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. 1983 Feb;153(2):1008–1017. doi: 10.1128/jb.153.2.1008-1017.1983

Ordering of the flagellar genes in Pseudomonas aeruginosa by insertions of mercury transposon Tn501.

M Tsuda, T Iino
PMCID: PMC221725  PMID: 6296037

Abstract

The flagellar genes of Pseudomonas aeruginosa PAO cluster on the chromosome at two distinct regions, region I and region II. The order of the flagellar cistrons in this organism was established by using transducing phage G101 and plasmids FP5 and R68.45. A method to insert transposon Tn501 near the fla genes was devised. We obtained two strains in which Tn501 was inserted at sites close to the flagellar cistrons in region II. We isolated Fla mutants in which the chromosomal segment between the two Tn501 insertion sites was deleted. Using Tn501-encoded mercury resistance as an outside marker, we determined the order of 9 of the 11 flagellar cistrons in region II as follows: puuF-region I-flaG-flaC-flaI-flaH-flaD-flaB-flaA-flaF-flaE-pur-67. By using phage G101-mediated transduction, the mutation converting monoflagellated bacteria into the multiflagellated (mfl) form was closely linked to the five fla cistrons in region I. Using mfl as an outside marker, we determined the order of the five cistrons as follows: puuF-flaV-flaZ-flaW-flaX-flaY-region II. The mfl mutation was shown to be either located within the flaV cistron or linked very closely to this cistron. No linkage was observed in transductions between any of the fla cistrons in region I and any of the fla cistrons in region II.

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Selected References

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  1. Abstracts of papers presented at the 1980 meetings of the Genetic Society of America. Boulder, Colorado August 18-20, 1980. Genetics. 1980;94(4 Pt 2 Suppl):1–16. [PMC free article] [PubMed] [Google Scholar]
  2. Bennett P. M., Grinsted J., Choi C. L., Richmond M. H. Characterisation of Tn501, a transposon determining resistance to mercuric ions. Mol Gen Genet. 1978 Feb 7;159(1):101–106. doi: 10.1007/BF00401753. [DOI] [PubMed] [Google Scholar]
  3. Campbell A., Starlinger P., Berg D. E., Botstein D., Lederberg E. M., Novick R. P., Szybalski W. Nomenclature of transposable elements in prokaryotes. Plasmid. 1979 Jul;2(3):466–473. doi: 10.1016/0147-619x(79)90030-1. [DOI] [PubMed] [Google Scholar]
  4. Chandler P. M., Krishnapillai V. Characterization of Pseudomonas aeruginosa derepressed R-plasmids. J Bacteriol. 1977 May;130(2):596–603. doi: 10.1128/jb.130.2.596-603.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Datta N., Hedges R. W., Shaw E. J., Sykes R. B., Richmond M. H. Properties of an R factor from Pseudomonas aeruginosa. J Bacteriol. 1971 Dec;108(3):1244–1249. doi: 10.1128/jb.108.3.1244-1249.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fennewald M., Benson S., Oppici M., Shapiro J. Insertion element analysis and mapping of the Pseudomonas plasmid alk regulon. J Bacteriol. 1979 Sep;139(3):940–952. doi: 10.1128/jb.139.3.940-952.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Haas D., Holloway B. W. R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa. Mol Gen Genet. 1976 Mar 30;144(3):243–251. doi: 10.1007/BF00341722. [DOI] [PubMed] [Google Scholar]
  8. Haas D., Watson J., Krieg R., Leisinger T. Isolation of an Hfr donor of Pseudomonas aeruginosa PAO by insertion of the plasmid RP1 into the tryptophan synthase gene. Mol Gen Genet. 1981;182(2):240–244. doi: 10.1007/BF00269664. [DOI] [PubMed] [Google Scholar]
  9. Holloway B. W. Genetics of Pseudomonas. Bacteriol Rev. 1969 Sep;33(3):419–443. doi: 10.1128/br.33.3.419-443.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Holloway B. W. Isolation and characterization of an R' plasmid in Pseudomonas aeruginosa. J Bacteriol. 1978 Mar;133(3):1078–1082. doi: 10.1128/jb.133.3.1078-1082.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Holloway B. W., Krishnapillai V., Morgan A. F. Chromosomal genetics of Pseudomonas. Microbiol Rev. 1979 Mar;43(1):73–102. doi: 10.1128/mr.43.1.73-102.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Iino T. Genetics of structure and function of bacterial flagella. Annu Rev Genet. 1977;11:161–182. doi: 10.1146/annurev.ge.11.120177.001113. [DOI] [PubMed] [Google Scholar]
  13. Komeda Y., Kutsukake K., Iino T. Definition of additional flagellar genes in Escherichia coli K12. Genetics. 1980 Feb;94(2):277–290. doi: 10.1093/genetics/94.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Krishnapillai V. A novel transducing phage. Its role in recognition of a possible new host-controlled modification system in Pseudomonas aeruginosa. Mol Gen Genet. 1972;114(2):134–143. doi: 10.1007/BF00332784. [DOI] [PubMed] [Google Scholar]
  15. Krishnapillai V. DNA insertion mutagenesis in a Pseudomonas aeruginosa R plasmid. Plasmid. 1979 Apr;2(2):237–246. doi: 10.1016/0147-619x(79)90042-8. [DOI] [PubMed] [Google Scholar]
  16. Kutsukake K., Iino T., Komeda Y., Yamaguchi S. Functional homology of fla genes between Salmonella typhimurium and Escherichia coli. Mol Gen Genet. 1980 Apr;178(1):59–67. doi: 10.1007/BF00267213. [DOI] [PubMed] [Google Scholar]
  17. Matsumoto H., Tazaki T. FP5 factor, an undescribed sex factor of Pseudomonas aeruginosa. Jpn J Microbiol. 1973 Sep;17(5):409–417. doi: 10.1111/j.1348-0421.1973.tb00792.x. [DOI] [PubMed] [Google Scholar]
  18. Morgan A. F. Transduction of Pseudomonas aeruginosa with a mutant of bacteriophage E79. J Bacteriol. 1979 Jul;139(1):137–140. doi: 10.1128/jb.139.1.137-140.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Novick R. P., Clowes R. C., Cohen S. N., Curtiss R., 3rd, Datta N., Falkow S. Uniform nomenclature for bacterial plasmids: a proposal. Bacteriol Rev. 1976 Mar;40(1):168–189. doi: 10.1128/br.40.1.168-189.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Royle P. L., Matsumoto H., Holloway B. W. Genetic circularity of the Pseudomonas aeruginosa PAO chromosome. J Bacteriol. 1981 Jan;145(1):145–155. doi: 10.1128/jb.145.1.145-155.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Silverman M., Simon M. I. Bacterial flagella. Annu Rev Microbiol. 1977;31:397–419. doi: 10.1146/annurev.mi.31.100177.002145. [DOI] [PubMed] [Google Scholar]
  22. Stanisich V. A., Bennett P. M., Richmond M. H. Characterization of a translocation unit encoding resistance to mercuric ions that occurs on a nonconjugative plasmid in Pseudomonas aeruginosa. J Bacteriol. 1977 Mar;129(3):1227–1233. doi: 10.1128/jb.129.3.1227-1233.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Stanisich V. A. Interaction between an R factor and an element conferring resistance to mercuric ions in Pseudomonas aeruginosa. Mol Gen Genet. 1974 Feb 6;128(3):201–212. doi: 10.1007/BF00267109. [DOI] [PubMed] [Google Scholar]
  24. Suzuki T., Iino T. Isolation and characterization of multiflagellate mutants of Pseudomonas aeruginosa. J Bacteriol. 1980 Sep;143(3):1471–1479. doi: 10.1128/jb.143.3.1471-1479.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tsuda M., Iino T. Transductional analysis of the flagellar genes in Pseudomonas aeruginosa. J Bacteriol. 1983 Feb;153(2):1018–1026. doi: 10.1128/jb.153.2.1018-1026.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tsuda M., Oguchi T., Iino T. Analysis of flagellar genes in Pseudomonas aeruginosa by use of Rfla plasmids and conjugations. J Bacteriol. 1981 Sep;147(3):1008–1014. doi: 10.1128/jb.147.3.1008-1014.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

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