of structural nitrogen fixation (nif) genes. J Bacteriol. 1981 Mar;145(3):1129–1136. doi: 10.1128/jb.145.3.1129-1136.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stuart S. J., Greenwood K. T., Luke R. K. Hydroxamate-mediated transport of iron controlled by ColV plasmids. J Bacteriol. 1980 Jul;143(1):35–42. doi: 10.1128/jb.143.1.35-42.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tomoeda M., Inuzuka M., Kubo N., Nakamura S. Effective elimination of drug resistance and sex factors in Escherichia coli by sodium dodecyl sulfate. J Bacteriol. 1968 Mar;95(3):1078–1089. doi: 10.1128/jb.95.3.1078-1089.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Van Tiel-Menkveld G. J., Mentjox-Vervuurt J. M., Oudega B., de Graaf F. K. Siderophore production by Enterobacter cloacae and a common receptor protein for the uptake of aerobactin and cloacin DF13. J Bacteriol. 1982 May;150(2):490–497. doi: 10.1128/jb.150.2.490-497.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wagegg W., Braun V. Ferric citrate transport in Escherichia coli requires outer membrane receptor protein fecA. J Bacteriol. 1981 Jan;145(1):156–163. doi: 10.1128/jb.145.1.156-163.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Warner P. J., Williams P. H., Bindereif A., Neilands J. B. ColV plasmid-specific aerobactin synthesis by invasive strains of Escherichia coli. Infect Immun. 1981 Aug;33(2):540–545. doi: 10.1128/iai.33.2.540-545.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. de Graaf F. K., Niekus H. G., Klootwijk J. Inactivation of bacterial ribosomes in vivo and in vitro by cloacin DF13. FEBS Lett. 1973 Sep 1;35(1):161–165. doi: 10.1016/0014-5793(73)80601-5. [DOI] [PubMed] [Google Scholar]
  21. de Graaf F. K., Spanjaerdt Speckman E. A., Stouthamer A. H. Mode of action of a bacteriocin produced by Enterobacter cloacae DF13. Antonie Van Leeuwenhoek. 1969;35(3):287–306. doi: 10.1007/BF02219150. [DOI] [PubMed] [Google Scholar]
  22. van Tiel-Menkveld G. J., Veltkamp E., De Graaf F. K. Mitomycin C-induced synthesis of cloacin DF13 and lethality in cloacinogenic Escherichia coli cells. J Bacteriol. 1981 Apr;146(1):41–48. doi: 10.1128/jb.146.1.41-48.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

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