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. 1983 Feb;153(2):916–920. doi: 10.1128/jb.153.2.916-920.1983

A voltage clamp inhibits chemotaxis of Spirochaeta aurantia.

E A Goulbourne Jr, E P Greenberg
PMCID: PMC221714  PMID: 6822479

Abstract

Anaerobic conditions were employed to study the relationship between membrane potential and chemotaxis in Spirochaeta aurantia. When cells were grown anaerobically and suspended in anaerobic potassium phosphate buffer (pH 5.5), membranes did not appear to be polarized. Nevertheless, motility was supported by a transmembrane pH gradient, and the anaerobic cells exhibited D-xylose taxis. Introduction of trace amounts of air into anaerobic cell suspensions resulted in a transient membrane polarization. The addition of valinomycin to cells suspended under anaerobic conditions did not alter the steady-state value of membrane potential appreciably but served to clamp membrane potential at the preset level. Although there was no detectable effect of valinomycin on the motility of anaerobic cells in potassium phosphate buffer, D-xylose taxis was completely inhibited by this treatment. These data indicate the the action of valinomycin as a voltage clamp serves to inhibit the chemotaxis of S. aurantia and provide evidence to support the suggestion that the mechanism of chemotaxis in this organism involves the transduction of sensory signals in the form of membrane potential fluctuations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler J. A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli. J Gen Microbiol. 1973 Jan;74(1):77–91. doi: 10.1099/00221287-74-1-77. [DOI] [PubMed] [Google Scholar]
  2. Adler J. Chemotaxis in bacteria. Annu Rev Biochem. 1975;44:341–356. doi: 10.1146/annurev.bi.44.070175.002013. [DOI] [PubMed] [Google Scholar]
  3. Adler J. The behavior of bacteria: on the mechanism of sensory transduction in bacterial chemotaxis. Johns Hopkins Med J. 1979 Apr;144(4):121–126. [PubMed] [Google Scholar]
  4. Armitage J. P., Evans M. C. Chemotactically induced increase in the membrane potential of spheroplasts of Rhodopseudomonas sphaeroides. FEBS Lett. 1980 Mar 24;112(1):5–9. doi: 10.1016/0014-5793(80)80113-x. [DOI] [PubMed] [Google Scholar]
  5. Armitage J. P., Evans M. C. Membrane potential changes during chemotaxis of Rhodopseudomonas sphaeroides. FEBS Lett. 1979 Jun 1;102(1):143–146. doi: 10.1016/0014-5793(79)80946-1. [DOI] [PubMed] [Google Scholar]
  6. Black R. A., Hobson A. C., Adler J. Involvement of cyclic GMP in intracellular signaling in the chemotactic response of Escherichia coli. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3879–3883. doi: 10.1073/pnas.77.7.3879. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Breznak J. A., Canale-Parola E. Morphology and physiology of Spirochaeta aurantia strains isolated from aquatic habitats. Arch Microbiol. 1975 Sep 30;105(1):1–12. doi: 10.1007/BF00447104. [DOI] [PubMed] [Google Scholar]
  8. Goulbourne E. A., Jr, Greenberg E. P. Relationship between proton motive force and motility in Spirochaeta aurantia. J Bacteriol. 1980 Sep;143(3):1450–1457. doi: 10.1128/jb.143.3.1450-1457.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Greenberg E. P., Canale-Parola E. Chemotaxis in Spirochaeta aurantia. J Bacteriol. 1977 Apr;130(1):485–494. doi: 10.1128/jb.130.1.485-494.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Harold F. M., Altendorf K. H., Hirata H. Probing membrane transport mechanisms with inophores. Ann N Y Acad Sci. 1974 May 10;235(0):149–160. doi: 10.1111/j.1749-6632.1974.tb43264.x. [DOI] [PubMed] [Google Scholar]
  11. Hazelbauer G. L., Engström P. Parallel pathways for transduction of chemotactic signals in Escherichia coli. Nature. 1980 Jan 3;283(5742):98–100. doi: 10.1038/283098a0. [DOI] [PubMed] [Google Scholar]
  12. Kashket E. R. Effects of aerobiosis and nitrogen source on the proton motive force in growing Escherichia coli and Klebsiella pneumoniae cells. J Bacteriol. 1981 Apr;146(1):377–384. doi: 10.1128/jb.146.1.377-384.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Khan S., Macnab R. M. The steady-state counterclockwise/clockwise ratio of bacterial flagellar motors is regulated by protonmotive force. J Mol Biol. 1980 Apr 15;138(3):563–597. doi: 10.1016/s0022-2836(80)80018-0. [DOI] [PubMed] [Google Scholar]
  14. Kihara M., Macnab R. M. Cytoplasmic pH mediates pH taxis and weak-acid repellent taxis of bacteria. J Bacteriol. 1981 Mar;145(3):1209–1221. doi: 10.1128/jb.145.3.1209-1221.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Laszlo D. J., Taylor B. L. Aerotaxis in Salmonella typhimurium: role of electron transport. J Bacteriol. 1981 Feb;145(2):990–1001. doi: 10.1128/jb.145.2.990-1001.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. MITCHELL P. Coupling of phosphorylation to electron and hydrogen transfer by a chemi-osmotic type of mechanism. Nature. 1961 Jul 8;191:144–148. doi: 10.1038/191144a0. [DOI] [PubMed] [Google Scholar]
  17. Macnab R. M. Bacterial motility and chemotaxis: the molecular biology of a behavioral system. CRC Crit Rev Biochem. 1978;5(4):291–341. doi: 10.3109/10409237809177145. [DOI] [PubMed] [Google Scholar]
  18. Macnab R. M., Koshland D. E., Jr The gradient-sensing mechanism in bacterial chemotaxis. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2509–2512. doi: 10.1073/pnas.69.9.2509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Manson M. D., Tedesco P., Berg H. C., Harold F. M., Van der Drift C. A protonmotive force drives bacterial flagella. Proc Natl Acad Sci U S A. 1977 Jul;74(7):3060–3064. doi: 10.1073/pnas.74.7.3060. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Miller J. B., Koshland D. E., Jr Protonmotive force and bacterial sensing. J Bacteriol. 1980 Jan;141(1):26–32. doi: 10.1128/jb.141.1.26-32.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Miller J. B., Koshland D. E., Jr Sensory electrophysiology of bacteria: relationship of the membrane potential to motility and chemotaxis in Bacillus subtilis. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4752–4756. doi: 10.1073/pnas.74.11.4752. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Mitchell P. Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Biol Rev Camb Philos Soc. 1966 Aug;41(3):445–502. doi: 10.1111/j.1469-185x.1966.tb01501.x. [DOI] [PubMed] [Google Scholar]
  23. Murvanidze G. V., Glagolev A. N. Electrical nature of the taxis signal in cyanobacteria. J Bacteriol. 1982 Apr;150(1):239–244. doi: 10.1128/jb.150.1.239-244.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Ordal G. W. Calcium ion regulates chemotactic behaviour in bacteria. Nature. 1977 Nov 3;270(5632):66–67. doi: 10.1038/270066a0. [DOI] [PubMed] [Google Scholar]
  25. Pressman B. C. Biological applications of ionophores. Annu Rev Biochem. 1976;45:501–530. doi: 10.1146/annurev.bi.45.070176.002441. [DOI] [PubMed] [Google Scholar]
  26. Repaske D. R., Adler J. Change in intracellular pH of Escherichia coli mediates the chemotactic response to certain attractants and repellents. J Bacteriol. 1981 Mar;145(3):1196–1208. doi: 10.1128/jb.145.3.1196-1208.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Snyder M. A., Stock J. B., Koshland D. E., Jr Role of membrane potential and calcium in chemotactic sensing by bacteria. J Mol Biol. 1981 Jun 25;149(2):241–257. doi: 10.1016/0022-2836(81)90300-4. [DOI] [PubMed] [Google Scholar]
  28. Stanton T. B., Canale-Parola E. Enumeration and selective isolation of rumen spirochetes. Appl Environ Microbiol. 1979 Nov;38(5):965–973. doi: 10.1128/aem.38.5.965-973.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Szmelcman S., Adler J. Change in membrane potential during bacterial chemotaxis. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4387–4391. doi: 10.1073/pnas.73.12.4387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Waggoner A. Optical probes of membrane potential. J Membr Biol. 1976 Jun 30;27(4):317–334. doi: 10.1007/BF01869143. [DOI] [PubMed] [Google Scholar]

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