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. 1983 Mar;153(3):1562–1566. doi: 10.1128/jb.153.3.1562-1566.1983

Identification of Two Distinct Lactate Dehydrogenases in Rhodospirillum rubrum

Paul R Mueller 1,†, Mary Lynne Perille Collins 1
PMCID: PMC221812  PMID: 6402502

Abstract

The activities of pyridine nucleotide-independent d- and l-lactate dehydrogenases were detected in membranes from Rhodospirillum rubrum grown under aerobic and phototrophic conditions. Crossed immunoelectrophoretic analysis revealed two antigenically distinct enzymes that were further distinguished by specificity for d- and l-stereoisomers of lactate and by the sensitivity of the d-lactate dehydrogenase to inhibition by oxamate and oxalate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnes E. M., Jr, Kaback H. R. Mechanisms of active transport in isolated membrane vesicles. I. The site of energy coupling between D-lactic dehydrogenase and beta-galactoside transport in Escherichia coli membrane vesicles. J Biol Chem. 1971 Sep 10;246(17):5518–5522. [PubMed] [Google Scholar]
  2. Collins M. L., Salton M. R. Solubility characteristics of Micrococcus lysodeikticus membrane components in detergents and chaotropic salts analyzed by immunoelectrophoresis. Biochim Biophys Acta. 1979 May 3;553(1):40–53. doi: 10.1016/0005-2736(79)90029-4. [DOI] [PubMed] [Google Scholar]
  3. Futai M., Kimura H. Inducible membrane-bound L-lactate dehydrogenase from Escherichia coli. Purification and properties. J Biol Chem. 1977 Aug 25;252(16):5820–5827. [PubMed] [Google Scholar]
  4. Futai M. Membrane D-lactate dehydrogenase from Escherichia coli. Purification and properties. Biochemistry. 1973 Jun 19;12(13):2468–2474. doi: 10.1021/bi00737a016. [DOI] [PubMed] [Google Scholar]
  5. GEST H., ORMEROD J. G., ORMEROD K. S. Photometabolism of Rhodospirillum rubrum: light-dependent dissimilation of organic compounds to carbon dioxide and molecular hydrogen by an anaerobic citric acid cycle. Arch Biochem Biophys. 1962 Apr;97:21–33. doi: 10.1016/0003-9861(62)90039-5. [DOI] [PubMed] [Google Scholar]
  6. Garvie E. I. Bacterial lactate dehydrogenases. Microbiol Rev. 1980 Mar;44(1):106–139. doi: 10.1128/mr.44.1.106-139.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Harboe N., Ingild A. Immunization, isolation of immunoglobulins, estimation of antibody titre. Scand J Immunol Suppl. 1973;1:161–164. doi: 10.1111/j.1365-3083.1973.tb03798.x. [DOI] [PubMed] [Google Scholar]
  8. Hillmer P., Gest H. H2 metabolism in the photosynthetic bacterium Rhodopseudomonas capsulata: H2 production by growing cultures. J Bacteriol. 1977 Feb;129(2):724–731. doi: 10.1128/jb.129.2.724-731.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kaback H. R. Molecular biology and energetics of membrane transport. J Cell Physiol. 1976 Dec;89(4):575–593. doi: 10.1002/jcp.1040890414. [DOI] [PubMed] [Google Scholar]
  10. Kohn L. D., Kaback H. R. Mechanisms of active transport in isolated bacterial membrane vesicles. XV. Purification and properties of the membrane-bound D-lactate dehydrogenase from Escherichia coli. J Biol Chem. 1973 Oct 25;248(20):7012–7017. [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Markwell J. P., Lascelles J. Membrane-bound, pyridine nucleotide-independent L-lactate dehydrogenase of Rhodopseudomonas sphaeroides. J Bacteriol. 1978 Feb;133(2):593–600. doi: 10.1128/jb.133.2.593-600.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. ORMEROD J. G., ORMEROD K. S., GEST H. Light-dependent utilization of organic compounds and photoproduction of molecular hydrogen by photosynthetic bacteria; relationships with nitrogen metabolism. Arch Biochem Biophys. 1961 Sep;94:449–463. doi: 10.1016/0003-9861(61)90073-x. [DOI] [PubMed] [Google Scholar]
  14. Owen P., Kaback H. R. Antigenic architecture of membrane vesicles from Escherichia coli. Biochemistry. 1979 Apr 17;18(8):1422–1426. doi: 10.1021/bi00575a005. [DOI] [PubMed] [Google Scholar]
  15. Pratt E. A., Fung L. W., Flowers J. A., Ho C. Membrane-bound D-lactate dehydrogenase from Escherichia coli: purification and properties. Biochemistry. 1979 Jan 23;18(2):312–316. doi: 10.1021/bi00569a013. [DOI] [PubMed] [Google Scholar]
  16. Rudolph F. B., Fromm H. J. Plotting methods for analyzing enzyme rate data. Methods Enzymol. 1979;63:138–159. doi: 10.1016/0076-6879(79)63009-4. [DOI] [PubMed] [Google Scholar]
  17. Weeke B. Crossed immunoelectrophoresis. Scand J Immunol Suppl. 1973;1:47–56. doi: 10.1111/j.1365-3083.1973.tb03778.x. [DOI] [PubMed] [Google Scholar]
  18. Zürrer H., Bachofen R. Hydrogen Production by the Photosynthetic Bacterium Rhodospirillum rubrum. Appl Environ Microbiol. 1979 May;37(5):789–793. doi: 10.1128/aem.37.5.789-793.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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