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. 1977 Nov;132(2):549–554. doi: 10.1128/jb.132.2.549-554.1977

Further mapping of several membrane lipid biosynthetic genes (fabC, fabB, gpsA, plsB) of Escherichia coli.

D Clark, J E Cronan Jr
PMCID: PMC221895  PMID: 334747

Abstract

New genetic mapping data on several loci involved in membrane lipid synthesis are reported. We demonstrated that the lesion designated fabC by Broekman and Hoeckstra (Mol. Gen. Genet. 124:65-67, 1975) is a fabB mutation. In the course of this work, the orientation of the pdxB and fabB loci was determined. The order of the loci is fabB pdxB purF. We also report cotransduction between the gpsA and cysE loci and show that the order of these markers is mtl cysE gpsA. Cotransduction between the plsB and kdgK loci was also sought. Despite extensive experiments, we were unable to detect cotransduction between these loci. In addition, we were unable to detect cotransduction among several markers in region 46 to 48 min of the map.

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Selected References

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  1. Akamatsu Y. Osmotic stabilization of unsaturated fatty acid auxotrophs of Escherichia coli. J Biochem. 1974 Sep;76(3):553–561. doi: 10.1093/oxfordjournals.jbchem.a130599. [DOI] [PubMed] [Google Scholar]
  2. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bell R. M. Mutants of Escherichia coli defective in membrane phospholipid synthesis: macromolecular synthesis in an sn-glycerol 3-phosphate acyltransferase Km mutant. J Bacteriol. 1974 Mar;117(3):1065–1076. doi: 10.1128/jb.117.3.1065-1076.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Blumenthal T. P1 transduction: formation of heterogenotes upon cotransduction of bacterial genes with a P2 prophage. Virology. 1972 Jan;47(1):76–93. doi: 10.1016/0042-6822(72)90241-3. [DOI] [PubMed] [Google Scholar]
  5. Broekman J. H., Hoekstra W. P. Mapping of fabC, a locus for the biosynthesis of unsaturated fatty acids in Escherichia coli K12. Mol Gen Genet. 1973 Jul 31;124(1):65–67. doi: 10.1007/BF00267165. [DOI] [PubMed] [Google Scholar]
  6. Broekman J. H., Steenbakkers J. F. Effect of the osmotic pressure of the growth medium on fabB mutants of Escherichia coli. J Bacteriol. 1974 Mar;117(3):971–977. doi: 10.1128/jb.117.3.971-977.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cozzarelli N. R., Freedberg W. B., Lin E. C. Genetic control of L-alpha-glycerophosphate system in Escherichia coli. J Mol Biol. 1968 Feb 14;31(3):371–387. doi: 10.1016/0022-2836(68)90415-4. [DOI] [PubMed] [Google Scholar]
  8. Cozzarelli N. R., Koch J. P., Hayashi S., Lin E. C. Growth stasis by accumulated L-alpha-glycerophosphate in Escherichia coli. J Bacteriol. 1965 Nov;90(5):1325–1329. doi: 10.1128/jb.90.5.1325-1329.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cronan J. E., Jr, Bell R. M. Mutants of Escherichia coli defective in membrane phospholipid synthesis: mapping of sn-glycerol 3-phosphate acyltransferase Km mutants. J Bacteriol. 1974 Oct;120(1):227–233. doi: 10.1128/jb.120.1.227-233.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Cronan J. E., Jr, Bell R. M. Mutants of Escherichia coli defective in membrane phospholipid synthesis: mapping of the structural gene for L-glycerol 3-phosphate dehydrogenase. J Bacteriol. 1974 May;118(2):598–605. doi: 10.1128/jb.118.2.598-605.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Cronan J. E., Jr, Birge C. H., Vagelos P. R. Evidence for two genes specifically involved in unsaturated fatty acid biosynthesis in Escherichia coli. J Bacteriol. 1969 Nov;100(2):601–604. doi: 10.1128/jb.100.2.601-604.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Cronan J. E., Jr, Gelmann E. P. An estimate of the minimum amount of unsaturated fatty acid required for growth of Escherichia coli. J Biol Chem. 1973 Feb 25;248(4):1188–1195. [PubMed] [Google Scholar]
  13. Cronan J. E., Jr, Gelmann E. P. Physical properties of membrane lipids: biological relevance and regulation. Bacteriol Rev. 1975 Sep;39(3):232–256. doi: 10.1128/br.39.3.232-256.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Cronan J. E., Jr, Silbert D. F., Wulff D. L. Mapping of the fabA locus for unsaturated fatty acid biosynthesis in Escherichia coli. J Bacteriol. 1972 Oct;112(1):206–211. doi: 10.1128/jb.112.1.206-211.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Dempsey W. B. Characterization of pyridoxine auxotrophs of Escherichia coli: chromosomal position of linkage group I. J Bacteriol. 1969 Oct;100(1):295–300. doi: 10.1128/jb.100.1.295-300.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Epstein W., Fox C. F. Mapping of a locus for unsaturated fatty acid biosynthesis in Escherichia coli. J Bacteriol. 1970 Jul;103(1):273–274. doi: 10.1128/jb.103.1.273-274.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Essenberg R. C., Kornberg H. L. Location of the gene specifying hexose phosphate transport (uhp) on the chromosome of Escherichia coli. J Gen Microbiol. 1977 Mar;99(1):157–169. doi: 10.1099/00221287-99-1-157. [DOI] [PubMed] [Google Scholar]
  18. Ferenci T., Kornberg H. L. The utilization of fructose by Escherichia coli. Properties of a mutant defective in fructose 1-phosphate kinase activity. Biochem J. 1973 Feb;132(2):341–347. doi: 10.1042/bj1320341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Hane M. W., Wood T. H. Escherichia coli K-12 mutants resistant to nalidixic acid: genetic mapping and dominance studies. J Bacteriol. 1969 Jul;99(1):238–241. doi: 10.1128/jb.99.1.238-241.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Jones-Mortimer M. C. Positive control of sulphate reduction in Escherichia coli. Isolation, characterization and mapping oc cysteineless mutants of E. coli K12. Biochem J. 1968 Dec;110(3):589–595. doi: 10.1042/bj1100589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Konrad E. B., Lehman I. R. Novel mutants of Escherichia coli that accumulate very small DNA replicative intermediates. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2150–2154. doi: 10.1073/pnas.72.6.2150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. McFall E. Mapping of the d-serine deaminase region in Escherichia coli K-12. Genetics. 1967 Jan;55(1):91–99. doi: 10.1093/genetics/55.1.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Oeschger M. P., Berlyn M. K. A simple procedure for localized mutagenesis using nitrosoguanidine. Mol Gen Genet. 1974;134(1):77–83. doi: 10.1007/BF00332814. [DOI] [PubMed] [Google Scholar]
  24. Overath P., Hill F. F., Lamnek-Hirsch I. Biogenesis of E. coli membrane: evidence for randomization of lipid phase. Nat New Biol. 1971 Dec 29;234(52):264–267. doi: 10.1038/newbio234264a0. [DOI] [PubMed] [Google Scholar]
  25. Pouyssegur J., Stoeber F. Genetic control of the 2-keto-3-deoxy-d-gluconate metabolism in Escherichia coli K-12: kdg regulon. J Bacteriol. 1974 Feb;117(2):641–651. doi: 10.1128/jb.117.2.641-651.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Rodolakis A., Casse F., Starka J. Morphological mutants of Escherichia coli K12. Mapping of the env C mutation. Mol Gen Genet. 1974 May 21;130(2):177–181. doi: 10.1007/BF00269088. [DOI] [PubMed] [Google Scholar]
  27. Solomon E., Lin E. C. Mutations affecting the dissimilation of mannitol by Escherichia coli K-12. J Bacteriol. 1972 Aug;111(2):566–574. doi: 10.1128/jb.111.2.566-574.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

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