Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1983 Feb;153(2):1072–1074. doi: 10.1128/jb.153.2.1072-1074.1983

Role of the sfiA-dependent cell division regulation system in Escherichia coli.

O Huisman, M Jacques, R D'ari, L Caro
PMCID: PMC221736  PMID: 6296040

Abstract

Several authors have suggested that the SOS-associated (sfiA-dependent) system of division inhibition, normally induced by perturbations of DNA replication, also regulates steady-state (unperturbed) cell division. The present work shows that mean cell mass is identical in sfiA+ and sfiA mutant cultures during steady-state growth, that mass adjustment is identical after shift up, that sfiA expression is not induced by shift up, and that a sfiA mutation does not cause aberrant chromosome segregation.

Full text

PDF
1072

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CARO L. G., VAN TUBERGEN R. P., KOLB J. A. High-resolution autoradiography. I. Methods. J Cell Biol. 1962 Nov;15:173–188. doi: 10.1083/jcb.15.2.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Capaldo F. N., Barbour S. D. DNA content, synthesis and integrity in dividing and non-dividing cells of rec- strains of Escherichia coli K12. J Mol Biol. 1975 Jan 5;91(1):53–66. doi: 10.1016/0022-2836(75)90371-x. [DOI] [PubMed] [Google Scholar]
  3. Castellazzi M. Deoxyribonucleic acid degradation in vivo and in permeabilized Escherichia coli repair-deficient (recA zab lexA) derivatives. J Bacteriol. 1976 Sep;127(3):1150–1156. doi: 10.1128/jb.127.3.1150-1156.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clark D. J. Regulation of deoxyribonucleic acid replication and cell division in Escherichia coli B-r. J Bacteriol. 1968 Oct;96(4):1214–1224. doi: 10.1128/jb.96.4.1214-1224.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cohen S. S., Barner H. D. STUDIES ON UNBALANCED GROWTH IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1954 Oct;40(10):885–893. doi: 10.1073/pnas.40.10.885. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. D'Ari R., Huisman O. DNA replication and indirect induction of the SOS response in Escherichia coli. Biochimie. 1982 Aug-Sep;64(8-9):623–627. doi: 10.1016/s0300-9084(82)80100-4. [DOI] [PubMed] [Google Scholar]
  7. Donachie W. D. Control of cell division in Escherichia coli: experiments with thymine starvation. J Bacteriol. 1969 Oct;100(1):260–268. doi: 10.1128/jb.100.1.260-268.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. George J., Castellazzi M., Buttin G. Prophage induction and cell division in E. coli. III. Mutations sfiA and sfiB restore division in tif and lon strains and permit the expression of mutator properties of tif. Mol Gen Genet. 1975 Oct 22;140(4):309–332. [PubMed] [Google Scholar]
  9. Gross J. D., Caro L. G. DNA transfer in bacterial conjugation. J Mol Biol. 1966 Apr;16(2):269–284. doi: 10.1016/s0022-2836(66)80172-9. [DOI] [PubMed] [Google Scholar]
  10. HOWARD-FLANDERS P., SIMSON E., THERIOT L. A LOCUS THAT CONTROLS FILAMENT FORMATION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI K-12. Genetics. 1964 Feb;49:237–246. doi: 10.1093/genetics/49.2.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Helmstetter C. E., Pierucci O. Cell division during inhibition of deoxyribonucleic acid synthesis in Escherichia coli. J Bacteriol. 1968 May;95(5):1627–1633. doi: 10.1128/jb.95.5.1627-1633.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Howe W. E., Mount D. W. Analysis of cell division in single clones of the Escherichia coli K-12 lexA mutant. J Bacteriol. 1978 Mar;133(3):1278–1281. doi: 10.1128/jb.133.3.1278-1281.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Howe W. E., Mount D. W. Distribution of cell lengths in cultures of a lexA mutant of Escherichia coli K-12. J Bacteriol. 1979 Apr;138(1):273–274. doi: 10.1128/jb.138.1.273-274.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Howe W. E., Mount D. W. Production of cells without deoxyribonucleic acid during thymidine starvation of lexA- cultures of Escherichia coli K-12. J Bacteriol. 1975 Dec;124(3):1113–1121. doi: 10.1128/jb.124.3.1113-1121.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Inouye M. Pleiotropic effect of the rec A gene of Escherichia coli: uncoupling of cell division from deoxyribonucleic acid replication. J Bacteriol. 1971 May;106(2):539–542. doi: 10.1128/jb.106.2.539-542.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Little J. W., Mount D. W. The SOS regulatory system of Escherichia coli. Cell. 1982 May;29(1):11–22. doi: 10.1016/0092-8674(82)90085-x. [DOI] [PubMed] [Google Scholar]
  17. Marsden H. S., Pollard E. C., Ginoza W., Randall E. P. Involvement of recA and exr genes in the in vivo inhibition of the recBC nuclease. J Bacteriol. 1974 May;118(2):465–470. doi: 10.1128/jb.118.2.465-470.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Walker J. R., Smith J. A. Cell division of the Escherichia coli lon- mutant. Mol Gen Genet. 1970;108(3):249–257. doi: 10.1007/BF00283355. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES