Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1983 Feb;153(2):852–860. doi: 10.1128/jb.153.2.852-860.1983

Characterization of a conditionally transformation-deficient mutant of Haemophilus influenzae that carries a mutation in the rec-1 gene region.

J Kooistra, T van Boxel, G Venema
PMCID: PMC221705  PMID: 6401707

Abstract

A mutant of Haemophilus influenzae, designated HM5, carrying a mutation in the rec-1 gene region, is described. This mutant transformed approximately 100-fold less well than does the wild type, but approximately 100-fold better than rec1 mutants. The mutant was less sensitive to UV irradiation and less "reckless" than rec1 mutants. In contrast to rec1 lysogens, HP1c1 lysogens of the mutant were inducible, and during transformation, recombinant-type activity was formed to the same extent as in the wild type. Although the integration of donor DNA was complete, the integrated DNA was not replicated at 36 degrees C. Both the inhibition of replication of the donor-recipient DNA complex and the transformation deficiency could be suppressed when, after DNA entry, the cells were incubated under suboptimal conditions. The loss of colony formation after UV irradiation was suppressible by the same conditions.

Full text

PDF
852

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnhart B. J., Cox S. H. Radiation-sensitive and radiation-resistant mutants of Haemophilus influenzae. J Bacteriol. 1968 Jul;96(1):280–282. doi: 10.1128/jb.96.1.280-282.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barnhart D. J., Cox S. H. Recovery of Haemophilus influenzae from ultraviolet and x-ray damage. Photochem Photobiol. 1970 Mar;11(3):147–162. doi: 10.1111/j.1751-1097.1970.tb05983.x. [DOI] [PubMed] [Google Scholar]
  3. Boling M. E., Allison D. P., Setlow J. K. Bacteriophage of Haemophilus influenzae. 3. Morphology, DNA homology, and immunity properties of HPlcl, S2, and the defective bacteriophage from strain Rd. J Virol. 1973 Apr;11(4):585–591. doi: 10.1128/jvi.11.4.585-591.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Caster J. H., Goodgal S. H. Competence Mutant of Haemophilus influenzae with Abnormal Ratios of Marker Efficiencies in Transformation. J Bacteriol. 1972 Oct;112(1):492–502. doi: 10.1128/jb.112.1.492-502.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Caster J. H., Postel E. H., Goodgal S. H. Competence mutants: isolation of transformation deficient strains of Haemophilus influenzae. Nature. 1970 Aug 1;227(5257):515–517. doi: 10.1038/227515a0. [DOI] [PubMed] [Google Scholar]
  6. Kooistra J., Setlow J. K. Similarity in properties and mapping of three Rec mutants of Haemophilus influenzae. J Bacteriol. 1976 Jul;127(1):327–333. doi: 10.1128/jb.127.1.327-333.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kooistra J., Venema G. Fate of donor DNA in some poorly transformable strains of Haemophilus influenzae. Mutat Res. 1970 Mar;9(3):245–253. doi: 10.1016/0027-5107(70)90126-0. [DOI] [PubMed] [Google Scholar]
  8. Kooistra J., Venema G. Fate of donor deoxyribonucleic acid in a highly transformation-deficient strain of Haemophilus influenzae. J Bacteriol. 1974 Sep;119(3):705–717. doi: 10.1128/jb.119.3.705-717.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kooistra J., Venema G. Poor transformability with novr and eryr donor DNA of some mitomycin-C-sensitive strains of Haemophilus influenzae. Mutat Res. 1973 Dec;20(3):313–326. doi: 10.1016/0027-5107(73)90054-7. [DOI] [PubMed] [Google Scholar]
  10. Kooistra J., Venema G. Properties of Haemophilus influenzae mutants that are slightly recombination deficient and carry a mutation in the rec-1 gene region. J Bacteriol. 1980 Jun;142(3):829–835. doi: 10.1128/jb.142.3.829-835.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Notani N., Goodgal S. H. On the nature of recombinants formed during transformation in Hemophilus influenzae. J Gen Physiol. 1966 Jul;49(6):197–209. doi: 10.1085/jgp.49.6.197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Noteborn M., Venema G., Kooistra J. Effect of ethylenediaminetetraacetic acid on deoxyribonucleic acid entry and recombination in transformation of a wild-type strain and a rec-1 mutant of Haemophilus influenzae. J Bacteriol. 1981 Mar;145(3):1189–1195. doi: 10.1128/jb.145.3.1189-1195.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. STUY J. H., STERN D. THE KINETICS OF DNA UPTAKE BY HAEMOPHILUS INFLUENZAE. J Gen Microbiol. 1964 Jun;35:391–400. doi: 10.1099/00221287-35-3-391. [DOI] [PubMed] [Google Scholar]
  14. Sedgwick B., Setlow J. K., Boling M. E., Allison D. P. Minicell production and bacteriophage superinducibility of thymidine-requiring strains of Haemophilus influenzae. J Bacteriol. 1975 Sep;123(3):1208–1217. doi: 10.1128/jb.123.3.1208-1217.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Setlow J. K., Boling M. E., Allison D. P., Beattie K. L. Relationship between prophage induction and transformation in Haemophilus influenzae. J Bacteriol. 1973 Jul;115(1):153–161. doi: 10.1128/jb.115.1.153-161.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Setlow J. K., Boling M. E., Beattie K. L., Kimball R. F. A complex of recombination and repair genes in Haemophilus influenzae. J Mol Biol. 1972 Jul 21;68(2):361–378. doi: 10.1016/0022-2836(72)90218-5. [DOI] [PubMed] [Google Scholar]
  17. Stachura I., Mckinley F. W., Leidy G., Alexander H. E. Incomplete bacteriophage-like particles in ultraviolet-irradiated haemophilus. J Bacteriol. 1969 May;98(2):818–820. doi: 10.1128/jb.98.2.818-820.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stuy J. H. Fate of transforming DNA in the Haemophilus influenzae transformation system. J Mol Biol. 1965 Sep;13(2):554–570. doi: 10.1016/s0022-2836(65)80117-6. [DOI] [PubMed] [Google Scholar]
  19. Voll M. J., Goodgal S. H. Loss of activity of transforming deoxyribonucleic acid after uptake by Haemophilus influenzae. J Bacteriol. 1965 Oct;90(4):873–883. doi: 10.1128/jb.90.4.873-883.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES