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. 1983 Feb;153(2):1018–1026. doi: 10.1128/jb.153.2.1018-1026.1983

Transductional analysis of the flagellar genes in Pseudomonas aeruginosa.

M Tsuda, T Iino
PMCID: PMC221726  PMID: 6401701

Abstract

Complementation in bacteriophage E79 tv-l-mediated transduction and the phenotypic properties of the flagellar genes in Pseudomonas aeruginosa PAO were investigated by using 195 flagellar mutants of this organism. A total of 15 fla. 1 mot, and 2 che cistrons were identified. At least 5 fla cistrons (fla V to flaZ) and one mot cistron resided in one region, and at least 10 fla cistrons (flaA to flaJ) and two che cistrons (cheA and cheB) resided in another. The flaC mutants exhibited cistron-specific leakiness on motility agar plates. The flaE cistron may be the structural gene for the component protein of the flagellar filament. The cheA mutations, which resulted in pleiotropic phenotypes for flagellar formation, motility, and taxis, belonged to the same complementation group as the flaF mutations; that is, we inferred that cheA and flaF are synonymous.

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Selected References

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  1. Armstrong J. B., Adler J. Genetics of motility in Escherichia coli: complementation of paralysed mutants. Genetics. 1967 Jul;56(3):363–373. doi: 10.1093/genetics/56.3.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bachmann B. J., Low K. B. Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980 Mar;44(1):1–56. doi: 10.1128/mr.44.1.1-56.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berg H. C. Bacterial behaviour. Nature. 1975 Apr 3;254(5499):389–392. doi: 10.1038/254389a0. [DOI] [PubMed] [Google Scholar]
  4. Calhoun D. H., Pierson D. L., Jensen R. A. The regulation of tryptophan biosynthesis in Pseudomonas aeruginosa. Mol Gen Genet. 1973 Mar 1;121(2):117–132. doi: 10.1007/BF00277526. [DOI] [PubMed] [Google Scholar]
  5. Chandler P. M., Krishnapillai V. Isolation and properties of recombination-deficient mutants of Pseudomonas aeruginosa. Mutat Res. 1974 Apr;23(1):15–23. doi: 10.1016/0027-5107(74)90155-9. [DOI] [PubMed] [Google Scholar]
  6. Holloway B. W. Genetics of Pseudomonas. Bacteriol Rev. 1969 Sep;33(3):419–443. doi: 10.1128/br.33.3.419-443.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Holloway B. W., Krishnapillai V., Morgan A. F. Chromosomal genetics of Pseudomonas. Microbiol Rev. 1979 Mar;43(1):73–102. doi: 10.1128/mr.43.1.73-102.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Iino T. Genetics of structure and function of bacterial flagella. Annu Rev Genet. 1977;11:161–182. doi: 10.1146/annurev.ge.11.120177.001113. [DOI] [PubMed] [Google Scholar]
  9. Joys T. M., Stocker B. A. Complementation of non-flagellate Salmonella mutants. J Gen Microbiol. 1965 Oct;41(1):47–55. doi: 10.1099/00221287-41-1-47. [DOI] [PubMed] [Google Scholar]
  10. Komeda Y., Kutsukake K., Iino T. Definition of additional flagellar genes in Escherichia coli K12. Genetics. 1980 Feb;94(2):277–290. doi: 10.1093/genetics/94.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Krishnapillai V. A novel transducing phage. Its role in recognition of a possible new host-controlled modification system in Pseudomonas aeruginosa. Mol Gen Genet. 1972;114(2):134–143. doi: 10.1007/BF00332784. [DOI] [PubMed] [Google Scholar]
  12. Kutsukake K., Iino T., Komeda Y., Yamaguchi S. Functional homology of fla genes between Salmonella typhimurium and Escherichia coli. Mol Gen Genet. 1980 Apr;178(1):59–67. doi: 10.1007/BF00267213. [DOI] [PubMed] [Google Scholar]
  13. Lederberg J. Linear Inheritance in Transductional Clones. Genetics. 1956 Nov;41(6):845–871. doi: 10.1093/genetics/41.6.845. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Manch J. N., Crawford I. P. Genetic evidence for a positive-acting regulatory factor mediating induction in the tryptophan pathway of Pseudomonas aeruginosa. J Mol Biol. 1982 Mar 25;156(1):67–77. doi: 10.1016/0022-2836(82)90459-4. [DOI] [PubMed] [Google Scholar]
  15. Morgan A. F. Transduction of Pseudomonas aeruginosa with a mutant of bacteriophage E79. J Bacteriol. 1979 Jul;139(1):137–140. doi: 10.1128/jb.139.1.137-140.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Royle P. L., Matsumoto H., Holloway B. W. Genetic circularity of the Pseudomonas aeruginosa PAO chromosome. J Bacteriol. 1981 Jan;145(1):145–155. doi: 10.1128/jb.145.1.145-155.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. STOCKER B. A. Abortive transduction of motility in Salmonella; a nonreplicated gene transmitted through many generations to a single descendant. J Gen Microbiol. 1956 Dec;15(3):575–598. doi: 10.1099/00221287-15-3-575. [DOI] [PubMed] [Google Scholar]
  18. Sanderson K. E., Hartman P. E. Linkage map of Salmonella typhimurium, edition V. Microbiol Rev. 1978 Jun;42(2):471–519. doi: 10.1128/mr.42.2.471-519.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Silverman M., Simon M. I. Bacterial flagella. Annu Rev Microbiol. 1977;31:397–419. doi: 10.1146/annurev.mi.31.100177.002145. [DOI] [PubMed] [Google Scholar]
  20. Silverman M., Simon M. Assembly of hybrid flagellar filaments. J Bacteriol. 1974 May;118(2):750–752. doi: 10.1128/jb.118.2.750-752.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Stanisich V. A. The properties and host range of male-specific bacteriophages of Pseudomonas aeruginosa. J Gen Microbiol. 1974 Oct;84(2):332–342. doi: 10.1099/00221287-84-2-332. [DOI] [PubMed] [Google Scholar]
  22. Suzuki T., Iino T. Appearance of straight flagellar filaments in the presence of p-fluorophenylalanine in Pseudomonas aeruginosa. J Bacteriol. 1977 Jan;129(1):527–529. doi: 10.1128/jb.129.1.527-529.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Suzuki T., Iino T. Isolation and characterization of multiflagellate mutants of Pseudomonas aeruginosa. J Bacteriol. 1980 Sep;143(3):1471–1479. doi: 10.1128/jb.143.3.1471-1479.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Taylor B. L., Koshland D. E., Jr Reversal of flagellar rotation in monotrichous and peritrichous bacteria: generation of changes in direction. J Bacteriol. 1974 Aug;119(2):640–642. doi: 10.1128/jb.119.2.640-642.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tsuda M., Iino T. Ordering of the flagellar genes in Pseudomonas aeruginosa by insertions of mercury transposon Tn501. J Bacteriol. 1983 Feb;153(2):1008–1017. doi: 10.1128/jb.153.2.1008-1017.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tsuda M., Oguchi T., Iino T. Analysis of flagellar genes in Pseudomonas aeruginosa by use of Rfla plasmids and conjugations. J Bacteriol. 1981 Sep;147(3):1008–1014. doi: 10.1128/jb.147.3.1008-1014.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Yamaguchi S., Iino T., Horiguchi T., Ota K. Genetic analysis of fla and mot cistrons closely linked to H1 in Salmonella abortusequi and its derivatives. J Gen Microbiol. 1972 Apr;70(1):59–75. doi: 10.1099/00221287-70-1-59. [DOI] [PubMed] [Google Scholar]

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