Abstract
Cells of Arthrobacter atrocyaneus and A. crystallopoietes, harvested during their exponential phase, were starved in 0.03 M phosphate buffer (pH 7.0) for 28 days. During this time, the cells maintained 90 to 100% viability. Experimental results were similar for both organisms. Total cellular deoxyribonucleic acid was maintained. Measurable degradation rates for deoxyribonucleic acid as determined by radioisotope techniques were not observed, and only during the initial hours of starvation could a synthetic rate be determined. Total ribonucleic acid levels remained stable for the first 24 h of starvation, after which slow, continuous loss of orcinol-reactive material occurred. Synthetic and degradative rates of ribonucleic acid, as determined by radioisotope techniques, dropped quickly at the onset of starvation. Constant basal rates were attained after 24 h. In A. atrocyaneus, total cell protein was degraded continuously from the onset of starvation. In A. crystallopoietes, total cell protein remained stable for the first 24 h, after which slow continuous loss occurred. After 28 days, the total protein per cell was similar for both organisms. In the first week, amino acid pools stabilized at about 50% of the values characteristic of growth. Rates of degradation of protein decreased rapidly for the first 24 h for both organisms, but leveled to a constant basal rate thereafter. Rates of new protein synthesis dropped during the first 24 h and by 48 h achieved a constant basal rate.
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Selected References
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- BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ben-Hamida F., Schlessinger D. Synthesis and breakdown of ribonucleic acid in Escherichia coli starving for nitrogen. Biochim Biophys Acta. 1966 Apr 18;119(1):183–191. doi: 10.1016/0005-2787(66)90049-9. [DOI] [PubMed] [Google Scholar]
- Boylen C. W., Ensign J. C. Intracellular substrates for endogenous metabolism during long-term starvation of rod and spherical cells of Arthrobacter crystallopoietes. J Bacteriol. 1970 Sep;103(3):578–587. doi: 10.1128/jb.103.3.578-587.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Boylen C. W., Pate J. L. Fine structure of Arthrobacter crystallopoietes during long-term starvation of rod and spherical stage cells. Can J Microbiol. 1973 Jan;19(1):1–5. doi: 10.1139/m73-001. [DOI] [PubMed] [Google Scholar]
- Burleigh I. G., Dawes E. A. Studies on the endogenous metabolism and senescence of starved Sarcina lutea. Biochem J. 1967 Jan;102(1):236–250. doi: 10.1042/bj1020236. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CERIOTTI G. Determination of nucleic acids in animal tissues. J Biol Chem. 1955 May;214(1):59–70. [PubMed] [Google Scholar]
- Ensign J. C. Long-term starvation survival of rod and spherical cells of Arthrobacter crystallopoietes. J Bacteriol. 1970 Sep;103(3):569–577. doi: 10.1128/jb.103.3.569-577.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evans I. M., Eberle H. Accumulation of the capacity of initiation of deoxyribonucleic acid replication in Escherichia coli. J Bacteriol. 1975 Mar;121(3):883–891. doi: 10.1128/jb.121.3.883-891.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hanna M. H., Carl P. L. Reinitiation of deoxyribonucleic acid synthesis by deoxyribonucleic acid initiation mutants of Escherichia coli: role of ribonucleic acid synthesis, protein synthesis, and cell division. J Bacteriol. 1975 Jan;121(1):219–226. doi: 10.1128/jb.121.1.219-226.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hempfling W. P., Mainzer S. E. Effects of varying the carbon source limiting growth on yield and maintenance characteristics of Escherichia coli in continuous culture. J Bacteriol. 1975 Sep;123(3):1076–1087. doi: 10.1128/jb.123.3.1076-1087.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROBERTS N. R., LEINER K. Y., WU M. L., FARR A. L. The quantitative histochemistry of brain. I. Chemical methods. J Biol Chem. 1954 Mar;207(1):1–17. [PubMed] [Google Scholar]
- MANDELSTAM J. The intracellular turnover of protein and nucleic acids and its role in biochemical differentiation. Bacteriol Rev. 1960 Sep;24(3):289–308. doi: 10.1128/br.24.3.289-308.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MacKelvie R. M., Campbell J. J., Gronlund A. F. Survival and intracellular changes of Pseudomonas aeruginosa during prolonged starvation. Can J Microbiol. 1968 Jun;14(6):639–645. doi: 10.1139/m68-107. [DOI] [PubMed] [Google Scholar]
- STRANGE R. E., WADE H. E., NESS A. G. The catabolism of proteins and nucleic acids in starved Aerobacter aerogenes. Biochem J. 1963 Feb;86:197–203. doi: 10.1042/bj0860197. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thomas T. D., Batt R. D. Degradation of cell constituents by starved Streptococcus lactis in relation to survival. J Gen Microbiol. 1969 Nov;58(3):347–362. doi: 10.1099/00221287-58-3-347. [DOI] [PubMed] [Google Scholar]
- Thomas T. D., Batt R. D. Synthesis of protein and ribonucleic acid by starved Streptococcus lactis in relation to survival. J Gen Microbiol. 1969 Nov;58(3):363–369. doi: 10.1099/00221287-58-3-363. [DOI] [PubMed] [Google Scholar]