Abstract
Two internal promoters in the his operon of Salmonella typhimurium have been precisely mapped genetically. The internal promoters are found in, or very close to, gene border regions in the his operon. The his operon was examined for the presence of additional internal promoters whose transcripts were sensitive to rho-mediated transcription termination and therefore had escaped detection. No new internal promoters were found. It is argued that the internal promoters described here are not likely to be fortuitous message start sites, but may play a physiologically important role in operon expression.
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- Artz S. W., Broach J. R. Histidine regulation in Salmonella typhimurium: an activator attenuator model of gene regulation. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3453–3457. doi: 10.1073/pnas.72.9.3453. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Atkins J. F., Loper J. C. Transcription initiation in the histidine operon of Salmonella typhimurium. Proc Natl Acad Sci U S A. 1970 Apr;65(4):925–932. doi: 10.1073/pnas.65.4.925. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ciampi M. S., Schmid M. B., Roth J. R. Transposon Tn10 provides a promoter for transcription of adjacent sequences. Proc Natl Acad Sci U S A. 1982 Aug;79(16):5016–5020. doi: 10.1073/pnas.79.16.5016. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Crawford I. P., Nichols B. P., Yanofsky C. Nucleotide sequence of the trpB gene in Escherichia coli and Salmonella typhimurium. J Mol Biol. 1980 Oct 5;142(4):489–502. doi: 10.1016/0022-2836(80)90259-4. [DOI] [PubMed] [Google Scholar]
- Cunin R., Elseviers D., Sand G., Freundlich G., Glandsdorff N. On the functional organization of the arg ECBH cluster of genes in Escherichia coli K-12. Mol Gen Genet. 1969;106(1):32–47. doi: 10.1007/BF00332819. [DOI] [PubMed] [Google Scholar]
- Ely B., Ciesla Z. Internal promoter P2 of the histidine operon of Salmonella typhimurium. J Bacteriol. 1974 Nov;120(2):984–986. doi: 10.1128/jb.120.2.984-986.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fukumaki Y., Shimada K., Takagi Y. Secondary promoter of the guanine operon of Escherichia coli K-12. J Bacteriol. 1977 Aug;131(2):685–688. doi: 10.1128/jb.131.2.685-688.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grisolia V., Carlomagno M. S., Bruni C. B. Cloning and expression of the distal portion of the histidine operon of Escherichia coli K-12. J Bacteriol. 1982 Aug;151(2):692–700. doi: 10.1128/jb.151.2.692-700.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hartman P. E., Hartman Z., Stahl R. C. Classification and mapping of spontaneous and induced mutations in the histidine operon of Salmonella. Adv Genet. 1971;16:1–34. doi: 10.1016/s0065-2660(08)60352-1. [DOI] [PubMed] [Google Scholar]
- Housley P. R., Leavitt A. D., Whitfield H. J. Genetic analysis of a temperature-sensitive Salmonella typhimurium rho mutant with an altered rho-associated polycytidylate-dependent adenosine triphosphatase activity. J Bacteriol. 1981 Jul;147(1):13–24. doi: 10.1128/jb.147.1.13-24.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Houston L. L. Specialized subregions of the bifunctional hisB gene of Salmonella typhimurium. J Bacteriol. 1973 Jan;113(1):82–87. doi: 10.1128/jb.113.1.82-87.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jackson E. N., Yanofsky C. Internal promoter of the tryptophan operon of Escherichia coli is located in a structural gene. J Mol Biol. 1972 Aug 21;69(2):307–313. doi: 10.1016/0022-2836(72)90232-x. [DOI] [PubMed] [Google Scholar]
- Johnston H. M., Barnes W. M., Chumley F. G., Bossi L., Roth J. R. Model for regulation of the histidine operon of Salmonella. Proc Natl Acad Sci U S A. 1980 Jan;77(1):508–512. doi: 10.1073/pnas.77.1.508. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johnston H. M., Roth J. R. Genetic analysis of the histidine operon control region of Salmonella typhimurium. J Mol Biol. 1981 Feb 5;145(4):713–734. doi: 10.1016/0022-2836(81)90311-9. [DOI] [PubMed] [Google Scholar]
- Kasai T. Regulation of the expression of the histidine operon in Salmonella typhimurium. Nature. 1974 Jun 7;249(457):523–527. doi: 10.1038/249523a0. [DOI] [PubMed] [Google Scholar]
- Kier L. D., Weppelman R. M., Ames B. N. Regulation of nonspecific acid phosphatase in Salmonella: phoN and phoP genes. J Bacteriol. 1979 Apr;138(1):155–161. doi: 10.1128/jb.138.1.155-161.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kleckner N., Chan R. K., Tye B. K., Botstein D. Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition. J Mol Biol. 1975 Oct 5;97(4):561–575. doi: 10.1016/s0022-2836(75)80059-3. [DOI] [PubMed] [Google Scholar]
- Kleckner N., Steele D. A., Reichardt K., Botstein D. Specificity of insertion by the translocatable tetracycline-resistance element Tn10. Genetics. 1979 Aug;92(4):1023–1040. doi: 10.1093/genetics/92.4.1023. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nichols B. P., Yanofsky C. Nucleotide sequences of trpA of Salmonella typhimurium and Escherichia coli: an evolutionary comparison. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5244–5248. doi: 10.1073/pnas.76.10.5244. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Winkler M. E., Roth D. J., Hartman P. E. Promoter- and attenuator-related metabolic regulation of the Salmonella typhimurium histidine operon. J Bacteriol. 1978 Feb;133(2):830–843. doi: 10.1128/jb.133.2.830-843.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]