Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1977 Nov;132(2):576–583. doi: 10.1128/jb.132.2.576-583.1977

Transformation in pneumococcus: existence and properties of a complex involving donor deoxyribonucleate single strands in eclipse.

D A Morrison
PMCID: PMC221898  PMID: 21166

Abstract

Donor deoxyribonucleic acid (DNA) single strands exist in a complex during the eclipse phase in pneumococcal transformation. This eclipse complex exhibited specific physical properties distinct from those of both pure DNA single strands and native DNA. These included a lower affinity for diethylaminoethyl-cellulose and hydroxylapatite than that of single-strand DNA, faster sedimentation than the DNA chains that it contains, and a buoyant density in Cs2SO4 lower than that of native DNA. The complex was dissociated by treatments with sodium dodecyl sulfate, NaOH, guanidine-hydrochloride, chloroform, and proteinase K but was insensitive to ribonuclease.

Full text

PDF
576

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cato A., Jr, Guild W. R. Transformation and DNA size. I. Activity of fragments of defined size and a fit to a random double cross-over model. J Mol Biol. 1968 Oct 14;37(1):157–178. doi: 10.1016/0022-2836(68)90080-6. [DOI] [PubMed] [Google Scholar]
  2. Collins C. J., Guild W. R. Events occurring near the time of synapsis during transformation in Diplococcus pneumoniae. J Bacteriol. 1972 Jan;109(1):266–275. doi: 10.1128/jb.109.1.266-275.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Davidoff-Abelson R., Dubnau D. Conditions affecting the isolation from transformed cells of Bacillus subtilis of high-molecular-weight single-stranded deoxyribonucleic acid of donor origin. J Bacteriol. 1973 Oct;116(1):146–153. doi: 10.1128/jb.116.1.146-153.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Davidoff-Abelson R., Dubnau D. Kinetic analysis of the products of donor deoxyribonucleate in transformed cells of Bacillus subtilis. J Bacteriol. 1973 Oct;116(1):154–162. doi: 10.1128/jb.116.1.154-162.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dubnau D., Cirigliano C. Fate of transforming DNA following uptake by competent Bacillus subtilis. Formation and properties of products isolated from transformed cells which are derived entirely from donor DNA. J Mol Biol. 1972 Feb 28;64(1):9–29. doi: 10.1016/0022-2836(72)90318-x. [DOI] [PubMed] [Google Scholar]
  6. EPHRUSSI-TAYLOR H. [The status of the transforming DNA during the 1st phases of bacterial transformation]. C R Seances Soc Biol Fil. 1960;154:1951–1955. [PubMed] [Google Scholar]
  7. Eisenstadt E., Lange R., Willecke K. Competent Bacillus subtilis cultures synthesize a denatured DNA binding activity. Proc Natl Acad Sci U S A. 1975 Jan;72(1):323–327. doi: 10.1073/pnas.72.1.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FOX M. S., ALLEN M. K. ON THE MECHANISM OF DEOXYRIBONUCLEATE INTEGRATION IN PNEUMOCOCCAL TRANSFORMATION. Proc Natl Acad Sci U S A. 1964 Aug;52:412–419. doi: 10.1073/pnas.52.2.412. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. FOX M. S., HOTCHKISS R. D. Fate of transforming deoxyribonucleate following fixation by transformable bacteria. Nature. 1960 Sep 17;187:1002–1006. doi: 10.1038/1871002a0. [DOI] [PubMed] [Google Scholar]
  10. GUILD W. R., ROBINSON M. Evidence for message reading from a unique strand of pneumococcal DNA. Proc Natl Acad Sci U S A. 1963 Jul;50:106–112. doi: 10.1073/pnas.50.1.106. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. GUILD W. R. Transformation by denatured deoxyribonucleic acid. Proc Natl Acad Sci U S A. 1961 Oct 15;47:1560–1564. doi: 10.1073/pnas.47.10.1560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ghei O. K., Lacks S. A. Recovery of donor deoxyribonucleic acid marker activity from eclipse in pneumococcal transformation. J Bacteriol. 1967 Mar;93(3):816–829. doi: 10.1128/jb.93.3.816-829.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gurney T., Jr, Fox M. S. Physical and genetic hybrids formed in bacterial transformation. J Mol Biol. 1968 Feb 28;32(1):83–100. doi: 10.1016/0022-2836(68)90147-2. [DOI] [PubMed] [Google Scholar]
  14. HJERTEN S., LEVIN O., TISELIUS A. Protein chromatography on calcium phosphate columns. Arch Biochem Biophys. 1956 Nov;65(1):132–155. doi: 10.1016/0003-9861(56)90183-7. [DOI] [PubMed] [Google Scholar]
  15. LACKS S. Molecular fate of DNA in genetic transformation of Pneumococcus. J Mol Biol. 1962 Jul;5:119–131. doi: 10.1016/s0022-2836(62)80067-9. [DOI] [PubMed] [Google Scholar]
  16. LERMAN L. S., TOLMACH L. J. Genetic transformation. I. Cellular incorporation of DNA accompanying transformation in Pneumococcus. Biochim Biophys Acta. 1957 Oct;26(1):68–82. doi: 10.1016/0006-3002(57)90055-0. [DOI] [PubMed] [Google Scholar]
  17. Lacks S., Greenberg B. Competence for deoxyribonucleic acid uptake and deoxyribonuclease action external to cells in the genetic transformation of Diplococcus pneumoniae. J Bacteriol. 1973 Apr;114(1):152–163. doi: 10.1128/jb.114.1.152-163.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lacks S., Greenberg B., Neuberger M. Role of a deoxyribonuclease in the genetic transformation of Diplococcus pneumoniae. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2305–2309. doi: 10.1073/pnas.71.6.2305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Miao R., Guild W. R. Competent Diplococcus pneumoniae accept both single- and double-stranded deoxyribonucleic acid. J Bacteriol. 1970 Feb;101(2):361–364. doi: 10.1128/jb.101.2.361-364.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Morrison D. A., Guild W. R. Breakage prior to entry of donor DNA in Pneumococcus transformation. Biochim Biophys Acta. 1973 Apr 11;299(4):545–556. doi: 10.1016/0005-2787(73)90226-8. [DOI] [PubMed] [Google Scholar]
  21. Morrison D. A., Guild W. R. Transformation and deoxyribonucleic acid size: extent of degradation on entry varies with size of donor. J Bacteriol. 1972 Dec;112(3):1157–1168. doi: 10.1128/jb.112.3.1157-1168.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Piechowska M., Fox M. S. Fate of transforming deoxyribonucleate in Bacillus subtilis. J Bacteriol. 1971 Nov;108(2):680–689. doi: 10.1128/jb.108.2.680-689.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Porter R. D., Guild W. R. Number of transformable units per cell in Diplococcus pneumoniae. J Bacteriol. 1969 Mar;97(3):1033–1035. doi: 10.1128/jb.97.3.1033-1035.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. ROWND R., LANYI J., DOTY P. The biological and physical heterogeneity of thermally denatured and renatured deoxyribonucleic acid. Biochim Biophys Acta. 1961 Oct 14;53:225–227. doi: 10.1016/0006-3002(61)90815-0. [DOI] [PubMed] [Google Scholar]
  25. Shoemaker N. B., Guild W. R. Destruction of low efficiency markers is a slow process occurring at a heteroduplex stage of transformation. Mol Gen Genet. 1974;128(4):283–290. doi: 10.1007/BF00268516. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES