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. 1983 Mar;153(3):1125–1132. doi: 10.1128/jb.153.3.1125-1132.1983

Mitochondrial translation products during release from glucose repression in Saccharomyces cerevisiae.

C Falcone, M Agostinelli, L Frontali
PMCID: PMC221754  PMID: 6337990

Abstract

Mitochondrial protein synthesis was studied during release from glucose repression in Saccharomyces cerevisiae cells bearing different mitochondrial genomes. The increase in the rate of the synthesis of mitochondrial translation products was analyzed during respiratory induction. Different kinetic patterns were found for strains having a different structure of mitochondrial mosaic genes, even when the nuclear background was the same. A very limited response of the synthesis of the var1 ribosomal protein to inducing conditions was observed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander N. J., Vincent R. D., Perlman P. S., Miller D. H., Hanson D. K., Mahler H. R. Regulatory interactions between mitochondrial genes. I. Genetic and biochemical characterization of some mutant types affecting apocytochrome b and cytochrome oxidase. J Biol Chem. 1979 Apr 10;254(7):2471–2479. [PubMed] [Google Scholar]
  2. Baldacci G., Falcone C., Francisci S., Frontali L., Palleschi C. Mitochondrial protein-synthesizing machinery in Saccharomyces cerevisiae grown in different metabolic conditions. Variability of seryl-tRNA and alanyl-tRNA isoacceptor patterns. Eur J Biochem. 1979 Jul;98(1):181–186. doi: 10.1111/j.1432-1033.1979.tb13175.x. [DOI] [PubMed] [Google Scholar]
  3. Borst P., Grivell L. A. One gene's intron is another gene's exon. Nature. 1981 Feb 5;289(5797):439–440. doi: 10.1038/289439a0. [DOI] [PubMed] [Google Scholar]
  4. Borst P., Grivell L. A. The mitochondrial genome of yeast. Cell. 1978 Nov;15(3):705–723. doi: 10.1016/0092-8674(78)90257-x. [DOI] [PubMed] [Google Scholar]
  5. Chandrasekaran K., Dharmalingam K., Jayaraman J. Assembly of mitochondria in yeast. Complementation of mitochondrial and cytosolic products in a temporal sequence in vitro. Eur J Biochem. 1980 Feb;103(3):471–480. doi: 10.1111/j.1432-1033.1980.tb05971.x. [DOI] [PubMed] [Google Scholar]
  6. Church G. M., Slonimski P. P., Gilbert W. Pleiotropic mutations within two yeast mitochondrial cytochrome genes block mRNA processing. Cell. 1979 Dec;18(4):1209–1215. doi: 10.1016/0092-8674(79)90233-2. [DOI] [PubMed] [Google Scholar]
  7. Dhawale S., Hanson D. K., Alexander N. J., Perlman P. S., Mahler H. R. Regulatory interactions between mitochondrial genes: interactions between two mosaic genes. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1778–1782. doi: 10.1073/pnas.78.3.1778. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Douglas M., Finkelstein D., Butow R. A. Analysis of products of mitochondrial protein synthesis in yeast: genetic and biochemical aspects. Methods Enzymol. 1979;56:58–66. doi: 10.1016/0076-6879(79)56009-1. [DOI] [PubMed] [Google Scholar]
  9. HALVORSON H., FRY W., SCHWEMMIN D. A study of the properties of the free amino acid pool and enzyme synthesis in yeast. J Gen Physiol. 1955 Mar 20;38(4):549–573. doi: 10.1085/jgp.38.4.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Haid A., Schweyen R. J., Bechmann H., Kaudewitz F., Solioz M., Schatz G. The mitochondrial COB region in yeast codes for apocytochrome b and is mosaic. Eur J Biochem. 1979 Mar;94(2):451–464. doi: 10.1111/j.1432-1033.1979.tb12913.x. [DOI] [PubMed] [Google Scholar]
  11. Jayaraman J., Cotman C., Mahler H. R., Sharp C. W. Biochemical correlates of respiratory deficiency. VII. Glucose repression. Arch Biochem Biophys. 1966 Sep 26;116(1):224–251. doi: 10.1016/0003-9861(66)90029-4. [DOI] [PubMed] [Google Scholar]
  12. Johnston G. C., Pringle J. R., Hartwell L. H. Coordination of growth with cell division in the yeast Saccharomyces cerevisiae. Exp Cell Res. 1977 Mar 1;105(1):79–98. doi: 10.1016/0014-4827(77)90154-9. [DOI] [PubMed] [Google Scholar]
  13. Johnston G. C., Singer R. A., McFarlane S. Growth and cell division during nitrogen starvation of the yeast Saccharomyces cerevisiae. J Bacteriol. 1977 Nov;132(2):723–730. doi: 10.1128/jb.132.2.723-730.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Linnane A. W., Haslam J. M., Lukins H. B., Nagley P. The biogenesis of mitochondria in microorganisms. Annu Rev Microbiol. 1972;26:163–198. doi: 10.1146/annurev.mi.26.100172.001115. [DOI] [PubMed] [Google Scholar]
  16. Mahler H. R., Assimos K., Lin C. C. Derepression in Saccharomyces cerevisiae can be dissociated from cellular proliferation and deoxyribonucleic acid synthesis. J Bacteriol. 1975 Aug;123(2):637–641. doi: 10.1128/jb.123.2.637-641.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mahler H. R., Johnson J. Specific effects of nalidixic acid on mitochondrial gene expression in Saccharomyces cerevisiae. Mol Gen Genet. 1979 Oct 2;176(1):25–31. doi: 10.1007/BF00334291. [DOI] [PubMed] [Google Scholar]
  18. Perlman P. S., Mahler H. R. Derepression of mitochondria and their enzymes in yeast: regulatory aspects. Arch Biochem Biophys. 1974 May;162(1):248–271. doi: 10.1016/0003-9861(74)90125-8. [DOI] [PubMed] [Google Scholar]
  19. Polakis E. S., Bartley W., Meek G. A. Changes in the structure and enzyme activity of Saccharomyces cerevisiae in response to changes in the environment. Biochem J. 1964 Feb;90(2):369–374. doi: 10.1042/bj0900369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Terpstra P., Zanders E., Butow R. A. The association of var1 with the 38 S mitochondrial ribosomal subunit in yeast. J Biol Chem. 1979 Dec 25;254(24):12653–12661. [PubMed] [Google Scholar]
  21. Tzagoloff A. Assembly of the mitochondrial membrane system. IV. Role of mitochondrial and cytoplasmic protein synthesis in the biosynthesis of the rutamycin-sensitive adenosine triphosphatase. J Biol Chem. 1971 May 10;246(9):3050–3056. [PubMed] [Google Scholar]
  22. Tzagoloff A., Macino G., Sebald W. Mitochondrial genes and translation products. Annu Rev Biochem. 1979;48:419–441. doi: 10.1146/annurev.bi.48.070179.002223. [DOI] [PubMed] [Google Scholar]
  23. Warner J. R., Gorenstein C. Yeast has a true stringent response. Nature. 1978 Sep 28;275(5678):338–339. doi: 10.1038/275338a0. [DOI] [PubMed] [Google Scholar]
  24. Woodrow G., Schatz G. The role of oxygen in the biosynthesis of cytochrome c oxidase of yeast mitochondria. J Biol Chem. 1979 Jul 10;254(13):6088–6093. [PubMed] [Google Scholar]

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