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. 1983 Feb;153(2):991–997. doi: 10.1128/jb.153.2.991-997.1983

Capsular polysaccharides and lipopolysaccharides from two Bacteroides fragilis reference strains: chemical and immunochemical characterization.

D L Kasper, A Weintraub, A A Lindberg, J Lönngren
PMCID: PMC221723  PMID: 6185469

Abstract

Fermentor growth of Bacteroides fragilis under controlled conditions in a complex medium containing 1% glucose and 10% fetal calf serum resulted in high yields of bacteria. After hot phenol-water extraction of the organisms, capsular polysaccharide was isolated from the aqueous phase and purified by Sephacryl S-300 chromatography in a buffer with 3% sodium deoxycholate. Lipopolysaccharide was isolated by phenol-chloroform-light petroleum ether extraction. The capsular polysaccharide from B. fragilis strain NCTC 9343 contained six sugars: L-fucose, D-galactose, D- and L-quinovosamine, D-glucosamine, and galacturonic acid. The capsule of strain ATCC 23745 also contained D-glucose, L-fucosamine, L-rhamnosamine, and a 3-amino-3,6-dideoxyhexose but lacked D-quinovosamine. The latter capsule also contained alanine (4%). The capsular polysaccharides were different immunochemically by ELISA inhibition. The lipopolysaccharide of both strains contained the same sugars (L-rhamnose, D-glucose, D-galactose, and D-glucosamine) and fatty acids (13-methyl-tetradecanoic and 3-hydroxy-hexadecanoic and 3-hydroxy-15 methyl-hexadecanoic as major constituents) and were identical by ELISA inhibition.

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Selected References

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  1. Babb J. L., Cummings C. S. Relationships between serological groups and deoxyribonucleic acid homology groups in Bacteroides fragilis and related species. J Clin Microbiol. 1981 Feb;13(2):369–379. doi: 10.1128/jcm.13.2.369-379.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dmitriev B. A., Knirel Y. A., Kocharova N. A., Kochetkov N. K., Stanislavsky E. S., Mashilova G. M. Somatic antigens of Pseudomonas aeruginosa. The structure of the polysaccharide chain of Ps. aeruginosa O-serogroup 7 (Lanyi) lipopolysacharide. Eur J Biochem. 1980 May;106(2):643–651. doi: 10.1111/j.1432-1033.1980.tb04612.x. [DOI] [PubMed] [Google Scholar]
  3. Galanos C., Lüderitz O., Westphal O. A new method for the extraction of R lipopolysaccharides. Eur J Biochem. 1969 Jun;9(2):245–249. doi: 10.1111/j.1432-1033.1969.tb00601.x. [DOI] [PubMed] [Google Scholar]
  4. Gorbach S. L., Bartlett J. G. Anaerobic infections (second of three parts). N Engl J Med. 1974 May 30;290(22):1237–1245. doi: 10.1056/NEJM197405302902207. [DOI] [PubMed] [Google Scholar]
  5. Hofstad T., Sveen K., Dahlén G. Chemical composition, serological reactivity and endotoxicity of lipopolysaccharides extracted in different ways from Bacteroides fragilis, Bacteroides melaninogenicus and Bacteroides oralis. Acta Pathol Microbiol Scand B. 1977 Aug;85(4):262–270. doi: 10.1111/j.1699-0463.1977.tb01972.x. [DOI] [PubMed] [Google Scholar]
  6. Jann B., Jann K. 2-amino-2,6-dideoxy-L-mannose (L-rhamnosamine) isolated from the lipopolysaccharide of Escherichia coli 03:K2ab(L):H2. Eur J Biochem. 1968 Jul;5(2):173–177. doi: 10.1111/j.1432-1033.1968.tb00354.x. [DOI] [PubMed] [Google Scholar]
  7. Jann B., Jann K., Beyaert G. O. 2-Amino-2,6-dideoxy-d-glucose (D-quinovosamine): a constituent of the lipopolysaccharides of Vibrio cholerae. Eur J Biochem. 1973 Sep 3;37(3):531–534. doi: 10.1111/j.1432-1033.1973.tb03015.x. [DOI] [PubMed] [Google Scholar]
  8. Kasper D. L. Chemical and biological characterization of the lipopolysaccharide of Bacteroides fragilis subspecies fragilis. J Infect Dis. 1976 Jul;134(1):59–66. doi: 10.1093/infdis/134.1.59. [DOI] [PubMed] [Google Scholar]
  9. Kasper D. L., Fiddian A. P., Tabaqchali S. Rapid diagnosis of Bacteroides infections by indirect immunofluorescence assay of clinical specimens. Lancet. 1979 Feb 3;1(8110):239–242. doi: 10.1016/s0140-6736(79)90768-2. [DOI] [PubMed] [Google Scholar]
  10. Kasper D. L., Hayes M. E., Reinap B. G., Craft F. O., Onderdonk A. B., Polk B. F. Isolation and identification of encapsulated strains of Bacteroides fragilis. J Infect Dis. 1977 Jul;136(1):75–81. doi: 10.1093/infdis/136.1.75. [DOI] [PubMed] [Google Scholar]
  11. Kasper D. L., Onderdonk A. B., Crabb J., Bartlett J. G. Protective efficacy of immunization with capsular antigen against experimental infection with Bacteroides fragilis. J Infect Dis. 1979 Nov;140(5):724–731. doi: 10.1093/infdis/140.5.724. [DOI] [PubMed] [Google Scholar]
  12. Kasper D. L., Onderdonk A. B., Polk B. F., Bartlett J. G. Surface antigens as virulence factors in infection with Bacteroides fragilis. Rev Infect Dis. 1979 Mar-Apr;1(2):278–290. doi: 10.1093/clinids/1.2.278. [DOI] [PubMed] [Google Scholar]
  13. Kasper D. L., Onderdonk A. B., Reinap B. G., Linberg A. A. Variations of Bacteroides fragilis with in vitro passage: presence of an outer membrane-associated glycan and loss of capsular antigen. J Infect Dis. 1980 Nov;142(5):750–756. doi: 10.1093/infdis/142.5.750. [DOI] [PubMed] [Google Scholar]
  14. Kasper D. L., Seiler M. W. Immunochemical characterization of the outer membrane complex of Bacteroides fragilis subspecies fragilis. J Infect Dis. 1975 Oct;132(4):440–450. doi: 10.1093/infdis/132.4.440. [DOI] [PubMed] [Google Scholar]
  15. Kasper D. L. The polysaccharide capsule of Bacteroides fragilis subspecies fragilis: immunochemical and morphologic definition. J Infect Dis. 1976 Jan;133(1):79–87. doi: 10.1093/infdis/133.1.79. [DOI] [PubMed] [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  17. Lindberg A. A., Weintraub A., Nord C. E. The humoral antibody response to Bacteroides fragilis infections in humans. Scand J Infect Dis Suppl. 1979;(19):46–51. [PubMed] [Google Scholar]
  18. Onderdonk A. B., Markham R. B., Zaleznik D. F., Cisneros R. L., Kasper D. L. Evidence for T cell-dependent immunity to Bacteroides fragilis in an intraabdominal abscess model. J Clin Invest. 1982 Jan;69(1):9–16. doi: 10.1172/JCI110445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Polk B. F., Kasper D. L. Bacteroides fragilis subspecies in clinical isolates. Ann Intern Med. 1977 May;86(5):569–571. doi: 10.7326/0003-4819-86-5-569. [DOI] [PubMed] [Google Scholar]
  20. Schleifer K. H., Kandler O. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev. 1972 Dec;36(4):407–477. doi: 10.1128/br.36.4.407-477.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shapiro M. E., Onderdonk A. B., Kasper D. L., Finberg R. W. Cellular immunity to Bacteroides fragilis capsular polysaccharide. J Exp Med. 1982 Apr 1;155(4):1188–1197. doi: 10.1084/jem.155.4.1188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Taylor R. L., Conrad H. E. Stoichiometric depolymerization of polyuronides and glycosaminoglycuronans to monosaccharides following reduction of their carbodiimide-activated carboxyl groups. Biochemistry. 1972 Apr 11;11(8):1383–1388. doi: 10.1021/bi00758a009. [DOI] [PubMed] [Google Scholar]
  23. Weintraub A., Lindberg A. A., Nord C. E. Identification of Bacteroides fragilis by indirect immunofluorescence. Med Microbiol Immunol. 1979;167(4):223–230. doi: 10.1007/BF02120807. [DOI] [PubMed] [Google Scholar]
  24. Wollenweber H. W., Rietschel E. T., Hofstad T., Weintraub A., Lindberg A. A. Nature, type of linkage, quantity, and absolute configuration of (3-hydroxy) fatty acids in lipopolysaccharides from Bacteroides fragilis NCTC 9343 and related strains. J Bacteriol. 1980 Dec;144(3):898–903. doi: 10.1128/jb.144.3.898-903.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

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