Abstract
When Bacillus megaterium spores germinate in the absence of an exogenous carbon source, the first minutes of germination are accompanied by production of large amounts (approximately 70 nmol/mg of dry spores) of acetate and much smaller amounts of pyruvate and lactate. The majority of these compounds are excreted into the medium. Exogenous pyruvate and alanine are also converted to CO2 and acetate by germinating spores, presumably by using the pyruvate dehydrogenase that is present in dormant spores. These data suggest that the 3-phosphoglyceric acid stores in the dormant spore and alanine generated by proteolysis early in germination can be catabolized to acetate during germination with production of large amounts of reduced nicotinamide adenine dinucleotide, acetyl coenzyme A, and adenosine 5'-triphosphate.
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- CHURCH B. D., HALVORSON H. Intermediate metabolism of aerobic spores. I. Activation of glucose oxidation in spores of Bacillus cereus var terminalis. J Bacteriol. 1957 Apr;73(4):470–476. doi: 10.1128/jb.73.4.470-476.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Freese E., Fortnagel U. Growth and sporulation of Bacillus subtilis mutants blocked in the pyruvate dehydrogenase complex. J Bacteriol. 1969 Sep;99(3):745–756. doi: 10.1128/jb.99.3.745-756.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kornberg A., Spudich J. A., Nelson D. L., Deutscher M. P. Origin of proteins in sporulation. Annu Rev Biochem. 1968;37:51–78. doi: 10.1146/annurev.bi.37.070168.000411. [DOI] [PubMed] [Google Scholar]
- Nickerson K. W., Bulla L. A., Jr, Mounts T. L. Lipid metabolism during bacterial growth, sporulation, and germination: differential synthesis of individual branched- and normal-chain fatty acids during spore germination and outgrowth of Bacillus thuringiensis. J Bacteriol. 1975 Dec;124(3):1256–1262. doi: 10.1128/jb.124.3.1256-1262.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Setlow B., Setlow P. Levels of acetyl coenzyme A, reduced and oxidized coenzyme A, and coenzyme A in disulfide linkage to protein in dormant and germinated spores and growing and sporulating cells of Bacillus megaterium. J Bacteriol. 1977 Nov;132(2):444–452. doi: 10.1128/jb.132.2.444-452.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Setlow B., Setlow P. Levels of oxidized and reduced pyridine nucleotides in dormant spores and during growth, sporulation, and spore germination of Bacillus megaterium. J Bacteriol. 1977 Feb;129(2):857–865. doi: 10.1128/jb.129.2.857-865.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Setlow P., Kornberg A. Biochemical studies of bacterial sporulation and germination. 23. Nucleotide metabolism during spore germination. J Biol Chem. 1970 Jul 25;245(14):3645–3652. [PubMed] [Google Scholar]
- Setlow P., Kornberg A. Biochemical studies of bacterial sporulation and germination. XVII. Sulfhydryl and disulfide levels in dormancy and germination. J Bacteriol. 1969 Dec;100(3):1155–1160. doi: 10.1128/jb.100.3.1155-1160.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Setlow P., Primus G. Protein metabolism during germination of Bacillus megaterium spores. I. Protein synthesis and amino acid metabolism. J Biol Chem. 1975 Jan 25;250(2):623–630. [PubMed] [Google Scholar]
- Setlow P. Spermidine biosynthesis during germination and subsequent vegetative growth of Bacillus megaterium spores. J Bacteriol. 1974 Oct;120(1):311–315. doi: 10.1128/jb.120.1.311-315.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]