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. 1977 Oct;132(1):132–138. doi: 10.1128/jb.132.1.132-138.1977

Characterization of a novel thiosulfate-forming enzyme isolated from Desulfovibrio vulgaris.

H L Drake, J M Akagi
PMCID: PMC221836  PMID: 199572

Abstract

An enzyme that formed thiosulfate from bisulfite and trithionate was purified from extracts of Desulfovibrio vulgaris. This enzyme, designated as "thiosulfate-forming" enzyme, required the presence of both bisulfite and trithionate. Various 35S-labeling studies showed that thiosulfate was formed from bisulfite and the inner sulfur atom of trithionate. This involved a nucleophilic attack by the bisulfite ion, resulting in the displacement of the two outer sulfonate groups of trithionate that recycled to participate as free bisulfite in subsequent reactions. This reaction required a reduction, presumably by a concerted mechanism with thiosulfate formation. The natural electron carrier cytochrome c3 participated in this reductive formation of thiosulfate. This reaction was coupled to the bisulfite reductase-catalyzed reaction, which resulted in the reconstruction of a thiosulfate-forming pathway from bisulfite.

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Selected References

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  1. Akagi J. M., Adams V. Isolation of a bisulfite reductase activity from Desulfotomaculum nigrificans and its identification as the carbon monoxide-binding pigment P582. J Bacteriol. 1973 Oct;116(1):392–396. doi: 10.1128/jb.116.1.392-396.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Akagi J. M., Campbell L. L. STUDIES ON THERMOPHILIC SULFATE-REDUCING BACTERIA III. : Adenosine Triphosphate-sulfurylase of Clostridium nigrificans and Desulfovibrio desulfuricans. J Bacteriol. 1962 Dec;84(6):1194–1201. doi: 10.1128/jb.84.6.1194-1201.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Akagi J. M., Chan M., Adams V. Observations on the bisulfite reductase (P582) isolated from Desulfotomaculum nigrificans. J Bacteriol. 1974 Oct;120(1):240–244. doi: 10.1128/jb.120.1.240-244.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Akagi J. M. Electron carries for the phosphoroclastic reaction of Desulfovibrio desulfuricans. J Biol Chem. 1967 May 25;242(10):2478–2483. [PubMed] [Google Scholar]
  5. Asada K., Tamura G., Bandurski R. S. Methyl viologen-linked sulfite reductase from spinach leaves. J Biol Chem. 1969 Sep 25;244(18):4904–4915. [PubMed] [Google Scholar]
  6. Bruschi M., Hatchikian C. E., Golovleva L. A., Gall J. L. Purification and characterization of cytochrome c3, ferredoxin, and rubredoxin isolated from Desulfovibrio desulfuricans Norway. J Bacteriol. 1977 Jan;129(1):30–38. doi: 10.1128/jb.129.1.30-38.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chambers L. A., Trudinger P. A. Are thiosulfate and trithionate intermediates in dissimilatory sulfate reduction? J Bacteriol. 1975 Jul;123(1):36–40. doi: 10.1128/jb.123.1.36-40.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Drake H. L., Akagi J. M. Product analysis of bisulfite reductase activity isolated from Desulfovibrio vulgaris. J Bacteriol. 1976 May;126(2):733–738. doi: 10.1128/jb.126.2.733-738.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Drake H. L., Akagi J. M. Purification of a unique bisulfite-reducing enzyme from Desulfovibrio vulgaris. Biochem Biophys Res Commun. 1976 Aug 23;71(4):1214–1219. doi: 10.1016/0006-291x(76)90783-x. [DOI] [PubMed] [Google Scholar]
  10. Dubourdieu M., Le Gall J. Chemical study of two flavodoxins extracted from sulfate reducing bacteria. Biochem Biophys Res Commun. 1970 Mar 12;38(5):965–972. doi: 10.1016/0006-291x(70)90816-8. [DOI] [PubMed] [Google Scholar]
  11. HORIO T., KAMEN M. D. Preparation and properties of three pure crystalline bacterial haem proteins. Biochim Biophys Acta. 1961 Apr 1;48:266–286. doi: 10.1016/0006-3002(61)90476-0. [DOI] [PubMed] [Google Scholar]
  12. Haschke R. H., Campbell L. L. Thiosulfate reductase of Desulfovibrio vulgaris. J Bacteriol. 1971 May;106(2):603–607. doi: 10.1128/jb.106.2.603-607.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hatchikian E. C. Purification and properties of thiosulfate reductase from Desulfovibrio gigas. Arch Microbiol. 1975 Nov 7;105(3):249–256. doi: 10.1007/BF00447143. [DOI] [PubMed] [Google Scholar]
  14. Jones H. E., Skyring G. W. Reduction of sulphite to sulphide catalysed by desulfoviridin from Desulfovibrio gigas. Aust J Biol Sci. 1974 Feb;27(1):7–14. [PubMed] [Google Scholar]
  15. Knight E., Jr, Hardy R. W. Isolation and characteristics of flavodoxin from nitrogen-fixing Clostridium pasteurianum. J Biol Chem. 1966 Jun 25;241(12):2752–2756. [PubMed] [Google Scholar]
  16. Kobayashi K., Seki Y., Ishimoto M. Biochemical studies on sulfate-ruducing bacteria. 8. Sulfite reductase from Desulfovibrio vulgaris--mechanism of trithionate, thiosulfate, and sulfide formation and enzymatic properties. J Biochem. 1974 Mar;75(3):519–529. doi: 10.1093/oxfordjournals.jbchem.a130420. [DOI] [PubMed] [Google Scholar]
  17. Kobayashi K., Tachibana S., Ishimoto M. Intermediary formation of trithionate in sulfite reduction by a sulfate-reducing bacterium. J Biochem. 1969 Jan;65(1):155–157. [PubMed] [Google Scholar]
  18. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  19. Lee J. P., LeGall J., Peck H. D., Jr Isolation of assimilatroy- and dissimilatory-type sulfite reductases from Desulfovibrio vulgaris. J Bacteriol. 1973 Aug;115(2):529–542. doi: 10.1128/jb.115.2.529-542.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Lee J. P., Peck H. D., Jr Purification of the enzyme reducing bisulfite to trithionate from Desulfovibrio gigas and its identification as desulfoviridin. Biochem Biophys Res Commun. 1971 Nov 5;45(3):583–589. doi: 10.1016/0006-291x(71)90457-8. [DOI] [PubMed] [Google Scholar]
  21. Nakatsukasa W., Akagi J. M. Thiosulfate reductase isolated from Desulfotomaculum nigrificans. J Bacteriol. 1969 May;98(2):429–433. doi: 10.1128/jb.98.2.429-433.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schmidt A., Abrams W. R., Schiff J. A. Reduction of adenosine 5'-phosphosulfate to cysteine in extracts from Chlorella and mutants blocked for sulfate reduction. Eur J Biochem. 1974 Sep 16;47(3):423–434. doi: 10.1111/j.1432-1033.1974.tb03709.x. [DOI] [PubMed] [Google Scholar]
  23. Schmidt A. Sulfate reduction in a cell-free system of Chlorella. The ferredoxin dependent reduction of a protein-bound intermediate by a thiosulfonate reductase. Arch Mikrobiol. 1973 Oct 4;93(1):29–52. [PubMed] [Google Scholar]
  24. Suh B., Akagi J. M. Formation of thiosulfate from sulfite by Desulfovibrio vulgaris. J Bacteriol. 1969 Jul;99(1):210–215. doi: 10.1128/jb.99.1.210-215.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tsang M. L., Schiff J. A. Sulfate-reducing pathway in Escherichia coli involving bound intermediates. J Bacteriol. 1976 Mar;125(3):923–933. doi: 10.1128/jb.125.3.923-933.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Yoshimoto A., Sato R. Studies on yeast sulfite reductase. 3. Further characterization. Biochim Biophys Acta. 1970 Nov 11;220(2):190–205. doi: 10.1016/0005-2744(70)90005-7. [DOI] [PubMed] [Google Scholar]

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