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. 1983 Feb;153(2):884–894. doi: 10.1128/jb.153.2.884-894.1983

Tryptophan biosynthesis in the marine luminous bacterium Vibrio harveyi.

C D Bieger, I P Crawford
PMCID: PMC221710  PMID: 6822478

Abstract

Tryptophan biosynthetic enzyme levels in wild-type Vibrio harveyi and a number of tryptophan auxotrophs of this species were coordinately regulated over a 100-fold range of specific activities. The tryptophan analog indoleacrylic acid evoked substantial derepression of the enzymes in wild-type cells. Even higher enzyme levels were attained in auxotrophs starved for tryptophan, regardless of the location of the block in the pathway. A derepressed mutant selected by resistance to 5-fluorotryptophan was found to have elevated basal levels of trp gene expression; these basal levels were increased only two- to threefold by tryptophan limitation. The taxonomic implications of these and other biochemical results support previous suggestions that the marine luminous bacteria are more closely related to enteric bacteria than to other gram-negative taxa.

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Selected References

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  1. Balbinder E., Callahan R., 3rd, McCann P. P., Cordaro J. C., Weber A. R., Smith A. M., Angelosanto F. Regulatory mutants of the tryptophan operon of Salmonella typhimurium. Genetics. 1970 Sep;66(1):31–53. doi: 10.1093/genetics/66.1.31. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baumann L., Baumann P. Regulation of aspartokinase activity in the genus Beneckea and marine, luminous bacteria. Arch Mikrobiol. 1973;90(3):171–188. doi: 10.1007/BF00424970. [DOI] [PubMed] [Google Scholar]
  3. Baumann P., Baumann L. Biology of the marine enterobacteria: genera Beneckea and Photobacterium. Annu Rev Microbiol. 1977;31:39–61. doi: 10.1146/annurev.mi.31.100177.000351. [DOI] [PubMed] [Google Scholar]
  4. Bertrand K., Korn L., Lee F., Platt T., Squires C. L., Squires C., Yanofsky C. New features of the regulation of the tryptophan operon. Science. 1975 Jul 4;189(4196):22–26. doi: 10.1126/science.1094538. [DOI] [PubMed] [Google Scholar]
  5. Cohn W., Crawford I. P. Regulation of enzyme synthesis in the tryptophan pathway of Acinetobacter calcoaceticus. J Bacteriol. 1976 Jul;127(1):367–379. doi: 10.1128/jb.127.1.367-379.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Crawford I. P. Gene rearrangements in the evolution of the tryptophan synthetic pathway. Bacteriol Rev. 1975 Jun;39(2):87–120. doi: 10.1128/br.39.2.87-120.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Crawford I. P., Gunsalus I. C. Inducibility of tryptophan synthetase in Pseudomonas putida. Proc Natl Acad Sci U S A. 1966 Aug;56(2):717–724. doi: 10.1073/pnas.56.2.717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Crawford I. P., Sikes S., Belser N. O., Martinez L. Mutants of Escherichia coli defective in the B protein of tryptophan synthetase. 3. Intragenic clustering. Genetics. 1970 Jun;65(2):201–211. doi: 10.1093/genetics/65.2.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hiraga S. Operator mutants of the tryptophan operon in Escherichia coli. J Mol Biol. 1969 Jan 14;39(1):159–179. doi: 10.1016/0022-2836(69)90340-4. [DOI] [PubMed] [Google Scholar]
  10. Hollis D. G., Weaver R. E., Baker C. N., Thornsberry C. Halophilic Vibrio species isolated from blood cultures. J Clin Microbiol. 1976 Apr;3(4):425–431. doi: 10.1128/jcm.3.4.425-431.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ito J., Crawford I. P. Regulation of the enzymes of the tryptophan pathway in Escherichia coli. Genetics. 1965 Dec;52(6):1303–1316. doi: 10.1093/genetics/52.6.1303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Largen M., Belser W. The apparent conservation of the internal low efficiency promoter of the tryptophan operons of several species of Enterobacteriaceae. Genetics. 1973 Sep;75(1):19–22. doi: 10.1093/genetics/75.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Matchett W. H. Inhibition of tryptophan synthetase by indoleacrylic acid. J Bacteriol. 1972 Apr;110(1):146–154. doi: 10.1128/jb.110.1.146-154.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mosteller R. D., Mandula B. B. Kinetics of derepression of the tryptophan operon of Escherichia coli and Salmonella typhimurium under different culture conditions. J Mol Biol. 1973 Nov 15;80(4):801–823. doi: 10.1016/0022-2836(73)90211-8. [DOI] [PubMed] [Google Scholar]
  15. Reichelt J. L., Baumann P., Baumann L. Study of genetic relationships among marine species of the genera Beneckea and Photobacterium by means of in vitro DNA/DNA hybridization. Arch Microbiol. 1976 Oct 11;110(1):101–120. doi: 10.1007/BF00416975. [DOI] [PubMed] [Google Scholar]
  16. Sanderson K. E. Genetic relatedness in the family Enterobacteriaceae. Annu Rev Microbiol. 1976;30:327–349. doi: 10.1146/annurev.mi.30.100176.001551. [DOI] [PubMed] [Google Scholar]
  17. Sawula R. V., Crawford I. P. Anthranilate synthetase of Acinetobacter calcoaceticus. Separation and partial characterization of subunits. J Biol Chem. 1973 May 25;248(10):3573–3581. [PubMed] [Google Scholar]
  18. Schwartz D. O., Beckwith J. R. Mutagens which cause deletions in Escherichia coli. Genetics. 1969 Feb;61(2):371–376. doi: 10.1093/genetics/61.2.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Woese C. R., Pribula C. D., Fox G. E., Zablen L. B. The nucleotide sequence of the 5S ribosomal RNA from a photobacterium. J Mol Evol. 1975 Jun 9;5(1):35–46. doi: 10.1007/BF01732012. [DOI] [PubMed] [Google Scholar]
  20. Woolkalis M. J., Baumann P. Evolution of alkaline phosphatase in marine species of Vibrio. J Bacteriol. 1981 Jul;147(1):36–45. doi: 10.1128/jb.147.1.36-45.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

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