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. 1983 Feb;153(2):782–790. doi: 10.1128/jb.153.2.782-790.1983

Long-chain fatty acid assimilation By rhodopseudomonas sphaeroides.

T B Campbell, D R Lueking
PMCID: PMC221697  PMID: 6600450

Abstract

Exogenously supplied long-chain fatty acids have been shown to markedly alleviate the inhibition of phototrophic growth of cultures of Rhodopseudomonas sphaeroides caused by the antibiotic cerulenin. Monounsaturated and polyunsaturated C18 fatty acids were most effective in relieving growth inhibition mediated by cerulenin. Medium supplementation with saturated fatty acids (C14 to C18) failed to influence the inhibitory effect of cerulenin. The addition of mixtures of unsaturated and saturated fatty acids to the growth medium did not enhance the growth of cerulenin-inhibited cultures above that obtained with individual unsaturated fatty acids as supplements. Resolution and fatty acid analysis of the extractable lipids of R. sphaeroides revealed that exogenously supplied fatty acids were directly incorporated into cellular phospholipids. Cells treated with cerulenin displayed an enrichment in their percentage of total saturated fatty acids irrespective of the presence of exogenous fatty acids. Cerulenin produced comparable inhibitions of the rates of both fatty acid and phospholipid synthesis and was further found to preferentially inhibit unsaturated fatty acid synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ames G. F. Lipids of Salmonella typhimurium and Escherichia coli: structure and metabolism. J Bacteriol. 1968 Mar;95(3):833–843. doi: 10.1128/jb.95.3.833-843.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Awaya J., Ohno T., Ohno H., Omura S. Substitution of cellular fatty acids in yeast cells by the antibiotic cerulenin and exogenous fatty acids. Biochim Biophys Acta. 1975 Dec 17;409(3):267–273. doi: 10.1016/0005-2760(75)90022-3. [DOI] [PubMed] [Google Scholar]
  3. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  4. Broglie R. M., Niederman R. A. Membranes of Rhodopseudomonas sphaeroides: effect of cerulenin on assembly of chromatophore membrane. J Bacteriol. 1979 Jun;138(3):788–798. doi: 10.1128/jb.138.3.788-798.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Buttke T. M., Ingram L. O. Inhibition of unsaturated fatty acid synthesis in escherichia coli by the antibiotic cerulenin. Biochemistry. 1978 Nov 28;17(24):5282–5286. doi: 10.1021/bi00617a031. [DOI] [PubMed] [Google Scholar]
  6. Cain B. D., Deal C. D., Fraley R. T., Kaplan S. In vivo intermembrane transfer of phospholipids in the photosynthetic bacterium Rhodopseudomonas sphaeroides. J Bacteriol. 1981 Mar;145(3):1154–1166. doi: 10.1128/jb.145.3.1154-1166.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cohen L. K., Lueking D. R., Kaplan S. Intermembrane phospholipid transfer mediated by cell-free extracts of Rhodopseudomonas sphaeroides. J Biol Chem. 1979 Feb 10;254(3):721–728. [PubMed] [Google Scholar]
  8. Cronan J. E., Jr, Gelmann E. P. Physical properties of membrane lipids: biological relevance and regulation. Bacteriol Rev. 1975 Sep;39(3):232–256. doi: 10.1128/br.39.3.232-256.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Donohue T. J., Cain B. D., Kaplan S. Purification and characterization of an N-acylphosphatidylserine from Rhodopseudomonas sphaeroides. Biochemistry. 1982 May 25;21(11):2765–2773. doi: 10.1021/bi00540a029. [DOI] [PubMed] [Google Scholar]
  10. Drews G., Oelze J. Organization and differentiation of membranes of phototrophic bacteria. Adv Microb Physiol. 1981;22:1–92. doi: 10.1016/s0065-2911(08)60325-2. [DOI] [PubMed] [Google Scholar]
  11. Fraley R. T., Lueking D. R., Kaplan S. The relationship of intracytoplasmic membrane assembly to the cell division cycle in Rhodopseudomonas sphaeroides. J Biol Chem. 1979 Mar 25;254(6):1980–1986. [PubMed] [Google Scholar]
  12. Goldberg I., Walker J. R., Bloch K. Inhibition of lipid synthesis in Escherichia coli cells by the antibiotic cerulenin. Antimicrob Agents Chemother. 1973 May;3(5):549–554. doi: 10.1128/aac.3.5.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Goldfine H., Johnston N. C., Phillips M. C. Phase behavior of ether lipids from Clostridium butyricum. Biochemistry. 1981 May 12;20(10):2908–2916. doi: 10.1021/bi00513a030. [DOI] [PubMed] [Google Scholar]
  14. Gray G. M. Chromatography of lipids. II. The quantitative isolation of the minor (acidic) phospholipids and of phosphatidylethanolamine from the lipid extracts of mammalian tissues. Biochim Biophys Acta. 1967 Dec 5;144(3):519–524. doi: 10.1016/0005-2760(67)90040-9. [DOI] [PubMed] [Google Scholar]
  15. Klein N. C., Mindich L. Isolation and characterization of a glycerol auxotroph of Rhodopseudomonas capsulata: effect of lipid synthesis on the synthesis of photosynthetic pigments. J Bacteriol. 1976 Oct;128(1):337–346. doi: 10.1128/jb.128.1.337-346.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lueking D. R., Campbell T. B., Burghardt R. C. Light-induced division and genomic synchrony in phototrophically growing cultures of Rhodopseudomonas sphaeroides. J Bacteriol. 1981 May;146(2):790–797. doi: 10.1128/jb.146.2.790-797.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lueking D. R., Fraley R. T., Kaplan S. Intracytoplasmic membrane synthesis in synchronous cell populations of Rhodopseudomonas sphaeroides. Fate of "old" and "new" membrane. J Biol Chem. 1978 Jan 25;253(2):451–457. [PubMed] [Google Scholar]
  18. Omura S. The antibiotic cerulenin, a novel tool for biochemistry as an inhibitor of fatty acid synthesis. Bacteriol Rev. 1976 Sep;40(3):681–697. doi: 10.1128/br.40.3.681-697.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Raetz C. R. Enzymology, genetics, and regulation of membrane phospholipid synthesis in Escherichia coli. Microbiol Rev. 1978 Sep;42(3):614–659. doi: 10.1128/mr.42.3.614-659.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rottem S., Markowitz O., Razin S. Cerulenin-induced changes in the lipopolysaccharide content and phospholipid composition of Proteus mirabilis. Eur J Biochem. 1978 Apr 17;85(2):451–456. doi: 10.1111/j.1432-1033.1978.tb12259.x. [DOI] [PubMed] [Google Scholar]
  21. Vance D., Goldberg I., Mitsuhashi O., Bloch K. Inhibition of fatty acid synthetases by the antibiotic cerulenin. Biochem Biophys Res Commun. 1972 Aug 7;48(3):649–656. doi: 10.1016/0006-291x(72)90397-x. [DOI] [PubMed] [Google Scholar]
  22. Wille W., Eisenstadt E., Willecke K. Inhibition of de novo fatty acid synthesis by the antibiotic cerulenin in Bacillus subtilis: effects on citrate-Mg2+ transport and synthesis of macromolecules. Antimicrob Agents Chemother. 1975 Sep;8(3):231–237. doi: 10.1128/aac.8.3.231. [DOI] [PMC free article] [PubMed] [Google Scholar]

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