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. 1977 Nov;132(2):590–595. doi: 10.1128/jb.132.2.590-595.1977

Cleavage of adenosine 5'-monophosphate during uptake by Streptomyces griseus.

J F Martin, A L Demain
PMCID: PMC221900  PMID: 410793

Abstract

Unlabeled adenine brought about a (delayed) decrease in radioactivity that had been taken up by phosphate-limited resting cells of Streptomyces griseus from [14C]adenine-labeled adenosine 5'-monophosphate (AMP). Inorganic phosphate, on the other hand, stimulated adenine uptake from AMP, presumably by activating an energy-dependent active transport mechanism. Unlabeled phosphate rapidly diluted the uptake of radioactivity from [32P]AMP. Adenine inhibited uptake of [32P]AMP but not that of [32P]orthophosphate; adenine is thought to act by inhibiting the cleavage of AMP. The uptake of 32P and 14C from double-labeled AMP showed marked differences; 32P was taken up much faster into both cells and nucleic acids. These data indicate that uptake of AMP components takes place after extracellular dephosphorylation of the nucleotide.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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